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The map label for this gene is ptsA [H]

Identifier: 28198539

GI number: 28198539

Start: 780209

End: 781936

Strand: Reverse

Name: ptsA [H]

Synonym: PD0631

Alternate gene names: 28198539

Gene position: 781936-780209 (Counterclockwise)

Preceding gene: 28198540

Following gene: 28198538

Centisome position: 31.03

GC content: 54.46

Gene sequence:

>1728_bases
ATGAATGTGCGCCTCCAGGGCCACGGCGCAGCACGAGGCACTGCACTGGGGCGCGCCTGGGTCCGTCAGATGAACATCAT
CGAAATCCAGGATAAATACATCACTGACACTAACGCCGAATTACATAGCTTGTACCAAGCCATGAGCGCAGCACGTCAGG
AAATACAAGTACTGCGAGAACGCATGCATGGCACCTTGTCCCACGAAGTCGGCGAGTTTCTCGACCTATACACACTGTTG
ATCGATGACCCTGAGCTACTACGGAGCCTGAACGAACTCATCCTCACTCACAACTACAGTGCCGAATACGCGCTGAAAAT
ACAGCGCGACCGCCTCACCACAGAATTCGAAGCCATGGAAGACCACTACCTGAAAAGTCGCATGGACGACCTGAATCACG
TGCTAGGACGCATCCACGCCTTTCTAAAAAAACACTCCCCCGAACCCAAAGATGCTGCAGGTGAAATCCTCATCTGCAAC
AACATCGCCCCATCCGAATTGGCCCAAGTACAAGCCCAAGGCATTATTGGAATCGTCATCGCGGCTGGCAATACACTCTC
GCACAGCGCCATCCTGGCGCATAGCTTGCACCTGCCACTGATCGTTGGTGCAGGAGAGCTGCTGCTACAAAAGATCACCA
ACGGCGACATGCTGATCATCGATGGCAACAGCGGAGAAATCATTGTCAATCCATCGCCACAGGACCTGCGTCACTACCAT
AGCCGACTGCGCGAACGCGCCAAAGAACAACGTGAGCTGGAACAACTACGCTCTAAACACAGCCGCACTCGCGACAACGT
CGACATTGTCGTACAGGCCAACGCAGAATCACCGGACGATGTCTCCCAAGCATACGCACTGGGCGCGGCCGGTGTGGGCC
TGTACCGCACTGAATTCCTGTTTCTGCAACGCAACAAACTGCCGGACGAAGAAGAACAGTTCCAAACATACCGCGACACT
GTACTACGTATGAACGGACGCCCAGTCACCATCCGCACATTAGATCTGGGTGCAGATAAAACCGACTGTACCGGCCTGAC
TCTCAGTAACGAAGAAAACCCAGCAATGGGCCTGCGTGGGATACGCCTGTCGATGGCACACCCCGCAGTGGCCAACACAC
AATTACGAGCGATCCTGCGCACCTCCACCTATGGGCATGTCCGTATCTTGGTCCCCATGGTTAGCTGCCGAGAAGAGATC
ATCCTTCTACGCAAGCAGATCAAAGCGCTCAGCATCCAATTGCGCACCGAAGGCCACGACATTGCCGAGCAGGTTCCACT
AGGAGCGATGATCGAAGTACCAGCTGCAGCAATCGCACTCAACAGCTTTATCAAAGAAATCGACTTCCTATCGATCGGTA
CCAACGACCTGGTCCAATACCTATTGGCCGCCGACCGCAACAATGCGGCAATCAGCGAAATATACTCCCCACTCCACCCT
GGCGTCCTGGCAGTACTGGCTCAGATCATCGTCACCGCACATACACATGCCACCCCTGTCGCCATCTGCGGTGAAATCGC
TGGCGATCCACATTACGTGCCCATGCTTCTGGCATTAGGTCTCACAGAATTCAGCCTTCACCCGGCCACATTACTTGAAG
TACGCCGTGCAATCCGCAACAGCGACCTGGGCACATTGAAAACAAGTGTAACCACACTGCTCCGCGCACGCGACCGGAAG
AGCATCGAAGAATGGATGGCGGCACACGCCAATACGACACCGACATAA

Upstream 100 bases:

>100_bases
GAGTGAACTGTTCGAAAACCGCTTCAACGAGGACACATAAAGAGCACAGATAAACATGCCCCACTCCGCTCATCGCCCCA
CCCCCACACTGCACACACAG

Downstream 100 bases:

>100_bases
TGCCACCATCGCTACCGGACGCTTATCTGGTCATCCTATTGAAAGACAATAATGAAGATTGATTGAATCCAACCGTACCC
TTTGGGAACGGTCCATTTTT

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MNVRLQGHGAARGTALGRAWVRQMNIIEIQDKYITDTNAELHSLYQAMSAARQEIQVLRERMHGTLSHEVGEFLDLYTLL
IDDPELLRSLNELILTHNYSAEYALKIQRDRLTTEFEAMEDHYLKSRMDDLNHVLGRIHAFLKKHSPEPKDAAGEILICN
NIAPSELAQVQAQGIIGIVIAAGNTLSHSAILAHSLHLPLIVGAGELLLQKITNGDMLIIDGNSGEIIVNPSPQDLRHYH
SRLRERAKEQRELEQLRSKHSRTRDNVDIVVQANAESPDDVSQAYALGAAGVGLYRTEFLFLQRNKLPDEEEQFQTYRDT
VLRMNGRPVTIRTLDLGADKTDCTGLTLSNEENPAMGLRGIRLSMAHPAVANTQLRAILRTSTYGHVRILVPMVSCREEI
ILLRKQIKALSIQLRTEGHDIAEQVPLGAMIEVPAAAIALNSFIKEIDFLSIGTNDLVQYLLAADRNNAAISEIYSPLHP
GVLAVLAQIIVTAHTHATPVAICGEIAGDPHYVPMLLALGLTEFSLHPATLLEVRRAIRNSDLGTLKTSVTTLLRARDRK
SIEEWMAAHANTTPT

Sequences:

>Translated_575_residues
MNVRLQGHGAARGTALGRAWVRQMNIIEIQDKYITDTNAELHSLYQAMSAARQEIQVLRERMHGTLSHEVGEFLDLYTLL
IDDPELLRSLNELILTHNYSAEYALKIQRDRLTTEFEAMEDHYLKSRMDDLNHVLGRIHAFLKKHSPEPKDAAGEILICN
NIAPSELAQVQAQGIIGIVIAAGNTLSHSAILAHSLHLPLIVGAGELLLQKITNGDMLIIDGNSGEIIVNPSPQDLRHYH
SRLRERAKEQRELEQLRSKHSRTRDNVDIVVQANAESPDDVSQAYALGAAGVGLYRTEFLFLQRNKLPDEEEQFQTYRDT
VLRMNGRPVTIRTLDLGADKTDCTGLTLSNEENPAMGLRGIRLSMAHPAVANTQLRAILRTSTYGHVRILVPMVSCREEI
ILLRKQIKALSIQLRTEGHDIAEQVPLGAMIEVPAAAIALNSFIKEIDFLSIGTNDLVQYLLAADRNNAAISEIYSPLHP
GVLAVLAQIIVTAHTHATPVAICGEIAGDPHYVPMLLALGLTEFSLHPATLLEVRRAIRNSDLGTLKTSVTTLLRARDRK
SIEEWMAAHANTTPT
>Mature_575_residues
MNVRLQGHGAARGTALGRAWVRQMNIIEIQDKYITDTNAELHSLYQAMSAARQEIQVLRERMHGTLSHEVGEFLDLYTLL
IDDPELLRSLNELILTHNYSAEYALKIQRDRLTTEFEAMEDHYLKSRMDDLNHVLGRIHAFLKKHSPEPKDAAGEILICN
NIAPSELAQVQAQGIIGIVIAAGNTLSHSAILAHSLHLPLIVGAGELLLQKITNGDMLIIDGNSGEIIVNPSPQDLRHYH
SRLRERAKEQRELEQLRSKHSRTRDNVDIVVQANAESPDDVSQAYALGAAGVGLYRTEFLFLQRNKLPDEEEQFQTYRDT
VLRMNGRPVTIRTLDLGADKTDCTGLTLSNEENPAMGLRGIRLSMAHPAVANTQLRAILRTSTYGHVRILVPMVSCREEI
ILLRKQIKALSIQLRTEGHDIAEQVPLGAMIEVPAAAIALNSFIKEIDFLSIGTNDLVQYLLAADRNNAAISEIYSPLHP
GVLAVLAQIIVTAHTHATPVAICGEIAGDPHYVPMLLALGLTEFSLHPATLLEVRRAIRNSDLGTLKTSVTTLLRARDRK
SIEEWMAAHANTTPT

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=549, Percent_Identity=34.7905282331512, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1789193, Length=549, Percent_Identity=32.0582877959927, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1788726, Length=539, Percent_Identity=30.4267161410019, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI48994992, Length=414, Percent_Identity=32.1256038647343, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1787994, Length=401, Percent_Identity=23.6907730673317, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI226510935, Length=193, Percent_Identity=28.4974093264249, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63772; Mature: 63772

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRLQGHGAARGTALGRAWVRQMNIIEIQDKYITDTNAELHSLYQAMSAARQEIQVLRE
CCEEEECCCCCCHHHHHHHHHHHCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
RMHGTLSHEVGEFLDLYTLLIDDPELLRSLNELILTHNYSAEYALKIQRDRLTTEFEAME
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCHHHHHHH
DHYLKSRMDDLNHVLGRIHAFLKKHSPEPKDAAGEILICNNIAPSELAQVQAQGIIGIVI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEE
AAGNTLSHSAILAHSLHLPLIVGAGELLLQKITNGDMLIIDGNSGEIIVNPSPQDLRHYH
ECCCCCCHHHHHHHHCCCCEEECCHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHH
SRLRERAKEQRELEQLRSKHSRTRDNVDIVVQANAESPDDVSQAYALGAAGVGLYRTEFL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHEE
FLQRNKLPDEEEQFQTYRDTVLRMNGRPVTIRTLDLGADKTDCTGLTLSNEENPAMGLRG
EEECCCCCCHHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCC
IRLSMAHPAVANTQLRAILRTSTYGHVRILVPMVSCREEIILLRKQIKALSIQLRTEGHD
EEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHEEEEECCCCH
IAEQVPLGAMIEVPAAAIALNSFIKEIDFLSIGTNDLVQYLLAADRNNAAISEIYSPLHP
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCHHHHHHHHCCCC
GVLAVLAQIIVTAHTHATPVAICGEIAGDPHYVPMLLALGLTEFSLHPATLLEVRRAIRN
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHCC
SDLGTLKTSVTTLLRARDRKSIEEWMAAHANTTPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNVRLQGHGAARGTALGRAWVRQMNIIEIQDKYITDTNAELHSLYQAMSAARQEIQVLRE
CCEEEECCCCCCHHHHHHHHHHHCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
RMHGTLSHEVGEFLDLYTLLIDDPELLRSLNELILTHNYSAEYALKIQRDRLTTEFEAME
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCHHHHHHH
DHYLKSRMDDLNHVLGRIHAFLKKHSPEPKDAAGEILICNNIAPSELAQVQAQGIIGIVI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEE
AAGNTLSHSAILAHSLHLPLIVGAGELLLQKITNGDMLIIDGNSGEIIVNPSPQDLRHYH
ECCCCCCHHHHHHHHCCCCEEECCHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHH
SRLRERAKEQRELEQLRSKHSRTRDNVDIVVQANAESPDDVSQAYALGAAGVGLYRTEFL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHEE
FLQRNKLPDEEEQFQTYRDTVLRMNGRPVTIRTLDLGADKTDCTGLTLSNEENPAMGLRG
EEECCCCCCHHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCC
IRLSMAHPAVANTQLRAILRTSTYGHVRILVPMVSCREEIILLRKQIKALSIQLRTEGHD
EEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHEEEEECCCCH
IAEQVPLGAMIEVPAAAIALNSFIKEIDFLSIGTNDLVQYLLAADRNNAAISEIYSPLHP
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCHHHHHHHHCCCC
GVLAVLAQIIVTAHTHATPVAICGEIAGDPHYVPMLLALGLTEFSLHPATLLEVRRAIRN
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHCC
SDLGTLKTSVTTLLRARDRKSIEEWMAAHANTTPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]