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The map label for this gene is nhaB

Identifier: 27365557

GI number: 27365557

Start: 2243579

End: 2245168

Strand: Reverse

Name: nhaB

Synonym: VV1_2232

Alternate gene names: 27365557

Gene position: 2245168-2243579 (Counterclockwise)

Preceding gene: 27365560

Following gene: 161501811

Centisome position: 68.41

GC content: 47.74

Gene sequence:

>1590_bases
ATGCCGATGTCGCTCGGAAACGCTTTTATTAAGAATTTTCTTGGTAAAGCGCCTGATTGGTATAAAGTTGCCATCATTTC
TTTTTTAATCATTAACCCGATTGTTTTCTTCTTCGTTGACCCATTTGTAGCAGGCTGGCTACTCGTCGTGGAATTTATTT
TCACGTTGGCAATGGCGCTCAAATGTTATCCTCTTCAACCTGGTGGTTTATTAGCCATTGAAGCCATCGCGATTGGCATG
ACTAGCCCTGAACAGGTTAAGCATGAGCTCGTCGCCAATATCGAAGTGTTGTTGTTACTGGTCTTCATGGTCGCTGGCAT
CTACTTCATGAAGCAGTTGCTACTCTTCATTTTCACCAAGATATTGCTTGGCATTCGCTCTAAAGCGATTTTGTCTTTAG
CGTTTTGTTTTGCCGCGGCCTTTCTTTCCGCTTTCTTAGACGCACTGACCGTTATCGCCGTTGTTATCAGCGTTGCCGTG
GGTTTCTATTCGATCTACCACAAAGTGGCATCGGGTAAGGGCGTCAGTTCCGATCATGACCATACACAAGACGATCATCT
TGCCGAGCTGACTCGTGAAGACTTAGAAAACTACCGCGCATTTTTACGCTCACTGCTCATGCATGCGGGTGTTGGTACTG
CGCTCGGTGGCGTGACGACCATGGTAGGTGAACCTCAAAACTTGATCATTGCAGACCAAGCAAGTTGGTTGTTTGGTGAG
TTCTTGATTCGCATGGCTCCAGTTACGCTTCCTGTTTTCGTTTGTGGTTTGCTCACCTGTTTTGCGGTCGAAAAACTTAA
GGTGTTTGGCTACGGCGCTGAACTACCTGATAACGTTCGTCATATTCTTGTCGAATTCGACAAAGAAGAACGCAAGGCTC
GTACCAACCAAGATGTGGCCAAACTTTGGATTCAAGGTCTTATCGCCGTATGGCTGATTGTCGGCCTCGCACTGCACTTG
GCCGCGGTAGGTTTGATCGGTTTGTCCGTCATCATCCTAGCCACAGCCTTCACTGGTGTGATTGAAGAACACTCACTCGG
TAAAGCGTTTGAAGAAGCACTGCCATTTACGGCACTTCTTGCGGTTTTCTTCTCTATCGTGGCGGTAATCATCGACCAAG
AGTTGTTTAAGCCTGTTATCGATGCAGTTCTCGCGGTGGAAGACAAAGGCACGCAGTTGGCGATGTTCTACGTGGCAAAC
GGTTTGCTTTCGATGGTGTCTGACAACGTGTTCGTCGGTACGGTTTACATCAACGAAGTCAAAACTGCGCTAGTAGAAGG
CATTATTACACGTGACCAGTTTGACTTGCTGGCCGTGGCGATCAACACAGGAACTAACTTGCCATCAGTGGCAACACCAA
ACGGCCAAGCGGCGTTCCTATTTTTGTTGACTTCAGCACTCGCACCATTGATTCGTCTGTCTTACGGCAAGATGGTGATC
ATGGCTCTGCCATACACGATTGTACTGGCTCTGGTTGGCTTGTTTGGCATCGTCTTCTTACTTGAGCCTATGACAGCTTG
GTTTTACGACGCAGGTTGGATTGCTCACCATGTGGGTGAGGCAACTCACGCAGTTTCCGGCGGACATTAA

Upstream 100 bases:

>100_bases
TAAGTTATTGTTTATAAAGATAGATATTTCTTCTTTAAAGCATAGGCTTAAGCACTATGTTGTAGTGTCTATTCATATAT
AAGTTAATAAGAGAATTATC

Downstream 100 bases:

