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The map label for this gene is mazG [H]

Identifier: 28198083

GI number: 28198083

Start: 194491

End: 195324

Strand: Reverse

Name: mazG [H]

Synonym: PD0152

Alternate gene names: 28198083

Gene position: 195324-194491 (Counterclockwise)

Preceding gene: 77747634

Following gene: 28198082

Centisome position: 7.75

GC content: 52.88

Gene sequence:

>834_bases
ATGTCCGATCTTCCCGACGCATCACCCCACATTGAAACGTTGCTATCCATCATGGCGCGCCTGCGCGACCCGATCAGGGG
ATGCCCTTGGAATTTAGAGAAAGACTTTACCACGATTGCGCCTTACACGATTGAAGAGGCTTATGAAGTTGTCGACGCGA
TCGACCGCAACGACCTAAACGCCCTGAAAGACGAACTTGGAGACCTGCTGCTACAAATCATTTTCCATTCGCATATGGCC
GCCGAACAGGGTGCATTCGAATTTACCGACGTAATTACCGCCATTACCGACAAAATGGTACGCAGGCATCCCCACGTGTT
CGGTGACATCACCGTAGACGGTGCAGAGACAGTCAGTACTCACTGGGAGGCCATCAAGCGCCAAGAACGCGAAGCTGTTA
ACGAGCAAGATCATTCTGCACTGTTCGGGATTTCAAGCGGGCTACCAGAATGGCTACGCGCCCTCAAGTTGCAGGAGCGC
GCCACGCGCGCTGGTTTCAAGCAAGCAGCCCCAATCCCTGCCATCGTCGAGATGCAGAAGACACTGGAACAGATCCGCCA
AACGTTTCTAATGGACACCGTGACACACAACAAAGCACAGATTGAGGAGATATTCGGCAATCTGCTGTTCATCTGCATCC
AGCTTGCCCACCACGCCAAGGTAGATCCAGGCAACGCGCTACGTCATACCAACATTCAATTTGAAAAACAGTTCCGCGCC
ATGGAAAAAACCGCGCAGGCTGCAGGCGCTACGCTGGCACAAATGTCACTCTCAGAACAGCAGACCCTGTGGAACACTGT
TAACCGGCAAGAAACCGATCCATCAGCACCATAA

Upstream 100 bases:

>100_bases
ACTGCTTAACGGCGGTTTTATCGCACTGGGCGACGCCACGCTGCCTTGGCGCGATTGGTTGCCCTGACTCCATCTTATTC
CCCATTCTGGCGCTTTCCGT

Downstream 100 bases:

>100_bases
GAACGACGCTTCTGTAAAACACTGGCACTGATACGCCACCACACATCGCACTGCATTCTATTGGGTCGCTGCCAGATCAA
ACGTTGCAATGGCTTTAGTG

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSDLPDASPHIETLLSIMARLRDPIRGCPWNLEKDFTTIAPYTIEEAYEVVDAIDRNDLNALKDELGDLLLQIIFHSHMA
AEQGAFEFTDVITAITDKMVRRHPHVFGDITVDGAETVSTHWEAIKRQEREAVNEQDHSALFGISSGLPEWLRALKLQER
ATRAGFKQAAPIPAIVEMQKTLEQIRQTFLMDTVTHNKAQIEEIFGNLLFICIQLAHHAKVDPGNALRHTNIQFEKQFRA
MEKTAQAAGATLAQMSLSEQQTLWNTVNRQETDPSAP

Sequences:

>Translated_277_residues
MSDLPDASPHIETLLSIMARLRDPIRGCPWNLEKDFTTIAPYTIEEAYEVVDAIDRNDLNALKDELGDLLLQIIFHSHMA
AEQGAFEFTDVITAITDKMVRRHPHVFGDITVDGAETVSTHWEAIKRQEREAVNEQDHSALFGISSGLPEWLRALKLQER
ATRAGFKQAAPIPAIVEMQKTLEQIRQTFLMDTVTHNKAQIEEIFGNLLFICIQLAHHAKVDPGNALRHTNIQFEKQFRA
MEKTAQAAGATLAQMSLSEQQTLWNTVNRQETDPSAP
>Mature_276_residues
SDLPDASPHIETLLSIMARLRDPIRGCPWNLEKDFTTIAPYTIEEAYEVVDAIDRNDLNALKDELGDLLLQIIFHSHMAA
EQGAFEFTDVITAITDKMVRRHPHVFGDITVDGAETVSTHWEAIKRQEREAVNEQDHSALFGISSGLPEWLRALKLQERA
TRAGFKQAAPIPAIVEMQKTLEQIRQTFLMDTVTHNKAQIEEIFGNLLFICIQLAHHAKVDPGNALRHTNIQFEKQFRAM
EKTAQAAGATLAQMSLSEQQTLWNTVNRQETDPSAP

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=264, Percent_Identity=41.2878787878788, Blast_Score=220, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 31219; Mature: 31088

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLPDASPHIETLLSIMARLRDPIRGCPWNLEKDFTTIAPYTIEEAYEVVDAIDRNDLN
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCHHH
ALKDELGDLLLQIIFHSHMAAEQGAFEFTDVITAITDKMVRRHPHVFGDITVDGAETVST
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
HWEAIKRQEREAVNEQDHSALFGISSGLPEWLRALKLQERATRAGFKQAAPIPAIVEMQK
HHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHH
TLEQIRQTFLMDTVTHNKAQIEEIFGNLLFICIQLAHHAKVDPGNALRHTNIQFEKQFRA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
MEKTAQAAGATLAQMSLSEQQTLWNTVNRQETDPSAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDLPDASPHIETLLSIMARLRDPIRGCPWNLEKDFTTIAPYTIEEAYEVVDAIDRNDLN
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCHHH
ALKDELGDLLLQIIFHSHMAAEQGAFEFTDVITAITDKMVRRHPHVFGDITVDGAETVST
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
HWEAIKRQEREAVNEQDHSALFGISSGLPEWLRALKLQERATRAGFKQAAPIPAIVEMQK
HHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHH
TLEQIRQTFLMDTVTHNKAQIEEIFGNLLFICIQLAHHAKVDPGNALRHTNIQFEKQFRA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
MEKTAQAAGATLAQMSLSEQQTLWNTVNRQETDPSAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]