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The map label for this gene is kpsF [H]

Identifier: 28198550

GI number: 28198550

Start: 789309

End: 790310

Strand: Reverse

Name: kpsF [H]

Synonym: PD0642

Alternate gene names: 28198550

Gene position: 790310-789309 (Counterclockwise)

Preceding gene: 28198554

Following gene: 28198549

Centisome position: 31.36

GC content: 55.59

Gene sequence:

>1002_bases
ATGGCTGTCTCATTACTCCAGCACAATCACCTCAGCGATACAGCGCTGATCGCAAGCGCAAGAAGGGTGATCGAGATTGA
GCGTGAGGCGCTGACATTACTCAACGAACGGATAGGCGCACCATTTGTGGCAGCCTGTCGGCTGATTCTGAACTCGCACG
GCCGCGTGATCAGCACTGGCATGGGCAAATCCGGACACATTGCACGCAAGATCGCTGCCACCTTGGCTTCAACGGGTACA
CCCGCATTTTTCGTACATCCCGGCGAAGCTGGGCATGGCGACCTTGGAATGATCACGGACAGCGATGTGATCCTGGCGTT
GTCCTACTCAGGTGAATCAGACGAAGTACGGATGCTGCTTCCAGTCCTCAAGCGTCAAGGCAATCCAATCATTGCCATGA
CCGGCCGCCCCCAATCAACACTGGCACAGGCTGCCGATGTGCATCTGGATGTCAGTGTCACCACCGAAGCCTGCCCACTG
GATCTAGCGCCAACCTCCAGCACAACCACTTCGCTAGTAATTGGCGACGCACTCGCAGTCGCACTATTGGATGCACGCGG
CTTTACTGCGGAAGATTTTGCCCGCTCACACCCCGCTGGCCACCTAGGTCGACGCCTACTACTGCACATTACGGACGTGA
TGCACAGTGGTGATGACCTGCCAGCAGTACATGAGGAAGCCACACTGAGCGAGGCATTACTCGAGATGACTCGCAAACGT
CTGGGCATGACTGCAATCGTTGACAACGAGGGCCGACTCCAGGGAGTGTTCACTGACGGCGACTTGCGCCGTGCTCTGGA
CAGCAACATTGATGTAAGAAACGCGCGCATCAACGAAGTCATGACCCGCCATCCCAAAACAATCAATGCCGACCAACTCG
CGGCCGAAGCTGCACGATTGATGGAAGCCAATAAAATCAATGGTTTGATTGTGGTCGATCCGCAACAGCGTGCGGTAGGC
GCCCTGAACATTCACGACCTGCTGCACGCCAAAATTGTTTAA

Upstream 100 bases:

>100_bases
ACCAGACTCAATAAGTTTACGAATAGTTTCAGCGTCCAATAGACACGTCTCTAAATCTAATAAAATGGAGGTTCATTTTA
CTCCTGTACTGAACACATGT

Downstream 100 bases:

>100_bases
TCCTCTGAAAAATACCAATGCCTTATTCCCCATTAAATACCCTGCCTGCCGATGTCATTCAACGTGCAAAATGCATCCGC
TTAGCATGCTTTGACGTGGA

Product: polysialic acid capsule expression protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAVSLLQHNHLSDTALIASARRVIEIEREALTLLNERIGAPFVAACRLILNSHGRVISTGMGKSGHIARKIAATLASTGT
PAFFVHPGEAGHGDLGMITDSDVILALSYSGESDEVRMLLPVLKRQGNPIIAMTGRPQSTLAQAADVHLDVSVTTEACPL
DLAPTSSTTTSLVIGDALAVALLDARGFTAEDFARSHPAGHLGRRLLLHITDVMHSGDDLPAVHEEATLSEALLEMTRKR
LGMTAIVDNEGRLQGVFTDGDLRRALDSNIDVRNARINEVMTRHPKTINADQLAAEAARLMEANKINGLIVVDPQQRAVG
ALNIHDLLHAKIV

Sequences:

