Click here to switch to the map view.

The map label for this gene is rpoN [H]

Identifier: 28198545

GI number: 28198545

Start: 785517

End: 786905

Strand: Reverse

Name: rpoN [H]

Synonym: PD0637

Alternate gene names: 28198545

Gene position: 786905-785517 (Counterclockwise)

Preceding gene: 28198546

Following gene: 28198544

Centisome position: 31.23

GC content: 53.28

Gene sequence:

>1389_bases
ATGACATTGAAAGCAAGACTGCGAACATCCATCGGACAACAGCTGGTCATGACACCCCAGCTACACCAAAGCATCCGTCT
GCTGCAAATGTCCAACGCCGAATTGCAACTGGAAATCACCGCCGCGATAGAGAGCAATCCACTCTTGGACTGGGCCGAGA
ACCAATCATCGGAGACCAGCGAAGCTACCGAAAGGGAAGTAGAAACAGAAGATTGGCAAGCCAAAGAATCCGCCTGGGAT
ATCAACGGCAGTAAAAGCAACGAAAATGACGACAACAACCCTGCTGAACGTCTTACCACCGGGAACACTCTCACTGATCA
CTTGCTGTGGCAACTGCACCTCTCCCCACTGTCAGCACGTGATTACCAAATAGGCACCGTCTTGATTGATGCCATAGAAG
AAGATGGTTACCTGCGTGAACTTTTGTCCACACTGACTGCAACCCTGGCCCCGTTATCCGTAGATGAAAGCGAACTGCTA
CCAGTATTACGACACATTCAATCTTTTGACCCGAGCGGCGTGGCTGCACGCTCGCTGGCCGAGTGCTTGACACTGCAACT
GAAATCATTGAGAGAAGCGATACCCGGACGCACACTTGCACTGCAGATTGTCAACAGCCCACTGCTCGAACAATTGCCGC
GCAGTGGTGTCACCGGACTGGCACGCGAACTGAAACGACCCTCCCAAGACGTCCAACAGGCAGTGGAATTGATCCGTGGA
CTGGACCCTCGCCCAGGTAAGAAAATTGGCGACTTAGAAGTTGGAACCTACGTTGTACCGGACTGTGTGATCTGGCGCCA
ACATGGGTTGTGGCAAGCCGCGTTGTTGGAACGTGCATTCCCACAGGTCGTCCTCCACCGCGGCTACGAACAGCTCATCA
ACAGCTGCAATGACGCCAATGCGAGCTACCTACGCAACCAACTCCAGGAAGCACGGTGGCTGCTCAAGGGCCTGGAAGCA
CGCGGTGAAACCTTGTTAAAAGTGGTAAACAGCCTCATACGTCACCAAGCGGGATTCTTGGAATTCGGCCAACACGCGTT
ACGTCCATTGACTATCCGCGAATTAGCAACGGAACTTGCCCTGCATGAATCCACCGTATCCCGTGCAATTGCTGGCAAGT
ATGTACGCACCCCACGTGGCACCTTACCGCTACGCACCTTTTTCGCTTCAGGAATCAGCACCGACAGCGGCGGAGAGACC
TCCAGCTCTGCCATTCAAGCAATCATCAGACGTCTCATAGAAACTGAAAACCCCCGCAAGCCACTCTCTGACGCCAAACT
AGCTGAATTACTGAAGACATCCGGTATCCCAGTAGCGCGACGCACCATAGCAAAGTATCGTGATGCTATGAACATCTCCG
CATCCCACGAACGCGTCCGAGTCCTCTGA

Upstream 100 bases:

>100_bases
CACTGCTTACTAATACGGATGTGCGCCGCGTATACCTTGGAGAGACTTTCCACCTGTGATCGACATCTAACGCCGATGAT
GGTCAGTACGAATAAACGAT

Downstream 100 bases:

>100_bases
TCTATCTATTTCCGGCGTCTCAGCGCCAAACAGGTGGATCAAACAGGAATAGGATGATTGATTAACGTAATAACCAATGG
GAGGTATCCGATGCACATCG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTLKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKESAWD
INGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELL
PVLRHIQSFDPSGVAARSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRG
LDPRPGKKIGDLEVGTYVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLKGLEA
RGETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGET
SSSAIQAIIRRLIETENPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL

Sequences:

>Translated_462_residues
MTLKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKESAWD
INGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELL
PVLRHIQSFDPSGVAARSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRG
LDPRPGKKIGDLEVGTYVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLKGLEA
RGETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGET
SSSAIQAIIRRLIETENPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL
>Mature_461_residues
TLKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKESAWDI
NGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELLP
VLRHIQSFDPSGVAARSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGL
DPRPGKKIGDLEVGTYVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLKGLEAR
GETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGETS
SSAIQAIIRRLIETENPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen utilization genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=480, Percent_Identity=39.1666666666667, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51328; Mature: 51197

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETS
CCCHHHHHHHHCHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCHH
EATEREVETEDWQAKESAWDINGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSAR
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC
DYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELLPVLRHIQSFDPSGVAARSLA
CEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
ECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRG
HHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHC
LDPRPGKKIGDLEVGTYVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDAN
CCCCCCCCCCCCCCCCEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASYLRNQLQEARWLLKGLEARGETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGETSSSAIQAIIRRLIETENPRK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure 
TLKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETS
CCHHHHHHHHCHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCHH
EATEREVETEDWQAKESAWDINGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSAR
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC
DYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELLPVLRHIQSFDPSGVAARSLA
CEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
ECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRG
HHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHC
LDPRPGKKIGDLEVGTYVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDAN
CCCCCCCCCCCCCCCCEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASYLRNQLQEARWLLKGLEARGETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGETSSSAIQAIIRRLIETENPRK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969534 [H]