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The map label for this gene is nhaA

Identifier: 27366115

GI number: 27366115

Start: 2897647

End: 2898816

Strand: Reverse

Name: nhaA

Synonym: VV1_2833

Alternate gene names: 27366115

Gene position: 2898816-2897647 (Counterclockwise)

Preceding gene: 326424081

Following gene: 27366114

Centisome position: 88.33

GC content: 46.92

Gene sequence:

>1170_bases
ATGAACGATGTCATTCGTGACTTTTTCAAAATGGAATCTGCTGGTGGCATTTTGCTTGTGATCGCTGCCGCCATCGCAAT
GGTGATTGCAAACTCGCCTCTCAATGAAAGTTACCAAGCGGTTTTGCATACTTATGTATTTGGCATGTCAGTATCTCACT
GGATCAATGATGGCCTAATGGCAATTTTCTTCTTGCTGATCGGCTTGGAAGTTAAGCGTGAATTATTAGAAGGCGCATTG
AAATCGCGTGAAACTGCGATCTTCCCAGCGATTGCCGCAGTGGGTGGTATGTTGGCGCCAGCGCTTATCTATGTTGCTTT
CAATGGAAACGATCCGGAAGCGATTAAAGGTTGGGCAATTCCAGCAGCGACCGATATTGCATTTGCTTTGGGTATCATGG
CGCTGCTAGGTAAGCGCGTTCCTGTCAGTCTTAAAGTGTTCCTTTTGGCATTAGCGATCATCGATGACTTGGGCGTCGTT
GTGATTATTGCGCTCTTCTACAGTGGCGATTTGTCGACACTTGCGTTGACGGTTGGATTCGCGATGACGGGTGTCTTGTT
CATGCTAAACGCTAAGAATGTGACTAAGTTAATTTGGTATATCGTTGTCGGCTTTATCTTGTGGGTTGCGGTACTGAAAT
CCGGAGTCCATGCAACCTTGGCAGGTGTCGTGATTGGTTTCTCTATCCCATTACAGGGTAAAAAAGGTGAGCACTCTCCG
TTGAAACACATGGAACATGCGCTTCACCCATATGTTGCCTTTGCTATCTTACCTGTCTTTGCCTTTGCTAACGCTGGTAT
CTCGCTTGAAGGGGTGTCTCTTTCTGGCTTGACTTCCATGTTGCCGCTGGGTATCGCGCTAGGACTGTTGGTTGGTAAAC
CACTGGGTATCTTTACGTTTAGCTGGGCAGCGGTGAAGTTTGGTGTGGCTAAATTGCCTGAAGGTGTGAACTTCAAGCAT
ATCTTTGCCGTGTCAGTGCTCTGTGGTATCGGTTTCACCATGTCGATCTTTATTTCATCGCTGGCTTTCGGTGGTGCAAA
TCCAGACTTCGATACTTATTCACGACTAGGTATCTTAATGGGATCGACGACAGCGGCAGTGCTTGGTTATTTCTTACTGC
ATGTATCGCTGCCAAAGACAGCGGCGGAAAGTGAGAAAGCAATCAGCTAA

Upstream 100 bases:

>100_bases
AACATCCAAACAAGCGATTATTCCTACAAAATGGTTATATATACACTGCTTACATTCTTCAAAGTTCGTTGATTACGATT
ATAAATAAAAGGGTGAAATC

Downstream 100 bases:

>100_bases
ACGACAGACTGCTAAACCATAGATTGATGCAATAAACTCAAAAGCCTCTGCAACTATCGCAGAGGCTTTTTGGTTTCTAT
TGGCTGTCTTAACTATTTGC

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA 1

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNDVIRDFFKMESAGGILLVIAAAIAMVIANSPLNESYQAVLHTYVFGMSVSHWINDGLMAIFFLLIGLEVKRELLEGAL
KSRETAIFPAIAAVGGMLAPALIYVAFNGNDPEAIKGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVV
VIIALFYSGDLSTLALTVGFAMTGVLFMLNAKNVTKLIWYIVVGFILWVAVLKSGVHATLAGVVIGFSIPLQGKKGEHSP
LKHMEHALHPYVAFAILPVFAFANAGISLEGVSLSGLTSMLPLGIALGLLVGKPLGIFTFSWAAVKFGVAKLPEGVNFKH
IFAVSVLCGIGFTMSIFISSLAFGGANPDFDTYSRLGILMGSTTAAVLGYFLLHVSLPKTAAESEKAIS

Sequences:

