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The map label for this gene is metF [H]

Identifier: 28198329

GI number: 28198329

Start: 507938

End: 508765

Strand: Reverse

Name: metF [H]

Synonym: PD0413

Alternate gene names: 28198329

Gene position: 508765-507938 (Counterclockwise)

Preceding gene: 28198330

Following gene: 28198324

Centisome position: 20.19

GC content: 57.25

Gene sequence:

>828_bases
ATGATTCCAATCAGCTTCGAGTTTTATCCACCCAAAAACGACGATCAACGCGCACAGTTGGACAGGACAGCAAACCGGCT
ACGCGCATTCGCACCAGAATATGTCTCCTGCACCTTCGGCGCCGGTGGCTCCACACTCAGTTACACCTCAGAAACAGTGC
GCCATCTCAGCCAACACCACGGCTTTGACGCCGCACCGCATCTGTCCTGTGTGGGCGGCAGTCGCCAAGAAATCCGCGAA
CTTCTCAAACTGTACCGCGCGATTGGCTGCCAACGCATCGTGGCGCTACGCGGCGATCTCCCCTCGGGCATGGGCCACCC
CGGCGACCTCCGCTACGCAGCTGACCTGATTACCTTCATCCGTGCCGAGCATGGCGATCACTTCCACCTAGAGATCGGCG
CATACCCGGAAACCCACCCACAAGCCAGCAACACACTGAACGACCTTCACTATTTCAAAGCCAAAGTCGATGCAGGCGCC
GATGCGGCAATCACTCAATACTTTTACAACCCAGACGCCTATTTCCACTTCGTCGACGCAGTGCAGCGCCTGGGCGTCAC
CATCCCCATTGTTGCCGGAGTCATGCCCATCTCCAACTTTGACCAGTTGCGCCATTTCTCCGAACAATGCGGCGCCGAAA
TACCTCGCTGGATTACAAAAAAAATGCAGGCTTACGGCGACGACACCAAATCGATACGCGCGTTCGGTGCCGACGTCGTG
ACCGCACTATGTGAGCGGCTAATCGCTGGCGGCGCACCGGGGCTGCACTTCTACACGCTCAACCTAGCCAAACCAAGCAC
CCAAGTGCTGCAACGCTTAGGCTATTGA

Upstream 100 bases:

>100_bases
CACCGTCACGCCCCCTACCTCTAGCAGCAACAAAGACACCGAATTTCACATTCATCCGGATGAAGAGATAGAATAATCCA
GCCCCACCGATCACGTTGCC

Downstream 100 bases:

>100_bases
AGCCACCACAGCACGCCCACCACTGCATCACATACAGCAACAGTAGCCACGCCTTATTGCTTCGCTCCCCCCGGCCACAA
ACCACGCATGGCAGCAATCC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MIPISFEFYPPKNDDQRAQLDRTANRLRAFAPEYVSCTFGAGGSTLSYTSETVRHLSQHHGFDAAPHLSCVGGSRQEIRE
LLKLYRAIGCQRIVALRGDLPSGMGHPGDLRYAADLITFIRAEHGDHFHLEIGAYPETHPQASNTLNDLHYFKAKVDAGA
DAAITQYFYNPDAYFHFVDAVQRLGVTIPIVAGVMPISNFDQLRHFSEQCGAEIPRWITKKMQAYGDDTKSIRAFGADVV
TALCERLIAGGAPGLHFYTLNLAKPSTQVLQRLGY

Sequences:

>Translated_275_residues
MIPISFEFYPPKNDDQRAQLDRTANRLRAFAPEYVSCTFGAGGSTLSYTSETVRHLSQHHGFDAAPHLSCVGGSRQEIRE
LLKLYRAIGCQRIVALRGDLPSGMGHPGDLRYAADLITFIRAEHGDHFHLEIGAYPETHPQASNTLNDLHYFKAKVDAGA
DAAITQYFYNPDAYFHFVDAVQRLGVTIPIVAGVMPISNFDQLRHFSEQCGAEIPRWITKKMQAYGDDTKSIRAFGADVV
TALCERLIAGGAPGLHFYTLNLAKPSTQVLQRLGY
>Mature_275_residues
MIPISFEFYPPKNDDQRAQLDRTANRLRAFAPEYVSCTFGAGGSTLSYTSETVRHLSQHHGFDAAPHLSCVGGSRQEIRE
LLKLYRAIGCQRIVALRGDLPSGMGHPGDLRYAADLITFIRAEHGDHFHLEIGAYPETHPQASNTLNDLHYFKAKVDAGA
DAAITQYFYNPDAYFHFVDAVQRLGVTIPIVAGVMPISNFDQLRHFSEQCGAEIPRWITKKMQAYGDDTKSIRAFGADVV
TALCERLIAGGAPGLHFYTLNLAKPSTQVLQRLGY

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=278, Percent_Identity=36.3309352517986, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI1790377, Length=273, Percent_Identity=36.6300366300366, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI25147436, Length=277, Percent_Identity=30.6859205776173, Blast_Score=156, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI25147441, Length=277, Percent_Identity=30.6859205776173, Blast_Score=156, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6321313, Length=279, Percent_Identity=31.8996415770609, Blast_Score=155, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=286, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24662658, Length=224, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30487; Mature: 30487

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPISFEFYPPKNDDQRAQLDRTANRLRAFAPEYVSCTFGAGGSTLSYTSETVRHLSQHH
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GFDAAPHLSCVGGSRQEIRELLKLYRAIGCQRIVALRGDLPSGMGHPGDLRYAADLITFI
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
RAEHGDHFHLEIGAYPETHPQASNTLNDLHYFKAKVDAGADAAITQYFYNPDAYFHFVDA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHH
VQRLGVTIPIVAGVMPISNFDQLRHFSEQCGAEIPRWITKKMQAYGDDTKSIRAFGADVV
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TALCERLIAGGAPGLHFYTLNLAKPSTQVLQRLGY
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MIPISFEFYPPKNDDQRAQLDRTANRLRAFAPEYVSCTFGAGGSTLSYTSETVRHLSQHH
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GFDAAPHLSCVGGSRQEIRELLKLYRAIGCQRIVALRGDLPSGMGHPGDLRYAADLITFI
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
RAEHGDHFHLEIGAYPETHPQASNTLNDLHYFKAKVDAGADAAITQYFYNPDAYFHFVDA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHH
VQRLGVTIPIVAGVMPISNFDQLRHFSEQCGAEIPRWITKKMQAYGDDTKSIRAFGADVV
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TALCERLIAGGAPGLHFYTLNLAKPSTQVLQRLGY
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]