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The map label for this gene is yibK [H]

Identifier: 28198735

GI number: 28198735

Start: 1029227

End: 1029688

Strand: Reverse

Name: yibK [H]

Synonym: PD0830

Alternate gene names: 28198735

Gene position: 1029688-1029227 (Counterclockwise)

Preceding gene: 28198742

Following gene: 28198734

Centisome position: 40.86

GC content: 54.98

Gene sequence:

>462_bases
ATGCCTCTCTACGATGTGCTGCTGTACCAGCCCGAAATACCCCCCAACACCGGCAATGTCATCCGCCTGTGTGCCAATAC
TGGCGCGCGGCTACATTTGATCGAACCACTCGGTTTTGATCTGGATGACCGGCAACTCAAGCGTGCTGGATTGGACTATC
ACGAATACGCCCGGATGCAGGTACATCCCAACCTGAGCAGTGCACTGGCTGCAATCGCACCACGACGCCTATTTGCGCTC
AGTACACGTGGCCAAGTGCGCTATGACACCCCGACTTTCCGCACGGGAGATGCTTTTATTTTCGGTCCGGAAACCCGCGG
TTTGCCAGACACTGTGTTGCATACCATCCCGGAGTCTCAACGCCTGTATTTACCGATGCAACCACAAAGCCGCAGCTTAA
ACCTCTCCAATGCCGTCGCGGTGATGGTATTTGAGGCATGGCGGCAACAGGGTTTTCAATGA

Upstream 100 bases:

>100_bases
GAGCGTCACCATCATTGATGCGTTACTCTGCTCATTAAGATGCGCCGAACAAATTAAACAGTGGCTGCCTCGACGCTGAC
GCTACACTGATCTCATGTCC

Downstream 100 bases:

>100_bases
CAATGACAATGACAATGACAATGACAATGACAATATTGTGCTGATCTTGCTCATGAGCAACACTACGTTAAGCCAGTGAA
GCTAAGAGCGTGCTTTTATT

Product: tRNA/rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQVHPNLSSALAAIAPRRLFAL
STRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ

Sequences:

>Translated_153_residues
MPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQVHPNLSSALAAIAPRRLFAL
STRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ
>Mature_152_residues
PLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQVHPNLSSALAAIAPRRLFALS
TRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=150, Percent_Identity=55.3333333333333, Blast_Score=188, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17321; Mature: 17190

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00104 EPSP_SYNTHASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQ
CCCCEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHEEEEE
VHPNLSSALAAIAPRRLFALSTRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQ
ECCCHHHHHHHHCCHHEEEECCCCEEEECCCCEECCCEEEECCCCCCCCHHHHHHCCCCC
RLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQ
CCCEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHEEEEE
VHPNLSSALAAIAPRRLFALSTRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQ
ECCCHHHHHHHHCCHHEEEECCCCEEEECCCCEECCCEEEECCCCCCCCHHHHHHCCCCC
RLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA