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The map label for this gene is citN [H]

Identifier: 28198186

GI number: 28198186

Start: 334030

End: 335361

Strand: Reverse

Name: citN [H]

Synonym: PD0263

Alternate gene names: 28198186

Gene position: 335361-334030 (Counterclockwise)

Preceding gene: 28198187

Following gene: 28198185

Centisome position: 13.31

GC content: 54.05

Gene sequence:

>1332_bases
ATGCTGACTGCGCTCGGCTTCGGAATGGTCATCACCTTCATGTATTTGATAATGAGCAAGCGGCTTTCGCCGCTGGTCTC
ACTGATCATCGTGCCCATTGTGTTCGCATTGGCTGGCGGCTTCGGCTCCAGCATGAGCGAAATGATGCTTGAAGGGATCA
AAAAGATTGCGCCAACCGGCGTGATGCTGATGTTCGCCATTCTTTACTTCGGGGTGATGATTGATGCTGGCCTGTTCGAT
CCGCTCGTACGGCGCATTCTGCGGATAGTCAAAGGCGATCCGATGAAGATCGTTATGGGTACGGCAATCCTGGCGCTACT
GATCTCACTCGACGGTGACGGTTCGACAACCTACATGATCACCGTCTCGGCAATGCTACCGCTGTACCAGCGTCTGGGCC
TAAACGCACTGAATCTGACTTGCGTGACCATCCTGGCCGGCGGAGTGATGAACCTGACACCATGGGGCGGCCCAACCGCA
CGCGCGGCGACCGCGCTACGTGTCGATCCAGCCGATGTCTTCATCCCCCTCATCCCGGCGATGGGCATCGCCATCATCGG
CATTCTGACGCTGGCATGGTGGCTAGGCATGAAGGAACGCAAACGTCTGGGTGTGGCCAAATTGCCTAACAGCGGCGCTT
GGAATGGTTACGCAATGCACGATGATGAAGATGCGCTGCCCACCATGGAAGGCGATGCCGAAGATATCAAGCGACCCAAG
CTGCTGTGGGTGAATTTTGCGCTCACTATCGCCCTGATGGCCGGATTAGTCGTTGGCATACTTCCGATGCCCATCCTGTT
CATGATCGGCTTCGCATTAGCGCTCCTGATTAATTATCCAAACTTGGCAGAACAACGCCGTCGTGTGGTTAATCATGCAG
GGAACGTTCTTTCAGTGGTCTCGTTGATCTTTGCTGCCGGCATCTTCACAGGCATCTTGTCAAACACTGGAATGGTCGAC
GCGATGTCACGCAGCTTTCTGGCACTCATACCAGACAGCTTGGGGCCCTACTTGGCGGTGATTACTGCTATCGCCAGCAT
GCCCTTCACTTTTTTTATGTCCAACGATGCGTTCTATTTCGGTGTGCTCCCGATCCTATCCGAAGCCGCCAGCCACTACG
GCATTACCCCTGTAGAAATGGCACGTGCCTCATTGGCTGGGCAACCGGTACATCTACTGAGTCCACTCGTTCCTTCCACC
TACCTACTGGTCGGACTTGCCAAAGTAGATTTCGGTGACCACCAGCGTTTTTCCCTCAAATGGGCGGTGTTAATCTCAAT
GGTGCTATTACTCAGTAGCCTATTGTTTTTGATTTACCCACTGGCCGCATGA

Upstream 100 bases:

>100_bases
TATTGACTTTCAGCCAAGTGCTACGCCGCAATAGGTGCGGCGTATGCGCGATAACGGCATGCTATCGCCTCCCACCTGCA
ACATTCCAAGGAAACTCTCC

Downstream 100 bases:

>100_bases
ACTTGACTTCTCATCTAATCTCCACACGCCCACTTTTAATTTCTACAAGGAGTCTCGTATGACCCTTCGCATCGCCTACG
TCACCAGCGGCATGGGCAGT

Product: Mg++/citrate complex transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLTALGFGMVITFMYLIMSKRLSPLVSLIIVPIVFALAGGFGSSMSEMMLEGIKKIAPTGVMLMFAILYFGVMIDAGLFD
PLVRRILRIVKGDPMKIVMGTAILALLISLDGDGSTTYMITVSAMLPLYQRLGLNALNLTCVTILAGGVMNLTPWGGPTA
RAATALRVDPADVFIPLIPAMGIAIIGILTLAWWLGMKERKRLGVAKLPNSGAWNGYAMHDDEDALPTMEGDAEDIKRPK
LLWVNFALTIALMAGLVVGILPMPILFMIGFALALLINYPNLAEQRRRVVNHAGNVLSVVSLIFAAGIFTGILSNTGMVD
AMSRSFLALIPDSLGPYLAVITAIASMPFTFFMSNDAFYFGVLPILSEAASHYGITPVEMARASLAGQPVHLLSPLVPST
YLLVGLAKVDFGDHQRFSLKWAVLISMVLLLSSLLFLIYPLAA

Sequences:

>Translated_443_residues
MLTALGFGMVITFMYLIMSKRLSPLVSLIIVPIVFALAGGFGSSMSEMMLEGIKKIAPTGVMLMFAILYFGVMIDAGLFD
PLVRRILRIVKGDPMKIVMGTAILALLISLDGDGSTTYMITVSAMLPLYQRLGLNALNLTCVTILAGGVMNLTPWGGPTA
RAATALRVDPADVFIPLIPAMGIAIIGILTLAWWLGMKERKRLGVAKLPNSGAWNGYAMHDDEDALPTMEGDAEDIKRPK
LLWVNFALTIALMAGLVVGILPMPILFMIGFALALLINYPNLAEQRRRVVNHAGNVLSVVSLIFAAGIFTGILSNTGMVD
AMSRSFLALIPDSLGPYLAVITAIASMPFTFFMSNDAFYFGVLPILSEAASHYGITPVEMARASLAGQPVHLLSPLVPST
YLLVGLAKVDFGDHQRFSLKWAVLISMVLLLSSLLFLIYPLAA
>Mature_443_residues
MLTALGFGMVITFMYLIMSKRLSPLVSLIIVPIVFALAGGFGSSMSEMMLEGIKKIAPTGVMLMFAILYFGVMIDAGLFD
PLVRRILRIVKGDPMKIVMGTAILALLISLDGDGSTTYMITVSAMLPLYQRLGLNALNLTCVTILAGGVMNLTPWGGPTA
RAATALRVDPADVFIPLIPAMGIAIIGILTLAWWLGMKERKRLGVAKLPNSGAWNGYAMHDDEDALPTMEGDAEDIKRPK
LLWVNFALTIALMAGLVVGILPMPILFMIGFALALLINYPNLAEQRRRVVNHAGNVLSVVSLIFAAGIFTGILSNTGMVD
AMSRSFLALIPDSLGPYLAVITAIASMPFTFFMSNDAFYFGVLPILSEAASHYGITPVEMARASLAGQPVHLLSPLVPST
YLLVGLAKVDFGDHQRFSLKWAVLISMVLLLSSLLFLIYPLAA

Specific function: Transports the free citrate anion. Probably cotransports citrate and at least three or four protons. The citrate uptake is inhibited by the presence of magnesium ions [H]

COG id: COG2851

COG function: function code C; H+/citrate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014738
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 47627; Mature: 47627

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTALGFGMVITFMYLIMSKRLSPLVSLIIVPIVFALAGGFGSSMSEMMLEGIKKIAPTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
VMLMFAILYFGVMIDAGLFDPLVRRILRIVKGDPMKIVMGTAILALLISLDGDGSTTYMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
TVSAMLPLYQRLGLNALNLTCVTILAGGVMNLTPWGGPTARAATALRVDPADVFIPLIPA
EHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEECCHHHHHHHHHHH
MGIAIIGILTLAWWLGMKERKRLGVAKLPNSGAWNGYAMHDDEDALPTMEGDAEDIKRPK
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
LLWVNFALTIALMAGLVVGILPMPILFMIGFALALLINYPNLAEQRRRVVNHAGNVLSVV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
SLIFAAGIFTGILSNTGMVDAMSRSFLALIPDSLGPYLAVITAIASMPFTFFMSNDAFYF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCHHH
GVLPILSEAASHYGITPVEMARASLAGQPVHLLSPLVPSTYLLVGLAKVDFGDHQRFSLK
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
WAVLISMVLLLSSLLFLIYPLAA
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTALGFGMVITFMYLIMSKRLSPLVSLIIVPIVFALAGGFGSSMSEMMLEGIKKIAPTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
VMLMFAILYFGVMIDAGLFDPLVRRILRIVKGDPMKIVMGTAILALLISLDGDGSTTYMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
TVSAMLPLYQRLGLNALNLTCVTILAGGVMNLTPWGGPTARAATALRVDPADVFIPLIPA
EHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEECCHHHHHHHHHHH
MGIAIIGILTLAWWLGMKERKRLGVAKLPNSGAWNGYAMHDDEDALPTMEGDAEDIKRPK
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
LLWVNFALTIALMAGLVVGILPMPILFMIGFALALLINYPNLAEQRRRVVNHAGNVLSVV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
SLIFAAGIFTGILSNTGMVDAMSRSFLALIPDSLGPYLAVITAIASMPFTFFMSNDAFYF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCHHH
GVLPILSEAASHYGITPVEMARASLAGQPVHLLSPLVPSTYLLVGLAKVDFGDHQRFSLK
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
WAVLISMVLLLSSLLFLIYPLAA
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377; 7704256; 8892821 [H]