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The map label for this gene is cysS [H]

Identifier: 28198209

GI number: 28198209

Start: 364424

End: 365848

Strand: Reverse

Name: cysS [H]

Synonym: PD0287

Alternate gene names: 28198209

Gene position: 365848-364424 (Counterclockwise)

Preceding gene: 28198227

Following gene: 28198208

Centisome position: 14.52

GC content: 58.32

Gene sequence:

>1425_bases
TTGCTCCTAGGATGGTCTGTGATGAGCCTGCACCTTTACAACACCCTTCACCGGAGAGTCGAGCCGTTCGAACCGCTGGA
CCCTGCCTGCCCAACTATGTATGTATGCGGTCCGACCGTCTACAACTATGTGCATATCGGCAACGCCCGCGGTCCAGTCG
TGTTTGGGGTGTTGGCCGCGCTGCTGCGGCGGCGGTATGGGGCGCTGCGCTACGCACGCAACATCACTGACGTGGATGAC
AAGATCAACACTGCTGCGCAGGAGCAGGGTGTTCCCATCTCGGCCATTACTGAACGCTTTTGCGCTGCTTACCGTGAGGA
TATGAAAAAACTCGGTGTGGAGCCGCCTGATATCGAGCCGGAAGCCACTGCGCATATGCCACAGATCATCGCGATGATCG
AACAGCTGATCGAACGCCAGCATGCATATGTCGCGATGGAGCATGTGCTGTTCGCGGTGAACAGCTTTGCCGATTATGGG
CAACTGTCGCGCAGGGATACGGAAGAAATGTTGGCCGGTGCTCGAGTCGAGATCGCTCCATACAAGCGTGACCCAAGCGA
TTTCGTGCTGTGGAAACCTTCCAGCGATCAGCTTCCTGGCTGGGACTCGCCCTGGGGGCGCGGCCGCCCCGGCTGGCATA
TTGAGTGCTCCGCAATGGCCGCTGCGCATCTGGGGGAGACGATTGATATCCACGCTGGCGGAATCGATCTGCAATTCCCG
CATCATGAAAATGAAATCGCACAAAGTCGCTGCGCACACGGCAGCAGTACGTTCGCACGGGTCTGGATGCACAACGGGAT
GCTCAATTTTGAAGGTGCCAAAATGAGCAAATCGCTGGGCAATATCGAGACCGTGCATGAGTTGGTGGCAAAGCATCCTC
CCGAAGCACTACGTTACGCGCTGCTTTCGGCACATTATCGGAAACCGCTGGACTGGTCAGAGGCACTCATCGCACAGTCG
GTACGCACTCTAAACCGGCTGTATGGGACGCTGCGCGATTTGGCGGCGTATCCGGCACGCGCCATCATCCCAGGGAATAT
CGAAGCAGCGTTGGATGACGATCTCAACACGCCGCAGGCGCTGGCCGAGTTGGCGACACTGGCAAACGAGGCACGCATCC
AATTGGCTGACACCACACATCACGCAGCAGCCGAGGTCACTACCGCGCTCACCCGGCTGAAAGCCAAGTTGCTTGGCGCC
GGACTGGCTCTTGGTCTGCTGCAGCAAACGCCGGAGGCATGGTTCAGCCAAGGTACCAACGAGAGCGATGAAACGCATAT
CCAGGCGCTGATTGATGCACGCGGTGCCGCCAAGCAGGCGCGGGATTTTGTACGTGCCGACGCAATCCGTGCACAACTCG
CCGCAGAGGGTATTGTGCTTGAGGATACACCGCAGGGGGTACGCTGGATGAAGCAGCACACGTAA

Upstream 100 bases:

>100_bases
AATCTGTCAAGGGGCTGAACATACCCCTTCTGGCAGCCAATCACAGTCTCAAGTATGAACCAAAGCATCAAGATACGGTC
TCAGGCGCTAGAATCCGAGA

Downstream 100 bases:

>100_bases
AGGTGTGGATCTGACTATCTACGGCAATCACGCTGATACACTCGCCCTACCGTCACCGTACACGGAGTCATCCTGTTCAA
GACCATGACCGATGATCCAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLLGWSVMSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYGALRYARNITDVDD
KINTAAQEQGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYG
QLSRRDTEEMLAGARVEIAPYKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFP
HHENEIAQSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYRKPLDWSEALIAQS
VRTLNRLYGTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGA
GLALGLLQQTPEAWFSQGTNESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT

Sequences:

>Translated_474_residues
MLLGWSVMSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYGALRYARNITDVDD
KINTAAQEQGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYG
QLSRRDTEEMLAGARVEIAPYKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFP
HHENEIAQSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYRKPLDWSEALIAQS
VRTLNRLYGTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGA
GLALGLLQQTPEAWFSQGTNESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT
>Mature_474_residues
MLLGWSVMSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYGALRYARNITDVDD
KINTAAQEQGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYG
QLSRRDTEEMLAGARVEIAPYKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFP
HHENEIAQSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYRKPLDWSEALIAQS
VRTLNRLYGTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGA
GLALGLLQQTPEAWFSQGTNESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=388, Percent_Identity=36.5979381443299, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI21269877, Length=310, Percent_Identity=32.5806451612903, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI62240992, Length=310, Percent_Identity=32.5806451612903, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI10835051, Length=310, Percent_Identity=32.9032258064516, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI303304987, Length=310, Percent_Identity=32.5806451612903, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1786737, Length=470, Percent_Identity=45.7446808510638, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17509795, Length=295, Percent_Identity=32.2033898305085, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17509797, Length=295, Percent_Identity=32.2033898305085, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324082, Length=317, Percent_Identity=33.1230283911672, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19922218, Length=500, Percent_Identity=32.8, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI19922364, Length=277, Percent_Identity=38.9891696750903, Blast_Score=198, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 52459; Mature: 52459

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGWSVMSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAA
CEECHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHEEEEEECCCCCHHHHHHHHH
LLRRRYGALRYARNITDVDDKINTAAQEQGVPISAITERFCAAYREDMKKLGVEPPDIEP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYGQLSRRDTEEMLAGARVEIAP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEC
YKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFP
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCEEEECC
HHENEIAQSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYA
CCHHHHHHHHHCCCCHHHHHHHHHCCEEECCCHHHHHHHCCHHHHHHHHHHCCHHHHHHH
LLSAHYRKPLDWSEALIAQSVRTLNRLYGTLRDLAAYPARAIIPGNIEAALDDDLNTPQA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHH
LAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGAGLALGLLQQTPEAWFSQGTN
HHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
ESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure
MLLGWSVMSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAA
CEECHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHEEEEEECCCCCHHHHHHHHH
LLRRRYGALRYARNITDVDDKINTAAQEQGVPISAITERFCAAYREDMKKLGVEPPDIEP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYGQLSRRDTEEMLAGARVEIAP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEC
YKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFP
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCEEEECC
HHENEIAQSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYA
CCHHHHHHHHHCCCCHHHHHHHHHCCEEECCCHHHHHHHCCHHHHHHHHHHCCHHHHHHH
LLSAHYRKPLDWSEALIAQSVRTLNRLYGTLRDLAAYPARAIIPGNIEAALDDDLNTPQA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHH
LAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGAGLALGLLQQTPEAWFSQGTN
HHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
ESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]