>Translated_333_residues
MAVSLLQHNHLSDTALIASARRVIEIEREALTLLNERIGAPFVAACRLILNSHGRVISTGMGKSGHIARKIAATLASTGT
PAFFVHPGEAGHGDLGMITDSDVILALSYSGESDEVRMLLPVLKRQGNPIIAMTGRPQSTLAQAADVHLDVSVTTEACPL
DLAPTSSTTTSLVIGDALAVALLDARGFTAEDFARSHPAGHLGRRLLLHITDVMHSGDDLPAVHEEATLSEALLEMTRKR
LGMTAIVDNEGRLQGVFTDGDLRRALDSNIDVRNARINEVMTRHPKTINADQLAAEAARLMEANKINGLIVVDPQQRAVG
ALNIHDLLHAKIV
>Mature_332_residues
AVSLLQHNHLSDTALIASARRVIEIEREALTLLNERIGAPFVAACRLILNSHGRVISTGMGKSGHIARKIAATLASTGTP
AFFVHPGEAGHGDLGMITDSDVILALSYSGESDEVRMLLPVLKRQGNPIIAMTGRPQSTLAQAADVHLDVSVTTEACPLD
LAPTSSTTTSLVIGDALAVALLDARGFTAEDFARSHPAGHLGRRLLLHITDVMHSGDDLPAVHEEATLSEALLEMTRKRL
GMTAIVDNEGRLQGVFTDGDLRRALDSNIDVRNARINEVMTRHPKTINADQLAAEAARLMEANKINGLIVVDPQQRAVGA
LNIHDLLHAKIV

Specific function: Catalyzes the interconversion of D-arabinose 5-phosphate and D-ribulose 5-phosphate [H]

COG id: COG0794

COG function: function code M; Predicted sugar phosphate isomerase involved in capsule formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1789588, Length=315, Percent_Identity=56.5079365079365, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI87082151, Length=319, Percent_Identity=47.6489028213166, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR004800
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00571 CBS; PF01380 SIS [H]

EC number: =5.3.1.13 [H]

Molecular weight: Translated: 35587; Mature: 35456

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSLLQHNHLSDTALIASARRVIEIEREALTLLNERIGAPFVAACRLILNSHGRVISTG
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEC
MGKSGHIARKIAATLASTGTPAFFVHPGEAGHGDLGMITDSDVILALSYSGESDEVRMLL
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCEEEEEEECCCCCHHHHHH
PVLKRQGNPIIAMTGRPQSTLAQAADVHLDVSVTTEACPLDLAPTSSTTTSLVIGDALAV
HHHHCCCCCEEEECCCCHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHH
ALLDARGFTAEDFARSHPAGHLGRRLLLHITDVMHSGDDLPAVHEEATLSEALLEMTRKR
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LGMTAIVDNEGRLQGVFTDGDLRRALDSNIDVRNARINEVMTRHPKTINADQLAAEAARL
CCCEEEECCCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
MEANKINGLIVVDPQQRAVGALNIHDLLHAKIV
HHHHCCCCEEEECCCHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AVSLLQHNHLSDTALIASARRVIEIEREALTLLNERIGAPFVAACRLILNSHGRVISTG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEC
MGKSGHIARKIAATLASTGTPAFFVHPGEAGHGDLGMITDSDVILALSYSGESDEVRMLL
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCEEEEEEECCCCCHHHHHH
PVLKRQGNPIIAMTGRPQSTLAQAADVHLDVSVTTEACPLDLAPTSSTTTSLVIGDALAV
HHHHCCCCCEEEECCCCHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHH
ALLDARGFTAEDFARSHPAGHLGRRLLLHITDVMHSGDDLPAVHEEATLSEALLEMTRKR
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LGMTAIVDNEGRLQGVFTDGDLRRALDSNIDVRNARINEVMTRHPKTINADQLAAEAARL
CCCEEEECCCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
MEANKINGLIVVDPQQRAVGALNIHDLLHAKIV
HHHHCCCCEEEECCCHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]