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The map label for this gene is pyrF [H]

Identifier: 27366253

GI number: 27366253

Start: 3044464

End: 3045162

Strand: Reverse

Name: pyrF [H]

Synonym: VV1_2977

Alternate gene names: 27366253

Gene position: 3045162-3044464 (Counterclockwise)

Preceding gene: 27366254

Following gene: 326424109

Centisome position: 92.79

GC content: 46.07

Gene sequence:

>699_bases
ATGATCGACCAAAAAGTTATTGTCGCACTGGATTACGATAATCAAGCCGATGCACTGGCCTTCGTAGATAGAATTGATCC
TGCGTCATGTCGCCTAAAAGTAGGCAAGGAAATGTTTACTCTATTTGGTCCTGATTTCGTACGTGAACTTCACAAACGTG
GTTTTTCTGTATTTCTAGATCTGAAGTTTCACGATATCCCAAATACATGCTCAAAAGCCGTTCGAGCAGCAGCTGAGTTG
GGTGTCTGGATGGTGAATGTACATGCAAGTGGTGGTGAACGCATGATGACAGCATCTCGTGAGATTCTTGAACCTTATGG
TAAAGATCGCCCGTTACTGATTGGTGTGACCGTACTCACCAGCATGGAGCAAAGCGACCTAGCAGGCATTGGTTTGGACG
TTGCACCACAAGAACACGTGATTCGCTTAGCTACGTTGACGAAGAATTCTGGTTTGGATGGTGTTGTGTGTTCTGCTCAA
GAATCCTCTCTGCTGAAGAATGAGCTGGGCAAAGAGTTTAAATTGGTTACGCCAGGTATTCGCCCGCTCGGCTCAGAACA
AGGAGATCAACGTCGTATCATGACACCACTTGAAGCGGTACAAGCAGGTTCTGATTACCTTGTTATCGGACGGCCAATTA
CGCAGGCAGTAGATCCTGCGGCAGTCTTGCAAGCTATAAATACAAGCCTAACCAAATAG

Upstream 100 bases:

>100_bases
ATTGGATGGCGAATAGTTTCAATGCAGCTGACAGAGTTTCTGTCAGCTGCATTTTTTTAACATTTTATTTAATAAGAACC
AAAAACAGTTAGGAGATGAA

Downstream 100 bases:

>100_bases
CTTGAGCTCATAGATTGATACTTATGCTGTTTTTATTGTGATACTTGTCGCAAAATAAATGGCGCGTTATGAAAATATTT
ATATTGAATTTTTATGCACT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIDQKVIVALDYDNQADALAFVDRIDPASCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEL
GVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLTSMEQSDLAGIGLDVAPQEHVIRLATLTKNSGLDGVVCSAQ
ESSLLKNELGKEFKLVTPGIRPLGSEQGDQRRIMTPLEAVQAGSDYLVIGRPITQAVDPAAVLQAINTSLTK

Sequences:

>Translated_232_residues
MIDQKVIVALDYDNQADALAFVDRIDPASCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEL
GVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLTSMEQSDLAGIGLDVAPQEHVIRLATLTKNSGLDGVVCSAQ
ESSLLKNELGKEFKLVTPGIRPLGSEQGDQRRIMTPLEAVQAGSDYLVIGRPITQAVDPAAVLQAINTSLTK
>Mature_232_residues
MIDQKVIVALDYDNQADALAFVDRIDPASCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEL
GVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLTSMEQSDLAGIGLDVAPQEHVIRLATLTKNSGLDGVVCSAQ
ESSLLKNELGKEFKLVTPGIRPLGSEQGDQRRIMTPLEAVQAGSDYLVIGRPITQAVDPAAVLQAINTSLTK

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=227, Percent_Identity=71.3656387665198, Blast_Score=332, Evalue=1e-92,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25217; Mature: 25217

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQKVIVALDYDNQADALAFVDRIDPASCRLKVGKEMFTLFGPDFVRELHKRGFSVFLD
CCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVRAAAELGVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHH
SMEQSDLAGIGLDVAPQEHVIRLATLTKNSGLDGVVCSAQESSLLKNELGKEFKLVTPGI
CCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCEEECCHHHHHHHHHCCCEEEEECCCC
RPLGSEQGDQRRIMTPLEAVQAGSDYLVIGRPITQAVDPAAVLQAINTSLTK
CCCCCCCCCCCHHHCHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDQKVIVALDYDNQADALAFVDRIDPASCRLKVGKEMFTLFGPDFVRELHKRGFSVFLD
CCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVRAAAELGVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHH
SMEQSDLAGIGLDVAPQEHVIRLATLTKNSGLDGVVCSAQESSLLKNELGKEFKLVTPGI
CCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCEEECCHHHHHHHHHCCCEEEEECCCC
RPLGSEQGDQRRIMTPLEAVQAGSDYLVIGRPITQAVDPAAVLQAINTSLTK
CCCCCCCCCCCHHHCHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA