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The map label for this gene is emrD [H]

Identifier: 27365670

GI number: 27365670

Start: 2380093

End: 2381340

Strand: Reverse

Name: emrD [H]

Synonym: VV1_2351

Alternate gene names: 27365670

Gene position: 2381340-2380093 (Counterclockwise)

Preceding gene: 27365671

Following gene: 27365665

Centisome position: 72.56

GC content: 50.96

Gene sequence:

>1248_bases
ATGGTCTTTTTCGTTTTTAATTTAGCCGGAGAATTTGCGCAGATGTCCGCGTCATTTCCATTAGCCAAGCTTACCCTTTT
GATCGCTATTTTGACCGCTGTGGGTCAAATGACTCAAACCATGTACGTGCCGTCAATCGGTCACATGGCCAGTGAGTTTT
TGGTATCGCCTGCTGCACTGCAGGCCGTCATGGCGTGCTATTTGATCCCTTACGGATTATCGCAATTTGTCTATGGTCCA
CTGTCGGATCGCCTTGGCCGTAAGCCGATCATCATCGCCGGTCTATTGATTTACATTGCCGGCACTATGCTGTCACTGTT
TGCACACGAGTACACTTGGTTCCTTGTCGGTAGCTTTGTTCAAGGCTTGGGGATCGGTTGTGGTGGTGCAATGTCTCGCA
CCCTGACTCGTGACTGTTTTGAAGGCGCTGAATTACATCGCGCCAACAGTTTGATCAGCATGTGCGTGATCTTTTCACCG
CTGATGGCTCCTGTATTGGGCGGCGTGCTAACGGAAACCTTTGGCTGGCGTTCAAGCTATTTGTTTTTGGCGCTATTTGC
TATTGCGGTCATTATTACCATGATGACCAGCATGATGGAAACGTTGCCAAAAGAAAAACGTAAACACGAGCCTGTTGCTC
GCAGCTACCACTACGTTTTATCCAACAAACGTTTCCAAGGTTACTTACTTTGCTTAGTTGCAACCTTTGCTGGCGTGGCG
GTTTTTGAGGCAGCGGCGGGCGTGCTATTAGGCGGTGTGTTAGGGCTGCCTGCGACTGTCGTCAGCATTCTATTTGTATT
GCCGATCCCGGGCTATCTGATTGGTGCGGGCTTATCCAGCTACATTGCGCAGCGTCGCTCACAAAAGCGTGCTCTGAACG
TTGGCTTGGTCGCAATACTGATCGGCGCAGCCGTGGTGCTAATTCCAGGTCTGTTTGGTCAAACAACGGCACTGACGTTG
GTCGGTGGTGCGACAATTTACTTCCTTGGCGCAGGCATTTTGTTCCCAGCGGCGACCACGGGAGCACTGGCTCCTTTCCC
TTATCACGCGGGAACCGGTGGCGCTATTTTGGGCGGTATGCAAAATCTAGGAGCAGGTATCGCGACATTAATCGCCTCCT
TGTTCCCAGCCCAAGATCAACTGCCTCTTGGCTGCCTCATGCTCGCAATGTCCATCCTTGCGATGTTAGGGTTGCGTTTG
GTCTATCGTAAACCTGATTCTTCCAATGAAATGCCGCTGGCAATCTGA

Upstream 100 bases:

>100_bases
AGATCACATTTCCGGATTGATCACAACGCCATCAACGACTAATATCTCGCGCCGTAAATCCCTATAAAACTCATCATCTG
GCAGTCCTTACCGATCTGTC

Downstream 100 bases:

>100_bases
TCTTTCTAAGAGGCGATTTCATCGCCTCTTTTTTTATTTGCTCCGATAGCGATTATCCACCATCTGCAATGCGTTCAAGT
GCTTCAATTCTGCGCCACTG

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MVFFVFNLAGEFAQMSASFPLAKLTLLIAILTAVGQMTQTMYVPSIGHMASEFLVSPAALQAVMACYLIPYGLSQFVYGP
LSDRLGRKPIIIAGLLIYIAGTMLSLFAHEYTWFLVGSFVQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSP
LMAPVLGGVLTETFGWRSSYLFLALFAIAVIITMMTSMMETLPKEKRKHEPVARSYHYVLSNKRFQGYLLCLVATFAGVA
VFEAAAGVLLGGVLGLPATVVSILFVLPIPGYLIGAGLSSYIAQRRSQKRALNVGLVAILIGAAVVLIPGLFGQTTALTL
VGGATIYFLGAGILFPAATTGALAPFPYHAGTGGAILGGMQNLGAGIATLIASLFPAQDQLPLGCLMLAMSILAMLGLRL
VYRKPDSSNEMPLAI

Sequences:

>Translated_415_residues
MVFFVFNLAGEFAQMSASFPLAKLTLLIAILTAVGQMTQTMYVPSIGHMASEFLVSPAALQAVMACYLIPYGLSQFVYGP
LSDRLGRKPIIIAGLLIYIAGTMLSLFAHEYTWFLVGSFVQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSP
LMAPVLGGVLTETFGWRSSYLFLALFAIAVIITMMTSMMETLPKEKRKHEPVARSYHYVLSNKRFQGYLLCLVATFAGVA
VFEAAAGVLLGGVLGLPATVVSILFVLPIPGYLIGAGLSSYIAQRRSQKRALNVGLVAILIGAAVVLIPGLFGQTTALTL
VGGATIYFLGAGILFPAATTGALAPFPYHAGTGGAILGGMQNLGAGIATLIASLFPAQDQLPLGCLMLAMSILAMLGLRL
VYRKPDSSNEMPLAI
>Mature_415_residues
MVFFVFNLAGEFAQMSASFPLAKLTLLIAILTAVGQMTQTMYVPSIGHMASEFLVSPAALQAVMACYLIPYGLSQFVYGP
LSDRLGRKPIIIAGLLIYIAGTMLSLFAHEYTWFLVGSFVQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSP
LMAPVLGGVLTETFGWRSSYLFLALFAIAVIITMMTSMMETLPKEKRKHEPVARSYHYVLSNKRFQGYLLCLVATFAGVA
VFEAAAGVLLGGVLGLPATVVSILFVLPIPGYLIGAGLSSYIAQRRSQKRALNVGLVAILIGAAVVLIPGLFGQTTALTL
VGGATIYFLGAGILFPAATTGALAPFPYHAGTGGAILGGMQNLGAGIATLIASLFPAQDQLPLGCLMLAMSILAMLGLRL
VYRKPDSSNEMPLAI

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=346, Percent_Identity=42.1965317919075, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI48994889, Length=342, Percent_Identity=23.9766081871345, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788509, Length=356, Percent_Identity=25, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790146, Length=372, Percent_Identity=25, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787065, Length=303, Percent_Identity=26.4026402640264, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790794, Length=298, Percent_Identity=21.4765100671141, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43855; Mature: 43855

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVFFVFNLAGEFAQMSASFPLAKLTLLIAILTAVGQMTQTMYVPSIGHMASEFLVSPAAL
CEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHH
QAVMACYLIPYGLSQFVYGPLSDRLGRKPIIIAGLLIYIAGTMLSLFAHEYTWFLVGSFV
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGVLTETFGWRSSY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LFLALFAIAVIITMMTSMMETLPKEKRKHEPVARSYHYVLSNKRFQGYLLCLVATFAGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VFEAAAGVLLGGVLGLPATVVSILFVLPIPGYLIGAGLSSYIAQRRSQKRALNVGLVAIL
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAAVVLIPGLFGQTTALTLVGGATIYFLGAGILFPAATTGALAPFPYHAGTGGAILGGM
HHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCH
QNLGAGIATLIASLFPAQDQLPLGCLMLAMSILAMLGLRLVYRKPDSSNEMPLAI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]