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The map label for this gene is insI1 [C]

Identifier: 27365847

GI number: 27365847

Start: 2587127

End: 2588095

Strand: Reverse

Name: insI1 [C]

Synonym: VV1_2548

Alternate gene names: 27365847

Gene position: 2588095-2587127 (Counterclockwise)

Preceding gene: 27365852

Following gene: 27365840

Centisome position: 78.86

GC content: 45.3

Gene sequence:

>969_bases
ATGAGATACAAGCATCTCACTGAGAACGAAAGGTATATGATTTCTGCGCTCAGAAAGCAAGGGATTAGTACCGCTAAAAT
AGCTAAACAACTGGGGCGTCACAAAGCCACTATCTATCGAGAAATTGAACGCAACTCCCGATACAACAGACACTTTAAAA
GGTACTCCTATCAGGCATGGAGAGCCCAACAAATGGCTCGCAACCGGCTAAGCCGCTCGCGCAGAAACAAGCGCTACAGC
GAAATCGACTTCAGGTTGCCTGAGGCCTTGCTACGACTGGATTGGAGCCCTGACCAAATCGTCGGATACCTAAGGGTAAG
AGGCTATCCAACAATGAGCCACGAGCTCATTTATCAGCATATTTGGAACGACAAAACTCTCGGTGGAACACTATGGAAAC
ACCTACGCCAATCGACCAAGAAAAGGCGTAAAAGGTATAACTCAAAAGACAGCCGAGGTCGGCTGGCAGCAAAGCGTCAT
ATTACCGAAAGACCTGCAAAAGCTGAGCATCGAAAAGAGCCTGGTCATTGGGAAATTGATACCGTCGTTGGACGCGGAAC
CAAGCACTGTATCGTGACTTTAGTCGACAGAATGACTGGCTACACTTTTATTGGGCAAATGGACGATAGAACAAGCGAGT
CGCTCAACGTAAGAATGAGCAAAATCATGACCCGCTCTGACTTACCTTTTAAGACGATAACTGCGGATAACGGCACTGAA
TTTCATGGTTATGCACAACTGGAAAAACATCATAACTGTCTGTTTTACTTTGCAAATCCATACCATTCATGGGAGCGAGG
GACTAATGAAAACACCAATGGTTTGATACGACAATACTTACCAAAACGAACTTCTATGTCACACGTAACACAGAAGCTCT
GCAATGAAATTGCACACAAATTAAATACGCGCCCACGCAAAAGACTTGGGTATAGGACACCAACGGAGTATATTCATGCG
CATCTGTAA

Upstream 100 bases:

>100_bases
TTATGCAACTTGGATTCAAGTACGAAGTGCGACACCTCTGAACATAAAAACAGTGAGATGCGATAATCATGAAGTTTGCT
CCCGCCAAGAAGTAAAAAAC

Downstream 100 bases:

>100_bases
AAAGTGTCGCACTTCAAACTTGATACTAAGACACAATTGCTACACATCACTTTCCGTTAGCATTTGACGACAATTTCTTA
CTGTCAGGACACGAATTTCA

Product: transposase and inactivated derivatives

Products: NA

Alternate protein names: Transposon TN4551 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRYKHLTENERYMISALRKQGISTAKIAKQLGRHKATIYREIERNSRYNRHFKRYSYQAWRAQQMARNRLSRSRRNKRYS
EIDFRLPEALLRLDWSPDQIVGYLRVRGYPTMSHELIYQHIWNDKTLGGTLWKHLRQSTKKRRKRYNSKDSRGRLAAKRH
ITERPAKAEHRKEPGHWEIDTVVGRGTKHCIVTLVDRMTGYTFIGQMDDRTSESLNVRMSKIMTRSDLPFKTITADNGTE
FHGYAQLEKHHNCLFYFANPYHSWERGTNENTNGLIRQYLPKRTSMSHVTQKLCNEIAHKLNTRPRKRLGYRTPTEYIHA
HL

Sequences:

>Translated_322_residues
MRYKHLTENERYMISALRKQGISTAKIAKQLGRHKATIYREIERNSRYNRHFKRYSYQAWRAQQMARNRLSRSRRNKRYS
EIDFRLPEALLRLDWSPDQIVGYLRVRGYPTMSHELIYQHIWNDKTLGGTLWKHLRQSTKKRRKRYNSKDSRGRLAAKRH
ITERPAKAEHRKEPGHWEIDTVVGRGTKHCIVTLVDRMTGYTFIGQMDDRTSESLNVRMSKIMTRSDLPFKTITADNGTE
FHGYAQLEKHHNCLFYFANPYHSWERGTNENTNGLIRQYLPKRTSMSHVTQKLCNEIAHKLNTRPRKRLGYRTPTEYIHA
HL
>Mature_322_residues
MRYKHLTENERYMISALRKQGISTAKIAKQLGRHKATIYREIERNSRYNRHFKRYSYQAWRAQQMARNRLSRSRRNKRYS
EIDFRLPEALLRLDWSPDQIVGYLRVRGYPTMSHELIYQHIWNDKTLGGTLWKHLRQSTKKRRKRYNSKDSRGRLAAKRH
ITERPAKAEHRKEPGHWEIDTVVGRGTKHCIVTLVDRMTGYTFIGQMDDRTSESLNVRMSKIMTRSDLPFKTITADNGTE
FHGYAQLEKHHNCLFYFANPYHSWERGTNENTNGLIRQYLPKRTSMSHVTQKLCNEIAHKLNTRPRKRLGYRTPTEYIHA
HL

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=330, Percent_Identity=36.0606060606061, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1790736, Length=330, Percent_Identity=36.0606060606061, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787671, Length=330, Percent_Identity=36.0606060606061, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 38486; Mature: 38486

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKHLTENERYMISALRKQGISTAKIAKQLGRHKATIYREIERNSRYNRHFKRYSYQAW
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RAQQMARNRLSRSRRNKRYSEIDFRLPEALLRLDWSPDQIVGYLRVRGYPTMSHELIYQH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCHHHHHHHHH
IWNDKTLGGTLWKHLRQSTKKRRKRYNSKDSRGRLAAKRHITERPAKAEHRKEPGHWEID
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCEEEH
TVVGRGTKHCIVTLVDRMTGYTFIGQMDDRTSESLNVRMSKIMTRSDLPFKTITADNGTE
HHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
FHGYAQLEKHHNCLFYFANPYHSWERGTNENTNGLIRQYLPKRTSMSHVTQKLCNEIAHK
CCHHHHHHCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LNTRPRKRLGYRTPTEYIHAHL
HCCCHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MRYKHLTENERYMISALRKQGISTAKIAKQLGRHKATIYREIERNSRYNRHFKRYSYQAW
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RAQQMARNRLSRSRRNKRYSEIDFRLPEALLRLDWSPDQIVGYLRVRGYPTMSHELIYQH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCHHHHHHHHH
IWNDKTLGGTLWKHLRQSTKKRRKRYNSKDSRGRLAAKRHITERPAKAEHRKEPGHWEID
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCEEEH
TVVGRGTKHCIVTLVDRMTGYTFIGQMDDRTSESLNVRMSKIMTRSDLPFKTITADNGTE
HHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
FHGYAQLEKHHNCLFYFANPYHSWERGTNENTNGLIRQYLPKRTSMSHVTQKLCNEIAHK
CCHHHHHHCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LNTRPRKRLGYRTPTEYIHAHL
HCCCHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2820936 [H]