>100_bases
ATTTTTAGTTTGCTAAAGAAACTTATCCGAGATAAAAAGCTCTGAGTATTCAGAGCTTTTTTATTGAACATACAAAGGAT
TTTAGAAGTGAATTTATTTG

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaB

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MPMSLGNAFIKNFLGKAPDWYKVAIISFLIINPIVFFFVDPFVAGWLLVVEFIFTLAMALKCYPLQPGGLLAIEAIAIGM
TSPEQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTKILLGIRSKAILSLAFCFAAAFLSAFLDALTVIAVVISVAV
GFYSIYHKVASGKGVSSDHDHTQDDHLAELTREDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQASWLFGE
FLIRMAPVTLPVFVCGLLTCFAVEKLKVFGYGAELPDNVRHILVEFDKEERKARTNQDVAKLWIQGLIAVWLIVGLALHL
AAVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALLAVFFSIVAVIIDQELFKPVIDAVLAVEDKGTQLAMFYVAN
GLLSMVSDNVFVGTVYINEVKTALVEGIITRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGKMVI
MALPYTIVLALVGLFGIVFLLEPMTAWFYDAGWIAHHVGEATHAVSGGH

Sequences:

>Translated_529_residues
MPMSLGNAFIKNFLGKAPDWYKVAIISFLIINPIVFFFVDPFVAGWLLVVEFIFTLAMALKCYPLQPGGLLAIEAIAIGM
TSPEQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTKILLGIRSKAILSLAFCFAAAFLSAFLDALTVIAVVISVAV
GFYSIYHKVASGKGVSSDHDHTQDDHLAELTREDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQASWLFGE
FLIRMAPVTLPVFVCGLLTCFAVEKLKVFGYGAELPDNVRHILVEFDKEERKARTNQDVAKLWIQGLIAVWLIVGLALHL
AAVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALLAVFFSIVAVIIDQELFKPVIDAVLAVEDKGTQLAMFYVAN
GLLSMVSDNVFVGTVYINEVKTALVEGIITRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGKMVI
MALPYTIVLALVGLFGIVFLLEPMTAWFYDAGWIAHHVGEATHAVSGGH
>Mature_528_residues
PMSLGNAFIKNFLGKAPDWYKVAIISFLIINPIVFFFVDPFVAGWLLVVEFIFTLAMALKCYPLQPGGLLAIEAIAIGMT
SPEQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTKILLGIRSKAILSLAFCFAAAFLSAFLDALTVIAVVISVAVG
FYSIYHKVASGKGVSSDHDHTQDDHLAELTREDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQASWLFGEF
LIRMAPVTLPVFVCGLLTCFAVEKLKVFGYGAELPDNVRHILVEFDKEERKARTNQDVAKLWIQGLIAVWLIVGLALHLA
AVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALLAVFFSIVAVIIDQELFKPVIDAVLAVEDKGTQLAMFYVANG
LLSMVSDNVFVGTVYINEVKTALVEGIITRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGKMVIM
ALPYTIVLALVGLFGIVFLLEPMTAWFYDAGWIAHHVGEATHAVSGGH

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3067

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaB Na(+)/H(+) (TC 2.A.34) antiporter family

Homologues:

Organism=Escherichia coli, GI1787435, Length=515, Percent_Identity=68.9320388349515, Blast_Score=683, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAB_VIBVU (Q8DAG9)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761085.1
- GeneID:   1179113
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2232
- HOGENOM:   HBG296776
- OMA:   CFLVEKF
- ProtClustDB:   PRK09547
- BioCyc:   VVUL216895:VV1_2232-MONOMER
- HAMAP:   MF_01599
- InterPro:   IPR004671
- TIGRFAMs:   TIGR00774

Pfam domain/function: PF06450 NhaB

EC number: NA

Molecular weight: Translated: 57457; Mature: 57326

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf777544)-; HASH(0xfbd0220)-; HASH(0xdd9a2bc)-; HASH(0xfbdcd54)-; HASH(0xfbd3c5c)-; HASH(0xb50b264)-; HASH(0xfbd3da0)-; HASH(0xfa25244)-; HASH(0xfbd7294)-; HASH(0xfbdb98c)-; HASH(0xb838ee8)-; HASH(0xfbd0154)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
PMSLGNAFIKNFLGKAPDWYKVAIISFLIINPIVFFFVDPFVAGWLLVVEFIFTLAMAL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCYPLQPGGLLAIEAIAIGMTSPEQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTK
HCCCCCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLGIRSKAILSLAFCFAAAFLSAFLDALTVIAVVISVAVGFYSIYHKVASGKGVSSDHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HTQDDHLAELTREDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQASWLFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHH
FLIRMAPVTLPVFVCGLLTCFAVEKLKVFGYGAELPDNVRHILVEFDKEERKARTNQDVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCHHHH
KLWIQGLIAVWLIVGLALHLAAVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AVFFSIVAVIIDQELFKPVIDAVLAVEDKGTQLAMFYVANGLLSMVSDNVFVGTVYINEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEHHHH
KTALVEGIITRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGKMVI
HHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
MALPYTIVLALVGLFGIVFLLEPMTAWFYDAGWIAHHVGEATHAVSGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3 Proton [Periplasm]; Na (I) [Cytoplasm] [C]

Specific reaction: 3 Proton [Periplasm] + 2 Na (I) [Cytoplasm] = 2 Na (I) [Periplasm] + 3 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA