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The map label for this gene is bioF

Identifier: 27366218

GI number: 27366218

Start: 3004840

End: 3005997

Strand: Reverse

Name: bioF

Synonym: VV1_2942

Alternate gene names: 27366218

Gene position: 3005997-3004840 (Counterclockwise)

Preceding gene: 326424102

Following gene: 27366217

Centisome position: 91.59

GC content: 49.57

Gene sequence:

>1158_bases
ATGACGCAAGCCTTTAACGAAAGAATCGCGCAAGCCCTGAAGCAGAGACGTGAACAGGGCTTGAGTCGCCAAAGTGAAGT
GATTTTCTCGGGAAATCAAACGGTTTTAGAACATCAAGGGAAGCGCTATCTCAATTTTTCTGCCAACGACTATTTGGGGT
TGGCGAACGATCAATCTTTAGTTCGAGCTTGGCAGCAGGGTTTGAGTTTATATGGTTGTGGCAGCGGGGCATCTCCATTG
GTGACGGGCTACACGCCTGCGCACAGTAATCTCGCGGCTTCCTTATGCGATTGGCTAGGGTATGAAAGTGCCACTTTGTT
TGGATCGGGATTTTCGGCCAATCAAGCGTTGTTGTTTGCACTGCTTGAAAAAGGCGATCTCCTAGTGCAAGACAAATTAA
ATCACGCTTCTTTAATCGAGGCGGGGCTTTTGTCTCCTGCTACCATGAAGCGATTTAAGCATAATGATCTTAAAGCACTA
GATACGATTTTAAACCGATCTGATTGCCCAAGTTTGGTGGTGACGGAAGGCGTATTTAGCATGGATGGTGATTGTTCACC
ATTGGCTGAAATGCACGCGCTCACCCAACGCCATTCGGCGAGCTTAATGGTGGACGATGCGCATGGTGTTGGCGTGCTCG
GTGAAGAAGGTCGCGGAAGCTGTGCGCTCGCTAGTGTGAAGCCAGATTTTTTGGTGGTCACCTTTGGTAAAGCTTTCGGC
TTATCGGGGGCTGCACTTCTAACGGATAAATCCAGCGGTGATTTCCTTGCTCAATTTGCTCGTCACCACGTTTACTCTAC
CGCGCTTCCTCCTGCACAAGCTTTCGCGTTGACGCATGCAGTGGAGATGATTCGAACGCAGCAGTGGCGACGAGACAAGC
TTAATGAGCTACAAACCCTTTTTGCGGAATATTTGGGGGAGCACGATAGTTTTGTGGCCACTCAAACGCCGATCAAACCG
TGGCTTATTGGAGAGACACAACAAGCCGTGATGGTTGCACAGCGTTGCCGAGAGCAAGGCATTTGGTTGACAGCGATTCG
TCCGCCGACGGTACCCCAAAACACGGCACGCCTAAGAATCACGTTGTCTGCTAATCACACAAAAGAACAGATGCACACAC
TGGCACAAGTACTGCTGACAGTTACAGGAGAGCACTAA

Upstream 100 bases:

>100_bases
CAGCAAGTCTCACAAAAACCTGATGAAATTACAGAAAACGAATTGTTAGATCGCGTAGTCGAAAGCGTGGCTGCTCGCCC
AACAAAAGACGATCTGTTTT

Downstream 100 bases:

>100_bases
TGGAAATGGCTAAGGTATCCTTTACTGCGGAGGAAGCGTATGTGGATAAGCTGGCTATTGCTCAAGCATTCGGTAAGGCG
GCTAAAAGCTATGACCAGCA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTQAFNERIAQALKQRREQGLSRQSEVIFSGNQTVLEHQGKRYLNFSANDYLGLANDQSLVRAWQQGLSLYGCGSGASPL
VTGYTPAHSNLAASLCDWLGYESATLFGSGFSANQALLFALLEKGDLLVQDKLNHASLIEAGLLSPATMKRFKHNDLKAL
DTILNRSDCPSLVVTEGVFSMDGDCSPLAEMHALTQRHSASLMVDDAHGVGVLGEEGRGSCALASVKPDFLVVTFGKAFG
LSGAALLTDKSSGDFLAQFARHHVYSTALPPAQAFALTHAVEMIRTQQWRRDKLNELQTLFAEYLGEHDSFVATQTPIKP
WLIGETQQAVMVAQRCREQGIWLTAIRPPTVPQNTARLRITLSANHTKEQMHTLAQVLLTVTGEH

Sequences:

>Translated_385_residues
MTQAFNERIAQALKQRREQGLSRQSEVIFSGNQTVLEHQGKRYLNFSANDYLGLANDQSLVRAWQQGLSLYGCGSGASPL
VTGYTPAHSNLAASLCDWLGYESATLFGSGFSANQALLFALLEKGDLLVQDKLNHASLIEAGLLSPATMKRFKHNDLKAL
DTILNRSDCPSLVVTEGVFSMDGDCSPLAEMHALTQRHSASLMVDDAHGVGVLGEEGRGSCALASVKPDFLVVTFGKAFG
LSGAALLTDKSSGDFLAQFARHHVYSTALPPAQAFALTHAVEMIRTQQWRRDKLNELQTLFAEYLGEHDSFVATQTPIKP
WLIGETQQAVMVAQRCREQGIWLTAIRPPTVPQNTARLRITLSANHTKEQMHTLAQVLLTVTGEH
>Mature_384_residues
TQAFNERIAQALKQRREQGLSRQSEVIFSGNQTVLEHQGKRYLNFSANDYLGLANDQSLVRAWQQGLSLYGCGSGASPLV
TGYTPAHSNLAASLCDWLGYESATLFGSGFSANQALLFALLEKGDLLVQDKLNHASLIEAGLLSPATMKRFKHNDLKALD
TILNRSDCPSLVVTEGVFSMDGDCSPLAEMHALTQRHSASLMVDDAHGVGVLGEEGRGSCALASVKPDFLVVTFGKAFGL
SGAALLTDKSSGDFLAQFARHHVYSTALPPAQAFALTHAVEMIRTQQWRRDKLNELQTLFAEYLGEHDSFVATQTPIKPW
LIGETQQAVMVAQRCREQGIWLTAIRPPTVPQNTARLRITLSANHTKEQMHTLAQVLLTVTGEH

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=348, Percent_Identity=30.7471264367816, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI284448556, Length=348, Percent_Identity=30.7471264367816, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI119220554, Length=371, Percent_Identity=30.188679245283, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=29.8630136986301, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI83977442, Length=350, Percent_Identity=29.7142857142857, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI83977444, Length=350, Percent_Identity=29.7142857142857, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI83977440, Length=350, Percent_Identity=29.7142857142857, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI4502025, Length=346, Percent_Identity=30.0578034682081, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI40316939, Length=346, Percent_Identity=30.0578034682081, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI5454084, Length=192, Percent_Identity=26.0416666666667, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1786993, Length=345, Percent_Identity=51.5942028985507, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI1790046, Length=380, Percent_Identity=33.1578947368421, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71994529, Length=367, Percent_Identity=29.9727520435967, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17560914, Length=382, Percent_Identity=28.7958115183246, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560912, Length=382, Percent_Identity=28.7958115183246, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566772, Length=373, Percent_Identity=25.4691689008043, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71982617, Length=275, Percent_Identity=24.7272727272727, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71982625, Length=275, Percent_Identity=24.7272727272727, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320438, Length=373, Percent_Identity=29.2225201072386, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320267, Length=360, Percent_Identity=27.2222222222222, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323954, Length=303, Percent_Identity=25.7425742574257, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24662918, Length=372, Percent_Identity=31.1827956989247, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17137420, Length=394, Percent_Identity=29.6954314720812, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17136286, Length=381, Percent_Identity=30.1837270341207, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653280, Length=252, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24653276, Length=252, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24653278, Length=252, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_VIBVU (Q8D8N0)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761746.1
- HSSP:   P12998
- ProteinModelPortal:   Q8D8N0
- GeneID:   1179776
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2942
- HOGENOM:   HBG649839
- OMA:   KDYASND
- ProtClustDB:   PRK05958
- BioCyc:   VVUL216895:VV1_2942-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 42033; Mature: 41902

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 23-23 BINDING 135-135 BINDING 180-180 BINDING 350-350

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAFNERIAQALKQRREQGLSRQSEVIFSGNQTVLEHQGKRYLNFSANDYLGLANDQSL
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCCEEEECCCCCCEECCCCHHH
VRAWQQGLSLYGCGSGASPLVTGYTPAHSNLAASLCDWLGYESATLFGSGFSANQALLFA
HHHHHHCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHH
LLEKGDLLVQDKLNHASLIEAGLLSPATMKRFKHNDLKALDTILNRSDCPSLVVTEGVFS
HHHCCCEEEECCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCC
MDGDCSPLAEMHALTQRHSASLMVDDAHGVGVLGEEGRGSCALASVKPDFLVVTFGKAFG
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCEEEEECCCCEEEEEECHHHC
LSGAALLTDKSSGDFLAQFARHHVYSTALPPAQAFALTHAVEMIRTQQWRRDKLNELQTL
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAEYLGEHDSFVATQTPIKPWLIGETQQAVMVAQRCREQGIWLTAIRPPTVPQNTARLRI
HHHHHCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEE
TLSANHTKEQMHTLAQVLLTVTGEH
EEECCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQAFNERIAQALKQRREQGLSRQSEVIFSGNQTVLEHQGKRYLNFSANDYLGLANDQSL
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCCEEEECCCCCCEECCCCHHH
VRAWQQGLSLYGCGSGASPLVTGYTPAHSNLAASLCDWLGYESATLFGSGFSANQALLFA
HHHHHHCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHH
LLEKGDLLVQDKLNHASLIEAGLLSPATMKRFKHNDLKALDTILNRSDCPSLVVTEGVFS
HHHCCCEEEECCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCC
MDGDCSPLAEMHALTQRHSASLMVDDAHGVGVLGEEGRGSCALASVKPDFLVVTFGKAFG
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCEEEEECCCCEEEEEECHHHC
LSGAALLTDKSSGDFLAQFARHHVYSTALPPAQAFALTHAVEMIRTQQWRRDKLNELQTL
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAEYLGEHDSFVATQTPIKPWLIGETQQAVMVAQRCREQGIWLTAIRPPTVPQNTARLRI
HHHHHCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEE
TLSANHTKEQMHTLAQVLLTVTGEH
EEECCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA