Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 28198441

GI number: 28198441

Start: 652582

End: 653271

Strand: Reverse

Name: lipB [H]

Synonym: PD0531

Alternate gene names: 28198441

Gene position: 653271-652582 (Counterclockwise)

Preceding gene: 28198442

Following gene: 28198440

Centisome position: 25.93

GC content: 58.26

Gene sequence:

>690_bases
GTGGACGCTGTGAATGCGTGTGGTGCTGTTGCGCCCTGGCATGTTGGCCGTGCCGCGCTGCTGCGTATGCTGGGTGCTCA
GCCTTATGTGCCGGTTTGGCATGCGATGCAACGTTTTACGGATGTACGCGATGCTACTGCGGTTGACGAGCTGTGGGTAG
TTGAGCATGAGCCGGTATTCACTCTTGGGCAGGCTGGGAAGCTTGAGCATGTCCTGGCCCCTGGGGAGATCCCGGTGGTG
CACGTGGACCGCGGTGGTCAGGTGACCTATCACGGTCCTGGTCAATTGGTGGTGTATCCACTGCTGGATCTATCCCGTCT
GGGGCTTGGTGTCCGTGATTATGTTTATGGGATTGAGCAGGCGGTGATTAATACGCTGGCTCAGTGGAATATTCTCGGTG
AGCGGCGCGAGCATGCTCCTGGGGTCTATGTCGGTGACGCCAAAATTGCCGCGCTGGGTATTCGGGTACGCCGTGGTTGC
AGTTTCCACGGTGTGGCGTTCAACGTTGCAATGGATTTGGAGCCGTTCCATCGTATTCATCCTTGTGGTTACCGGGGGCT
GCAGGTGACCTCGGTGTTAGACTTGGGGGGTCCTTCCGAGATGGACACGGTGGCGGCTGCATTGCTCGCTGAGCTAGCGC
GGCAATTTGGTTTCGTGCTGCACCCCACGTCCGGATGGCTTAGTTCTTAG

Upstream 100 bases:

>100_bases
CAACCGGGAAGTATGTGTCGGTGCGGATTGGCTTCCGTGCGCTGGATCGGGAGCAATATGACGCTGCTCACCAAGTGCTG
CGTGATCATCCGGAAGTGAA

Downstream 100 bases:

>100_bases
TTTTTTGCAAGCGGTCTAGCCTATTCTGGTATTGCGATGACTCAACCTATTATCCGTTCTATCCCGTTGCATGTTGTCTC
AAATGATCATCCTTCATCTT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MDAVNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLGQAGKLEHVLAPGEIPVV
HVDRGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGC
SFHGVAFNVAMDLEPFHRIHPCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS

Sequences:

>Translated_229_residues
MDAVNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLGQAGKLEHVLAPGEIPVV
HVDRGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGC
SFHGVAFNVAMDLEPFHRIHPCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS
>Mature_229_residues
MDAVNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLGQAGKLEHVLAPGEIPVV
HVDRGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGC
SFHGVAFNVAMDLEPFHRIHPCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=112, Percent_Identity=43.75, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI87081767, Length=193, Percent_Identity=56.4766839378238, Blast_Score=233, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6323268, Length=199, Percent_Identity=30.1507537688442, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21355483, Length=174, Percent_Identity=37.9310344827586, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24871; Mature: 24871

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEE
TLGQAGKLEHVLAPGEIPVVHVDRGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQ
EECCCCCEEEEECCCCEEEEEECCCCEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHH
AVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGCSFHGVAFNVAMDLEPFHRIH
HHHHHHHHHHHHCCHHHCCCCEEECCCEEEEEEEHHHCCCCCCCEEEEEEECCCHHHHCC
PCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCC
>Mature Secondary Structure
MDAVNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEE
TLGQAGKLEHVLAPGEIPVVHVDRGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQ
EECCCCCEEEEECCCCEEEEEECCCCEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHH
AVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGCSFHGVAFNVAMDLEPFHRIH
HHHHHHHHHHHHCCHHHCCCCEEECCCEEEEEEEHHHCCCCCCCEEEEEEECCCHHHHCC
PCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]