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The map label for this gene is pbp [H]

Identifier: 27366462

GI number: 27366462

Start: 3280318

End: 3281826

Strand: Reverse

Name: pbp [H]

Synonym: VV1_3205

Alternate gene names: 27366462

Gene position: 3281826-3280318 (Counterclockwise)

Preceding gene: NA

Following gene: 27366458

Centisome position: 100.0

GC content: 50.17

Gene sequence:

>1509_bases
ATGGATCAAGCCAATAAGAAGAAAATGAAATTATTACCTACCGTAATAGTTTCGTCTTTATTGACAATGCAGCCGGCATT
GGCACATCAAAATAATAAAATGACACAGGAAGAAGGCCAAGCAACCTTCAATGCCGTCGTTGCCGATGTCAATAAAATGA
TCAAAGAAATGCACATACCGGGACTTGCTGTCGGTGTTATTCATGGACCTAACACCTACAGTGCCGGACTCGGTATCACC
AAGGTGGGCACCAACGAGGCCGTTACCCCTGATACACAATTTCAAATTGGCTCTGTGACCAAAACCTTCCTTGCGACCCT
AGCCATGCAGCAATCCGAACAGGGCATTCTGGATTTAAACGCACGTGTCGTCGATATTCTGCCGTGGTGGAGGGTTTCAG
ATCCCGAGGCGACATCTAAAGCGCGGGTTGTTGACCTATTCCACCATCGTGCTGGCTGGTATGGCGACCATGGGTTCCTT
CGTGTTCCTCCAGGTGGTTCGATATCTGAGAAAGTCATGCTTCAAGGGGCTTACGCCCAGCAGATCTATCCGTTCGATCA
AACCTGGATGTACAACAACACCAGTATCACTTTCGCTACACATGTAGTGTCTCATGTCGGTGGAAAGCCGATCGAGACAC
TGATCGAAGAGAACCTGTTAACTCCGCTCGGCATGAATTCGTCATCCTTTAATTACGATGCTACCCCTCCTGCTAAAGAT
TACGCATGGGGCCATCCGCCGATCTACGGGAAAGGCAGCATCAGTGAACTCAAGCCGTACTACATTCCGTTGATTAGAGA
GTCCGCAGCCTCCGGTGCACTCCGCTCTACGGTGGCCGACATGCTTAAATACGCCCGCTTTCAGCTAGACGGTAAGGATG
CCTCCGGTAAGCAGTTGTTGTCTAAAAAAGCGCTGGACTATGCCCATGCACCTGCAGTCGAGGCTTCGACCGGAGACTTT
ACCGGACTGACATGGTTCGTCCACGACTACGATGGGACCACAAGCCCTAGCCATGGGGGGAATTGGCCCGGTACGCGTTC
TTTTCTGCAGTTGATCCCGGATCACGACTTTGCCGTAGTGGTTTTGGTTCACAGCGATCGCGGAGCTGAAGCGTACAAAA
AAGTGGCTAACGCAATGGTCAAAGCCTTTACGAAGATCGAATCCAACTCTCCGGTCTCCGTTGGTGCGACTCCGTCTGTG
CTCGACCCCTTCGTCGGCGTGTTTAAGGGTAATTCCCAAAACATTGAGATCCGTAAAGATGGCGACAAATATGTGTTTGT
CCAGTTTTCAGCATTGACTAAAGGAGCGGATCCTGCCCCTGCCAATGTAGAAAACACCGCCGAGGGTTATTTTATCATCA
CCGAAGGTCCGAATAAGGGAGCGCTTGGTGAATTGCTGAAAGACCCATCTGGCGAACTCAACTACGTGCGCTTCAACCAC
AGAATCTTTATTCGCGGTGAAGGTGCGGTTAACGAGTTCGACCTATCTGGATCGGTGTTCGATAAGTAG

Upstream 100 bases:

>100_bases
CGTGATCTAGCTATTGCAGAAATTACCCGAACGTTATACGATTTTTATTATTTAAAGTAACGAATTAAATTCAACTGTTA
GTGTTTAAAAAGGTATAAAT

Downstream 100 bases:

>100_bases
GATGAGCAAAAGATATCAATTCACGGGAATAATAGTTTCTGGTAGATATCTATCGGCAGTATGAATATCCTTAAACATCA
AAACACGCCATATGGACATG

Product: beta-lactamase class C

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP-4 [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MDQANKKKMKLLPTVIVSSLLTMQPALAHQNNKMTQEEGQATFNAVVADVNKMIKEMHIPGLAVGVIHGPNTYSAGLGIT
KVGTNEAVTPDTQFQIGSVTKTFLATLAMQQSEQGILDLNARVVDILPWWRVSDPEATSKARVVDLFHHRAGWYGDHGFL
RVPPGGSISEKVMLQGAYAQQIYPFDQTWMYNNTSITFATHVVSHVGGKPIETLIEENLLTPLGMNSSSFNYDATPPAKD
YAWGHPPIYGKGSISELKPYYIPLIRESAASGALRSTVADMLKYARFQLDGKDASGKQLLSKKALDYAHAPAVEASTGDF
TGLTWFVHDYDGTTSPSHGGNWPGTRSFLQLIPDHDFAVVVLVHSDRGAEAYKKVANAMVKAFTKIESNSPVSVGATPSV
LDPFVGVFKGNSQNIEIRKDGDKYVFVQFSALTKGADPAPANVENTAEGYFIITEGPNKGALGELLKDPSGELNYVRFNH
RIFIRGEGAVNEFDLSGSVFDK

Sequences:

>Translated_502_residues
MDQANKKKMKLLPTVIVSSLLTMQPALAHQNNKMTQEEGQATFNAVVADVNKMIKEMHIPGLAVGVIHGPNTYSAGLGIT
KVGTNEAVTPDTQFQIGSVTKTFLATLAMQQSEQGILDLNARVVDILPWWRVSDPEATSKARVVDLFHHRAGWYGDHGFL
RVPPGGSISEKVMLQGAYAQQIYPFDQTWMYNNTSITFATHVVSHVGGKPIETLIEENLLTPLGMNSSSFNYDATPPAKD
YAWGHPPIYGKGSISELKPYYIPLIRESAASGALRSTVADMLKYARFQLDGKDASGKQLLSKKALDYAHAPAVEASTGDF
TGLTWFVHDYDGTTSPSHGGNWPGTRSFLQLIPDHDFAVVVLVHSDRGAEAYKKVANAMVKAFTKIESNSPVSVGATPSV
LDPFVGVFKGNSQNIEIRKDGDKYVFVQFSALTKGADPAPANVENTAEGYFIITEGPNKGALGELLKDPSGELNYVRFNH
RIFIRGEGAVNEFDLSGSVFDK
>Mature_502_residues
MDQANKKKMKLLPTVIVSSLLTMQPALAHQNNKMTQEEGQATFNAVVADVNKMIKEMHIPGLAVGVIHGPNTYSAGLGIT
KVGTNEAVTPDTQFQIGSVTKTFLATLAMQQSEQGILDLNARVVDILPWWRVSDPEATSKARVVDLFHHRAGWYGDHGFL
RVPPGGSISEKVMLQGAYAQQIYPFDQTWMYNNTSITFATHVVSHVGGKPIETLIEENLLTPLGMNSSSFNYDATPPAKD
YAWGHPPIYGKGSISELKPYYIPLIRESAASGALRSTVADMLKYARFQLDGKDASGKQLLSKKALDYAHAPAVEASTGDF
TGLTWFVHDYDGTTSPSHGGNWPGTRSFLQLIPDHDFAVVVLVHSDRGAEAYKKVANAMVKAFTKIESNSPVSVGATPSV
LDPFVGVFKGNSQNIEIRKDGDKYVFVQFSALTKGADPAPANVENTAEGYFIITEGPNKGALGELLKDPSGELNYVRFNH
RIFIRGEGAVNEFDLSGSVFDK

Specific function: Involved in cell wall biosynthesis and may also act as a sensor of external penicillins [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=284, Percent_Identity=25.7042253521127, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 54611; Mature: 54611

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQANKKKMKLLPTVIVSSLLTMQPALAHQNNKMTQEEGQATFNAVVADVNKMIKEMHIP
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GLAVGVIHGPNTYSAGLGITKVGTNEAVTPDTQFQIGSVTKTFLATLAMQQSEQGILDLN
CEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEECC
ARVVDILPWWRVSDPEATSKARVVDLFHHRAGWYGDHGFLRVPPGGSISEKVMLQGAYAQ
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEECCCCEEECCCCCCCCHHHEEECCHHH
QIYPFDQTWMYNNTSITFATHVVSHVGGKPIETLIEENLLTPLGMNSSSFNYDATPPAKD
HCCCCCCEEEECCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
YAWGHPPIYGKGSISELKPYYIPLIRESAASGALRSTVADMLKYARFQLDGKDASGKQLL
CCCCCCCCCCCCCHHHCCCEEEHEEHHHHHCCHHHHHHHHHHHHHHEEECCCCCCHHHHH
SKKALDYAHAPAVEASTGDFTGLTWFVHDYDGTTSPSHGGNWPGTRSFLQLIPDHDFAVV
HHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEE
VLVHSDRGAEAYKKVANAMVKAFTKIESNSPVSVGATPSVLDPFVGVFKGNSQNIEIRKD
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCCCEEEEEEC
GDKYVFVQFSALTKGADPAPANVENTAEGYFIITEGPNKGALGELLKDPSGELNYVRFNH
CCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCEEEEEEEE
RIFIRGEGAVNEFDLSGSVFDK
EEEEEECCCCCEEECCCCCCCC
>Mature Secondary Structure
MDQANKKKMKLLPTVIVSSLLTMQPALAHQNNKMTQEEGQATFNAVVADVNKMIKEMHIP
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GLAVGVIHGPNTYSAGLGITKVGTNEAVTPDTQFQIGSVTKTFLATLAMQQSEQGILDLN
CEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEECC
ARVVDILPWWRVSDPEATSKARVVDLFHHRAGWYGDHGFLRVPPGGSISEKVMLQGAYAQ
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEECCCCEEECCCCCCCCHHHEEECCHHH
QIYPFDQTWMYNNTSITFATHVVSHVGGKPIETLIEENLLTPLGMNSSSFNYDATPPAKD
HCCCCCCEEEECCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
YAWGHPPIYGKGSISELKPYYIPLIRESAASGALRSTVADMLKYARFQLDGKDASGKQLL
CCCCCCCCCCCCCHHHCCCEEEHEEHHHHHCCHHHHHHHHHHHHHHEEECCCCCCHHHHH
SKKALDYAHAPAVEASTGDFTGLTWFVHDYDGTTSPSHGGNWPGTRSFLQLIPDHDFAVV
HHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEE
VLVHSDRGAEAYKKVANAMVKAFTKIESNSPVSVGATPSVLDPFVGVFKGNSQNIEIRKD
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCCCEEEEEEC
GDKYVFVQFSALTKGADPAPANVENTAEGYFIITEGPNKGALGELLKDPSGELNYVRFNH
CCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCEEEEEEEE
RIFIRGEGAVNEFDLSGSVFDK
EEEEEECCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8440253 [H]