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The map label for this gene is folA [H]

Identifier: 28199247

GI number: 28199247

Start: 1586106

End: 1586606

Strand: Reverse

Name: folA [H]

Synonym: PD1363

Alternate gene names: 28199247

Gene position: 1586606-1586106 (Counterclockwise)

Preceding gene: 28199248

Following gene: 28199246

Centisome position: 62.97

GC content: 54.29

Gene sequence:

>501_bases
ATGATTGTTTCCTTAATTGCGGCGTTGGATCGTTACCGCGCCATTGGTCGTGATAATGGGTTGCCGTGGCACTTGCCGGA
CGATCTGAAGCGTTTCAAAGCATTGACTTTAGGTCGACCGGTGCTGATGGGACGCAAGACTGCGCATTCCCTTGGACGTC
CCTTACCAGGGAGAACCAATCTGGTATTGACGCGTTCTGGACACGTGCCATTTTCTGAAATGCGGGCGGTGGATTCGCTT
GATGCAGCTATCGTCCAGGCGGCTGCGGAGGGTGCCGACACGTTAAGTGTGATTGGTGGAGGTGAACTGTATGCACTGAC
TTTGCCGCACGCCAATGCGATGTACTTAACTCACGTGGATGTGGCTGTTGCGGGCGCTGACACGTATTTTCCTATGTTCG
ACGCCGTCGATTGGGAGATCAAGAACCGTGAACCCCATCAGGGCGATGGGCGTCATGCCTACCCGTTCACCTTCATAGAC
TATTGCCGCCGATTGCGTTAG

Upstream 100 bases:

>100_bases
ATCAGTTGGCGACTCAGACCAGTCGGCGTAATGAGTGGAGACGCATCTCAAGAAAGCCAGAGAAAATAATGAATATCTAC
AACGTGTTGTACGTAGTGCC

Downstream 100 bases:

>100_bases
TGCATTCCCACGATCTTGTGTTTCTGCAACGCATGCAGTGAGCAGTGACGGGATGCGCGGTGGCTATTGTTCATCAACAG
TATTCAATGAAGTGCGATTG

Product: dihydrofolate reductase type III

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGRTNLVLTRSGHVPFSEMRAVDSL
DAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDWEIKNREPHQGDGRHAYPFTFID
YCRRLR

Sequences:

>Translated_166_residues
MIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGRTNLVLTRSGHVPFSEMRAVDSL
DAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDWEIKNREPHQGDGRHAYPFTFID
YCRRLR
>Mature_166_residues
MIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGRTNLVLTRSGHVPFSEMRAVDSL
DAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDWEIKNREPHQGDGRHAYPFTFID
YCRRLR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=165, Percent_Identity=44.8484848484849, Blast_Score=130, Evalue=5e-32,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18338; Mature: 18338

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGRTN
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHCCCCCCCCC
LVLTRSGHVPFSEMRAVDSLDAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVD
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEEEEE
VAVAGADTYFPMFDAVDWEIKNREPHQGDGRHAYPFTFIDYCRRLR
EEEECCCCCCCCCCCCCEEECCCCCCCCCCCEECCHHHHHHHHHCC
>Mature Secondary Structure
MIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGRTN
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHCCCCCCCCC
LVLTRSGHVPFSEMRAVDSLDAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVD
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEEEEE
VAVAGADTYFPMFDAVDWEIKNREPHQGDGRHAYPFTFIDYCRRLR
EEEECCCCCCCCCCCCCEEECCCCCCCCCCCEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]