Click here to switch to the map view.

The map label for this gene is ttcA [H]

Identifier: 27365903

GI number: 27365903

Start: 2647470

End: 2648402

Strand: Reverse

Name: ttcA [H]

Synonym: VV1_2608

Alternate gene names: 27365903

Gene position: 2648402-2647470 (Counterclockwise)

Preceding gene: 161501806

Following gene: 27365899

Centisome position: 80.7

GC content: 42.77

Gene sequence:

>933_bases
ATGACAGAGCAAATTCAAGAGCGCACTAAAGCTCAACAATATAACTTCAACAAGTTACAAAAGCGTATTCGTCGTAATAC
AGGCCAAGCGATTGCTGACTTCAACATGATCGAAGACGGTGACCGTATTATGGTGTGTTTGTCTGGTGGTAAAGACAGCT
TTACCATGTTGGACATCCTGATTAGCTTACAAAAAAGTGCCCCAATCTCCTTTTCTCTTGTCGCTGTTAACCTAGACCAA
AAGCAGCCGGGCTTTCCAGCGCATGTACTGCCTGAATACCTAGAAAGCTTAGGTGTGGAATATAAGATCGTAGAAGAAGA
TACTTACTCGATTGTTCAAGATAAGATCCCTGAAGGTAAAACGACCTGTTCACTTTGCTCTCGACTACGTCGTGGTATTT
TGTACCGCACAGCCAAAGAACTCGGTGCAACAAAAATTGCGCTAGGCCATCATCGCGACGACATTTTGGAAACCTTATTT
CTAAACATGTTCTACGGTGGAAAAATGAAAGGCATGCCACCTAAGTTGGTTTCAGATAATGGCGAACACGTTGTGATTCG
TCCACTGGCATACTGCCGTGAGAAAGACATCATCAAATATTCAGATATGGCTGGCTACCCTATCATTCCATGTAACCTAT
GTGGCTCTCAGCCAAACTTGCAGCGTCAAAATATCAAACAGATGCTGAATGACTGGGATAAGCGTTTCCCAGGTCGTATT
GAGACAATGTTCCGTGCAATGCAGAATGTGGTGCCAAGCCATTTAGCGGATTTTGAGTTGTTTGATTTTAAATCGATCAA
CAAAGATTCAGGCGTGATTAACGGTGGTGATATCGGTTTCGACAAAGAAGAAATGCCTGTTGCAACCGTCGAGGATGAAG
ATATGGTGCAAGAGTTTGATCCAAGCTTAAAGCTAGATGTGACCAATATTTAA

Upstream 100 bases:

>100_bases
TAATTAGCCCGGGATTTTTTTGTCCGGTACTGGCATCTTGGAAATTTATCCGTATCATACGCACTTCATTTATTTTGATG
TGTTTTAAGACTAACAGCCG

Downstream 100 bases:

>100_bases
TCAGCTTACAAACACAATAAAGGCTACCATGTTGGTAGCCTTTATTTTATCACTTGAGTGTTCAACTCATTATTTCTTTG
CCGCAGGTAAGTAAGGCAAA

Product: tRNA 2-thiocytidine biosynthesis protein TtcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTEQIQERTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILISLQKSAPISFSLVAVNLDQ
KQPGFPAHVLPEYLESLGVEYKIVEEDTYSIVQDKIPEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETLF
LNMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMAGYPIIPCNLCGSQPNLQRQNIKQMLNDWDKRFPGRI
ETMFRAMQNVVPSHLADFELFDFKSINKDSGVINGGDIGFDKEEMPVATVEDEDMVQEFDPSLKLDVTNI

Sequences:

>Translated_310_residues
MTEQIQERTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILISLQKSAPISFSLVAVNLDQ
KQPGFPAHVLPEYLESLGVEYKIVEEDTYSIVQDKIPEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETLF
LNMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMAGYPIIPCNLCGSQPNLQRQNIKQMLNDWDKRFPGRI
ETMFRAMQNVVPSHLADFELFDFKSINKDSGVINGGDIGFDKEEMPVATVEDEDMVQEFDPSLKLDVTNI
>Mature_309_residues
TEQIQERTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILISLQKSAPISFSLVAVNLDQK
QPGFPAHVLPEYLESLGVEYKIVEEDTYSIVQDKIPEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETLFL
NMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMAGYPIIPCNLCGSQPNLQRQNIKQMLNDWDKRFPGRIE
TMFRAMQNVVPSHLADFELFDFKSINKDSGVINGGDIGFDKEEMPVATVEDEDMVQEFDPSLKLDVTNI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=310, Percent_Identity=75.4838709677419, Blast_Score=493, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI193211090, Length=249, Percent_Identity=30.5220883534137, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17540090, Length=232, Percent_Identity=27.1551724137931, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24651972, Length=222, Percent_Identity=27.9279279279279, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35183; Mature: 35052

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQIQERTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDIL
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHCCEECCCCEEEEEEECCCCHHHHHHHH
ISLQKSAPISFSLVAVNLDQKQPGFPAHVLPEYLESLGVEYKIVEEDTYSIVQDKIPEGK
HHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCC
TTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GEHVVIRPLAYCREKDIIKYSDMAGYPIIPCNLCGSQPNLQRQNIKQMLNDWDKRFPGRI
CCEEEECCHHHHCCCCCEEECCCCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHH
ETMFRAMQNVVPSHLADFELFDFKSINKDSGVINGGDIGFDKEEMPVATVEDEDMVQEFD
HHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEECCCCCCCCCCCCCEEECCCHHHHHHCC
PSLKLDVTNI
CCCEEEECCC
>Mature Secondary Structure 
TEQIQERTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDIL
CHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHCCEECCCCEEEEEEECCCCHHHHHHHH
ISLQKSAPISFSLVAVNLDQKQPGFPAHVLPEYLESLGVEYKIVEEDTYSIVQDKIPEGK
HHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCC
TTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GEHVVIRPLAYCREKDIIKYSDMAGYPIIPCNLCGSQPNLQRQNIKQMLNDWDKRFPGRI
CCEEEECCHHHHCCCCCEEECCCCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHH
ETMFRAMQNVVPSHLADFELFDFKSINKDSGVINGGDIGFDKEEMPVATVEDEDMVQEFD
HHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEECCCCCCCCCCCCCEEECCCHHHHHHCC
PSLKLDVTNI
CCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA