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The map label for this gene is ywbI [H]

Identifier: 27366064

GI number: 27366064

Start: 2840116

End: 2840985

Strand: Reverse

Name: ywbI [H]

Synonym: VV1_2777

Alternate gene names: 27366064

Gene position: 2840985-2840116 (Counterclockwise)

Preceding gene: 326424073

Following gene: 326424072

Centisome position: 86.57

GC content: 47.01

Gene sequence:

>870_bases
ATGGAATCGAAACAACTCAAACAATTTTTGGCGGTGGCCGAAGAGAAAAATTTCACTCGTGCAGCTCAGTCTTTGCATAT
CGCGCAGCCTGCCTTGAGTGTCTCAATCAAAAAGCTCGAACAAAGCCTCGGTGTGACGCTATTTAAGCGTGATGACAAAC
AGATCGCACTGACAACGGAAGGGCAAACCTTGTATGAGCATGCCAAACGTGTTGTGCAGCAACTGCATGATGCCGAGTTG
GCGATGAATGAACTGAAAGGGCTTGAGAAAGGTGAGGTGCGCCTTGGTGCGCCAAGCATGATGGGCAGCTACTTCTTTCC
AGAAATTTTAATGGCGTTTAAAAGTCACTATCCCAATCTAAAATTGACACTGATTGAAGCGGGGACGCAGTCGATTCGTA
AGATGCTGCTTGAAGGGGATTTGGACATTGGCGTGATCACGGAAAACGACGTACCGGACGATTTAGAAATAGACCACCTG
TTCTCTAGCCAGATGATGGCGGTAGTTGGCAGTGAGCATTCGTTATCGCAACGAGCATCCCTCACTTTTGAAGAGTTTTT
TCAACATGAACTGGTGATGTTCAAATCGGGTTATTTCCACCGCGATTTTCTCGATCACTTTAGCCAGCAGCATGGCATTG
AAATGCAGTATTCTTTTGAAACCAATCTATTGCCATTGATTTTGAAGATCGTAAAGCGAGAGTTTGCCATTACTGCGCTG
CTTCAATTGGTGACCGAGAATGAGAGTGGCGTGGTCGGCGTGCCATTTTCTCCGCCCGTCACTCTGAACCTAGCATTGGC
TTGGCGACGTGGCGGCTATCTGTCGGTGGCGGATCGCACCTTTATCGAATTTATAAAGCAGTATGTTTAG

Upstream 100 bases:

>100_bases
GTACATCTTAATTCTCCTACTGATTTGTGAGCACAGTATCAGTCACGTCGTGATAAACAAAATATATTAAAAATATGATG
ACGATATATTTTTGATCTAG

Downstream 100 bases:

>100_bases
CTGTAATCACTTATCGCCATACATGCGTTCAAAGTTCTTTGGGTTCCAACCAATCATCAAACGGTCACCGATTTTAAGTA
CAGGCACAGAGCGCGCACCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MESKQLKQFLAVAEEKNFTRAAQSLHIAQPALSVSIKKLEQSLGVTLFKRDDKQIALTTEGQTLYEHAKRVVQQLHDAEL
AMNELKGLEKGEVRLGAPSMMGSYFFPEILMAFKSHYPNLKLTLIEAGTQSIRKMLLEGDLDIGVITENDVPDDLEIDHL
FSSQMMAVVGSEHSLSQRASLTFEEFFQHELVMFKSGYFHRDFLDHFSQQHGIEMQYSFETNLLPLILKIVKREFAITAL
LQLVTENESGVVGVPFSPPVTLNLALAWRRGGYLSVADRTFIEFIKQYV

Sequences:

>Translated_289_residues
MESKQLKQFLAVAEEKNFTRAAQSLHIAQPALSVSIKKLEQSLGVTLFKRDDKQIALTTEGQTLYEHAKRVVQQLHDAEL
AMNELKGLEKGEVRLGAPSMMGSYFFPEILMAFKSHYPNLKLTLIEAGTQSIRKMLLEGDLDIGVITENDVPDDLEIDHL
FSSQMMAVVGSEHSLSQRASLTFEEFFQHELVMFKSGYFHRDFLDHFSQQHGIEMQYSFETNLLPLILKIVKREFAITAL
LQLVTENESGVVGVPFSPPVTLNLALAWRRGGYLSVADRTFIEFIKQYV
>Mature_289_residues
MESKQLKQFLAVAEEKNFTRAAQSLHIAQPALSVSIKKLEQSLGVTLFKRDDKQIALTTEGQTLYEHAKRVVQQLHDAEL
AMNELKGLEKGEVRLGAPSMMGSYFFPEILMAFKSHYPNLKLTLIEAGTQSIRKMLLEGDLDIGVITENDVPDDLEIDHL
FSSQMMAVVGSEHSLSQRASLTFEEFFQHELVMFKSGYFHRDFLDHFSQQHGIEMQYSFETNLLPLILKIVKREFAITAL
LQLVTENESGVVGVPFSPPVTLNLALAWRRGGYLSVADRTFIEFIKQYV

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=30.0353356890459, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI145693105, Length=287, Percent_Identity=24.7386759581882, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787879, Length=198, Percent_Identity=31.3131313131313, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=26.5993265993266, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=26.027397260274, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788748, Length=285, Percent_Identity=24.2105263157895, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI2367136, Length=268, Percent_Identity=27.6119402985075, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=25.8620689655172, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081904, Length=214, Percent_Identity=27.5700934579439, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787601, Length=152, Percent_Identity=29.6052631578947, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=23.9202657807309, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787530, Length=222, Percent_Identity=24.3243243243243, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789204, Length=265, Percent_Identity=23.3962264150943, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788297, Length=151, Percent_Identity=31.7880794701987, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082132, Length=295, Percent_Identity=26.4406779661017, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=26.2096774193548, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788296, Length=216, Percent_Identity=26.3888888888889, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789506, Length=296, Percent_Identity=23.6486486486486, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789173, Length=261, Percent_Identity=26.0536398467433, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=124, Percent_Identity=29.8387096774194, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=26.2068965517241, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790783, Length=178, Percent_Identity=29.7752808988764, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=185, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI48994962, Length=172, Percent_Identity=30.8139534883721, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=27.0491803278689, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32771; Mature: 32771

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKQLKQFLAVAEEKNFTRAAQSLHIAQPALSVSIKKLEQSLGVTLFKRDDKQIALTTE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCEEEEECCCCEEEEEEC
GQTLYEHAKRVVQQLHDAELAMNELKGLEKGEVRLGAPSMMGSYFFPEILMAFKSHYPNL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCE
KLTLIEAGTQSIRKMLLEGDLDIGVITENDVPDDLEIDHLFSSQMMAVVGSEHSLSQRAS
EEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
LTFEEFFQHELVMFKSGYFHRDFLDHFSQQHGIEMQYSFETNLLPLILKIVKREFAITAL
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LQLVTENESGVVGVPFSPPVTLNLALAWRRGGYLSVADRTFIEFIKQYV
HHHHHCCCCCEEECCCCCCEEEEEEEEEECCCEEEHHHHHHHHHHHHHC
>Mature Secondary Structure
MESKQLKQFLAVAEEKNFTRAAQSLHIAQPALSVSIKKLEQSLGVTLFKRDDKQIALTTE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCEEEEECCCCEEEEEEC
GQTLYEHAKRVVQQLHDAELAMNELKGLEKGEVRLGAPSMMGSYFFPEILMAFKSHYPNL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCE
KLTLIEAGTQSIRKMLLEGDLDIGVITENDVPDDLEIDHLFSSQMMAVVGSEHSLSQRAS
EEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
LTFEEFFQHELVMFKSGYFHRDFLDHFSQQHGIEMQYSFETNLLPLILKIVKREFAITAL
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LQLVTENESGVVGVPFSPPVTLNLALAWRRGGYLSVADRTFIEFIKQYV
HHHHHCCCCCEEECCCCCCEEEEEEEEEECCCEEEHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]