>Translated_389_residues
MNDVIRDFFKMESAGGILLVIAAAIAMVIANSPLNESYQAVLHTYVFGMSVSHWINDGLMAIFFLLIGLEVKRELLEGAL
KSRETAIFPAIAAVGGMLAPALIYVAFNGNDPEAIKGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVV
VIIALFYSGDLSTLALTVGFAMTGVLFMLNAKNVTKLIWYIVVGFILWVAVLKSGVHATLAGVVIGFSIPLQGKKGEHSP
LKHMEHALHPYVAFAILPVFAFANAGISLEGVSLSGLTSMLPLGIALGLLVGKPLGIFTFSWAAVKFGVAKLPEGVNFKH
IFAVSVLCGIGFTMSIFISSLAFGGANPDFDTYSRLGILMGSTTAAVLGYFLLHVSLPKTAAESEKAIS
>Mature_389_residues
MNDVIRDFFKMESAGGILLVIAAAIAMVIANSPLNESYQAVLHTYVFGMSVSHWINDGLMAIFFLLIGLEVKRELLEGAL
KSRETAIFPAIAAVGGMLAPALIYVAFNGNDPEAIKGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVV
VIIALFYSGDLSTLALTVGFAMTGVLFMLNAKNVTKLIWYIVVGFILWVAVLKSGVHATLAGVVIGFSIPLQGKKGEHSP
LKHMEHALHPYVAFAILPVFAFANAGISLEGVSLSGLTSMLPLGIALGLLVGKPLGIFTFSWAAVKFGVAKLPEGVNFKH
IFAVSVLCGIGFTMSIFISSLAFGGANPDFDTYSRLGILMGSTTAAVLGYFLLHVSLPKTAAESEKAIS

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=382, Percent_Identity=57.3298429319372, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA1_VIBVU (Q8D8Y2)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761643.1
- HSSP:   P13738
- ProteinModelPortal:   Q8D8Y2
- SMR:   Q8D8Y2
- GeneID:   1179673
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2833
- HOGENOM:   HBG665619
- OMA:   IYLKFFA
- ProtClustDB:   PRK09560
- BioCyc:   VVUL216895:VV1_2833-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 41073; Mature: 41073

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10c4c058)-; HASH(0x10c29a1c)-; HASH(0x10a25254)-; HASH(0x106b0120)-; HASH(0x10306d1c)-; HASH(0x10c212d0)-; HASH(0x10b0bc8c)-; HASH(0x103d8bd4)-; HASH(0x10a6dc68)-; HASH(0xe634c10)-; HASH(0xf6ac8c0)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVIRDFFKMESAGGILLVIAAAIAMVIANSPLNESYQAVLHTYVFGMSVSHWINDGLM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
AIFFLLIGLEVKRELLEGALKSRETAIFPAIAAVGGMLAPALIYVAFNGNDPEAIKGWAI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCCC
PAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVVVIIALFYSGDLSTLALTVGF
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AMTGVLFMLNAKNVTKLIWYIVVGFILWVAVLKSGVHATLAGVVIGFSIPLQGKKGEHSP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCCH
LKHMEHALHPYVAFAILPVFAFANAGISLEGVSLSGLTSMLPLGIALGLLVGKPLGIFTF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
SWAAVKFGVAKLPEGVNFKHIFAVSVLCGIGFTMSIFISSLAFGGANPDFDTYSRLGILM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GSTTAAVLGYFLLHVSLPKTAAESEKAIS
CCHHHHHHHHHHHHHCCCCHHHCHHCCCH
>Mature Secondary Structure
MNDVIRDFFKMESAGGILLVIAAAIAMVIANSPLNESYQAVLHTYVFGMSVSHWINDGLM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
AIFFLLIGLEVKRELLEGALKSRETAIFPAIAAVGGMLAPALIYVAFNGNDPEAIKGWAI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCCC
PAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVVVIIALFYSGDLSTLALTVGF
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AMTGVLFMLNAKNVTKLIWYIVVGFILWVAVLKSGVHATLAGVVIGFSIPLQGKKGEHSP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCCH
LKHMEHALHPYVAFAILPVFAFANAGISLEGVSLSGLTSMLPLGIALGLLVGKPLGIFTF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
SWAAVKFGVAKLPEGVNFKHIFAVSVLCGIGFTMSIFISSLAFGGANPDFDTYSRLGILM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GSTTAAVLGYFLLHVSLPKTAAESEKAIS
CCHHHHHHHHHHHHHCCCCHHHCHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA