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The map label for this gene is pyrE [H]

Identifier: 28198058

GI number: 28198058

Start: 155324

End: 155983

Strand: Reverse

Name: pyrE [H]

Synonym: PD0122

Alternate gene names: 28198058

Gene position: 155983-155324 (Counterclockwise)

Preceding gene: 28198059

Following gene: 28198057

Centisome position: 6.19

GC content: 52.73

Gene sequence:

>660_bases
ATGTCTCACTACCGCCAACGTTTCCTACAGCTGGCTTTAGACTCAAACGCCCTGTGCTTCGGCGAATTCACGCTCAAATC
CGGCCGCATCAGTCCCTATTTCTTCAACGCTGGACACTTTAATTCAGGTGCAAAAACTGCTGCCTTAGCACAGTGTTACG
CCGACGCAATTGATGCGGCGAATATGAACTTTGACCTGGTGTTTGGTCCAGCCTACAAAGGGATTCCACTCGCTACAGCA
CTGGCCTGCGAGTACGCACAGCGTGAGCGCGACCTACTCCTCACATTCAATCGTAAAGAAGTCAAAAATCACGGCGAGGG
TGGCACTCTCATCGGTGCACCACTGAACGGACGCAAGATATTGATTATTGATGATGTCATCACCGCAGGCACAGCAATCC
GCGAAGTATTACGTATCATCCGCAACGCTGGAGGTACCCCAACGGGGGTTGCTGTAGCCCTGAATCGCCAGGAAATCGCT
TCAGAGACCAACCGCCAATCGTCCGTTCAAGCACTGATGGCCGAAACCGGGATCCCTGTCGTAGCGATCGCAACCCTGAA
CGACCTGTTAGCATTTGTAGAAGAGAACGCCAGTTTGGCCAAGTTTTACGAGCCTCTGCTGGCCTACAAGACCCACTATG
GGACCGAAGCACCTGACTGA

Upstream 100 bases:

>100_bases
AGAACACCATCCGAAAAGTCCCTCTTGGCACGATAGAGAAGACCGCGCCAGTGGCTATCCTTACAGCTTTATCCTCTCCA
CATGACTTAGTGACCCCTCT

Downstream 100 bases:

>100_bases
ACAAGCTAAAAAGTGACCTGCGATGTCCATAAAAAAACACTACATCTACTGGAACACCATCTTATTTGTTGCCGTTGCCG
CATGGCCGTGGCCTGGCAAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAANMNFDLVFGPAYKGIPLATA
LACEYAQRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKILIIDDVITAGTAIREVLRIIRNAGGTPTGVAVALNRQEIA
SETNRQSSVQALMAETGIPVVAIATLNDLLAFVEENASLAKFYEPLLAYKTHYGTEAPD

Sequences:

>Translated_219_residues
MSHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAANMNFDLVFGPAYKGIPLATA
LACEYAQRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKILIIDDVITAGTAIREVLRIIRNAGGTPTGVAVALNRQEIA
SETNRQSSVQALMAETGIPVVAIATLNDLLAFVEENASLAKFYEPLLAYKTHYGTEAPD
>Mature_218_residues
SHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAANMNFDLVFGPAYKGIPLATAL
ACEYAQRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKILIIDDVITAGTAIREVLRIIRNAGGTPTGVAVALNRQEIAS
ETNRQSSVQALMAETGIPVVAIATLNDLLAFVEENASLAKFYEPLLAYKTHYGTEAPD

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=187, Percent_Identity=28.8770053475936, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1790073, Length=216, Percent_Identity=47.6851851851852, Blast_Score=200, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17508631, Length=149, Percent_Identity=33.5570469798658, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=155, Percent_Identity=24.5161290322581, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=200, Percent_Identity=46.5, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323927, Length=199, Percent_Identity=46.2311557788945, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23700; Mature: 23569

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAA
CCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
NMNFDLVFGPAYKGIPLATALACEYAQRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKI
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCEEEECCCCCCEE
LIIDDVITAGTAIREVLRIIRNAGGTPTGVAVALNRQEIASETNRQSSVQALMAETGIPV
EEECCHHHCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCHHHHHHHHHHHCCCCE
VAIATLNDLLAFVEENASLAKFYEPLLAYKTHYGTEAPD
EEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAA
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
NMNFDLVFGPAYKGIPLATALACEYAQRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKI
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCEEEECCCCCCEE
LIIDDVITAGTAIREVLRIIRNAGGTPTGVAVALNRQEIASETNRQSSVQALMAETGIPV
EEECCHHHCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCHHHHHHHHHHHCCCCE
VAIATLNDLLAFVEENASLAKFYEPLLAYKTHYGTEAPD
EEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA