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The map label for this gene is nrfB [H]

Identifier: 27366303

GI number: 27366303

Start: 3099620

End: 3100177

Strand: Reverse

Name: nrfB [H]

Synonym: VV1_3034

Alternate gene names: 27366303

Gene position: 3100177-3099620 (Counterclockwise)

Preceding gene: 27366305

Following gene: 326424121

Centisome position: 94.46

GC content: 46.24

Gene sequence:

>558_bases
ATGCTGAAATCTATTCTCGCATTCTCTTTCTATGGCTATTCATTCTCAGCGATAGCAGACACTTCTGTAGCCACAGAAGC
CACTGACTCGTCGCGATTCCAGGTCGAACTGATCCGTGACCGTGATTACAAATGCGTTCAATGTCATAAGGACTCGAAGG
AAACGTTGTCCGGTTCTCATGGTGAAGATGCGCATAAAATTCTCGGTCGCGAAGTCAATTGTACGGAATGTCACAACAAC
ATTGGACCAGACCATCGGGAAGGCGCACCACTTGTTACGAAATACTCTCCAGCGCAATCGAAAGAAGGGACGGAAAAAGT
GTTCATGGACACCAGTGCTATCCTGAAAGCCAATGGTGCTTGTACCGATTGTCATCAACCTCAGGATCTCCAACAAGCCA
GTTGGACCCATGATGTGCATGCGAAAAATCTAACCTGCTCGAATTGTCATGATGTTCATGCCAATAAAGCCAAAGTGTTG
GGCTTGGAGCGCAAAGAGAAAATCAAGATGTGTGTGGATTGCCACTCTGACTTTAACCAGAAAGAAGAGGAGAGGTAA

Upstream 100 bases:

>100_bases
GAATTAATTTATCACCAAAGAAATAATTGAAAATGATTCTTAATTATTATTTGGATAAGGAAAGGATAAAATGGGCAATA
TAAAATTGGCCATAGTCATA

Downstream 100 bases:

>100_bases
TATGAGTTGTTCAAGACGAAATTTCCTTGCTGGTGGTGGCGCGTTGATCATGACAACGGGCATTGCTGGCACTTCTATGG
TGACTGGGCGCTTAGCATTT

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MLKSILAFSFYGYSFSAIADTSVATEATDSSRFQVELIRDRDYKCVQCHKDSKETLSGSHGEDAHKILGREVNCTECHNN
IGPDHREGAPLVTKYSPAQSKEGTEKVFMDTSAILKANGACTDCHQPQDLQQASWTHDVHAKNLTCSNCHDVHANKAKVL
GLERKEKIKMCVDCHSDFNQKEEER

Sequences:

>Translated_185_residues
MLKSILAFSFYGYSFSAIADTSVATEATDSSRFQVELIRDRDYKCVQCHKDSKETLSGSHGEDAHKILGREVNCTECHNN
IGPDHREGAPLVTKYSPAQSKEGTEKVFMDTSAILKANGACTDCHQPQDLQQASWTHDVHAKNLTCSNCHDVHANKAKVL
GLERKEKIKMCVDCHSDFNQKEEER
>Mature_185_residues
MLKSILAFSFYGYSFSAIADTSVATEATDSSRFQVELIRDRDYKCVQCHKDSKETLSGSHGEDAHKILGREVNCTECHNN
IGPDHREGAPLVTKYSPAQSKEGTEKVFMDTSAILKANGACTDCHQPQDLQQASWTHDVHAKNLTCSNCHDVHANKAKVL
GLERKEKIKMCVDCHSDFNQKEEER

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=181, Percent_Identity=33.1491712707182, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20676; Mature: 20676

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSILAFSFYGYSFSAIADTSVATEATDSSRFQVELIRDRDYKCVQCHKDSKETLSGSH
CCHHHHHHHHCCCEEEHHCCCCHHCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHCCCC
GEDAHKILGREVNCTECHNNIGPDHREGAPLVTKYSPAQSKEGTEKVFMDTSAILKANGA
CCHHHHHHCCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCHHHHHCCCCC
CTDCHQPQDLQQASWTHDVHAKNLTCSNCHDVHANKAKVLGLERKEKIKMCVDCHSDFNQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCC
KEEER
CCCCC
>Mature Secondary Structure
MLKSILAFSFYGYSFSAIADTSVATEATDSSRFQVELIRDRDYKCVQCHKDSKETLSGSH
CCHHHHHHHHCCCEEEHHCCCCHHCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHCCCC
GEDAHKILGREVNCTECHNNIGPDHREGAPLVTKYSPAQSKEGTEKVFMDTSAILKANGA
CCHHHHHHCCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCHHHHHCCCCC
CTDCHQPQDLQQASWTHDVHAKNLTCSNCHDVHANKAKVLGLERKEKIKMCVDCHSDFNQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCC
KEEER
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]