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The map label for this gene is ycdT [H]

Identifier: 27365600

GI number: 27365600

Start: 2294116

End: 2295435

Strand: Reverse

Name: ycdT [H]

Synonym: VV1_2277

Alternate gene names: 27365600

Gene position: 2295435-2294116 (Counterclockwise)

Preceding gene: 27365601

Following gene: 27365588

Centisome position: 69.94

GC content: 41.52

Gene sequence:

>1320_bases
ATGGTCCTCTATCAATCAAAATTGTCGCCATCAACAGCAACCATAGAATTACGCAGCTTACTGATTATGATTGTGGTTAT
TTTTGTTTTTAATACACCACAAAACCTGATTAAAAGGGACAAAATAGAGAACGTACGATTACAACTGAGTGCATCATTAG
AATCTTTAAATACACGAAAGGAGTTAATACAAAGCTATCTTTCTTTAGCAGACAGTCAAATTGCTCAGCGTTATTTTTCT
GACTCAACCGACTTTATTGATCTGGTCCAAAACTTAATTCAGCATCAAAAAATCATTCGCCGAATTCGGATTATCGATAA
AAACCCAACTGAGCAAGAGACCTATTCTAAGCGTATTATCAGTTTCAATCGCTTTTACCAAAATGACTTAAACCGCCGCC
AGAGACAGACTATTTTGGATATCGAAAATGCTCTGTTTGTCGAGTTCTCACCCATTTATCAACATAACCGTTTAATGGGC
TATTTGACTGTTGAAGTCGACCTGATTCACTTTACCCCTTTGTTTCGCGACAACATGCTCCATGTTGATCTTGATGGTTT
TGTCTATTCCAGTTCGTATGCTGATATCACCGCCTTTACTTACCTCAAACATCGCGAGCAAACCTTACTTCAAGAGCTGA
ATCGTACTCACAAAACATCCGGCGTATTGGACTTTCAAGGAAAAACCTTCGTTTATCAAAACGTAGGGCAACTCAATGGC
AAAACGAGTTATTTGGTCAAAGTCATTGCCAACGAAGAACTCATCCCTAAATATTTCTACTTAATTCCACTGCTGCTTGC
CATCACGGTAGGCGCTTGTTATTACCTGTACAAGCTGAATAAGGCACAAAAGAAACTAAAAGAAATCAGCTATTTAGACC
CACTATCAGGATTAAACAATCGACACTTTCTTGCTGAAGTCGTAAAACAACAGCTTCCGCTTGAACATTATTATGCCGTT
ATGCTTGATATTGACCACTTTAAGAGTGTCAACGACCGATATGGGCATGACATCGGCGATCAGGTCATTCGCCGTGTGGC
AAAGGTGGTGAAAAGTCGTATCCGCGTCAGTGATTACGCCTTTCGTATTGGTGGTGAAGAATTTCTGTTGCTGGTCAAAA
CCCCTTCGTCCAATGAAGCACGGCAGGTTTGTGAACGCATTCGCCAAGATGTTGAGAACATGACTCAAGCGCCGCACGTC
ACGGTATCCATTGGCTTTACTGCTTTACAGACACAGCTCGACGAGACCATTCGCATGGCCGATTCACATCTGTATGAGGC
TAAGCGCAACGGGCGAAATCGTGTTTGCCCTAACGCTTAG

Upstream 100 bases:

>100_bases
TAGCGAGTGCAACAGGTAGACAGAAAGTAACTGATAAATATATCTGGAATCAATTTGCACAACCGAGGATGGGTATGTCG
GTGTTTGAGAAATAGTCAGC

Downstream 100 bases:

>100_bases
CGCCCAGCTCAACTCTCAGTCACTCATGCCTTCCACATCGCCATCTTTGACTCAAGGATGCAGATGTTTCAGCACGCCCG
CTTGATCCAGTTCATCATGC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRKELIQSYLSLADSQIAQRYFS
DSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRIISFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMG
YLTVEVDLIHFTPLFRDNMLHVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
KTSYLVKVIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNNRHFLAEVVKQQLPLEHYYAV
MLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYAFRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHV
TVSIGFTALQTQLDETIRMADSHLYEAKRNGRNRVCPNA

Sequences:

>Translated_439_residues
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRKELIQSYLSLADSQIAQRYFS
DSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRIISFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMG
YLTVEVDLIHFTPLFRDNMLHVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
KTSYLVKVIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNNRHFLAEVVKQQLPLEHYYAV
MLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYAFRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHV
TVSIGFTALQTQLDETIRMADSHLYEAKRNGRNRVCPNA
>Mature_439_residues
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRKELIQSYLSLADSQIAQRYFS
DSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRIISFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMG
YLTVEVDLIHFTPLFRDNMLHVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
KTSYLVKVIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNNRHFLAEVVKQQLPLEHYYAV
MLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYAFRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHV
TVSIGFTALQTQLDETIRMADSHLYEAKRNGRNRVCPNA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=38.5057471264368, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=36.25, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=36.3095238095238, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=35.9756097560976, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=40.7643312101911, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=30.1775147928994, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787802, Length=188, Percent_Identity=29.2553191489362, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=32.2147651006711, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=157, Percent_Identity=34.3949044585987, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=30.7692307692308, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=30.7228915662651, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=149, Percent_Identity=31.5436241610738, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=28.7425149700599, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51105; Mature: 51105

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRK
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEHHHHHHHHHHH
ELIQSYLSLADSQIAQRYFSDSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMGYLTVEVDLIHFTPLFRDNML
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCEEEEEEEEEEEEEECHHHHCCEE
HVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
EEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHCCC
KTSYLVKVIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNN
CHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RHFLAEVVKQQLPLEHYYAVMLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYA
CHHHHHHHHHHCCHHHHHHHEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHVTVSIGFTALQTQLDETIRMA
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHH
DSHLYEAKRNGRNRVCPNA
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVLYQSKLSPSTATIELRSLLIMIVVIFVFNTPQNLIKRDKIENVRLQLSASLESLNTRK
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEHHHHHHHHHHH
ELIQSYLSLADSQIAQRYFSDSTDFIDLVQNLIQHQKIIRRIRIIDKNPTEQETYSKRII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SFNRFYQNDLNRRQRQTILDIENALFVEFSPIYQHNRLMGYLTVEVDLIHFTPLFRDNML
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCEEEEEEEEEEEEEECHHHHCCEE
HVDLDGFVYSSSYADITAFTYLKHREQTLLQELNRTHKTSGVLDFQGKTFVYQNVGQLNG
EEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHCCC
KTSYLVKVIANEELIPKYFYLIPLLLAITVGACYYLYKLNKAQKKLKEISYLDPLSGLNN
CHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RHFLAEVVKQQLPLEHYYAVMLDIDHFKSVNDRYGHDIGDQVIRRVAKVVKSRIRVSDYA
CHHHHHHHHHHCCHHHHHHHEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FRIGGEEFLLLVKTPSSNEARQVCERIRQDVENMTQAPHVTVSIGFTALQTQLDETIRMA
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHH
DSHLYEAKRNGRNRVCPNA
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]