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The map label for this gene is cspD [H]

Identifier: 27365451

GI number: 27365451

Start: 2121270

End: 2121500

Strand: Reverse

Name: cspD [H]

Synonym: VV1_2119

Alternate gene names: 27365451

Gene position: 2121500-2121270 (Counterclockwise)

Preceding gene: 27365455

Following gene: 27365449

Centisome position: 64.64

GC content: 46.32

Gene sequence:

>231_bases
ATGTATAGCATGGCTACAGGTACAGTAAAGTGGTTCAATAACGCCAAAGGATTTGGTTTTATCTGTTCAGATCAAGAGGA
AGGCGACATCTTCGCCCACTACTCAACGATTCAGATGGACGGTTATCGTACACTGAAAGCGGGCCAACAAGTCACGTATG
AGATTGAGAAAGGTCCGAAAGGGTGTCACGCTAGCAGCGTCGTTCCACTCGAAGTACAAGCAACGAAGTAA

Upstream 100 bases:

>100_bases
CACACGAAATTCTTAAATTTAGCTTAAAAGCAAATTTGCCATCAGTCTGTTACACAGGCTAAAGATTGGCTAACAACATC
AGTAACAAAATGCATGAGGG

Downstream 100 bases:

>100_bases
GACTGGTATTGCGACAAAATTCACTTTTAAGCAACGCTTTAGCTCTTATTGAAAAACCCGCCTTTCGGCGGGTTTTGTTT
TTTGCATTAAGCTTCAATCA

Product: cold shock domain-containing protein CspD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 76; Mature: 76

Protein sequence:

>76_residues
MYSMATGTVKWFNNAKGFGFICSDQEEGDIFAHYSTIQMDGYRTLKAGQQVTYEIEKGPKGCHASSVVPLEVQATK

Sequences:

>Translated_76_residues
MYSMATGTVKWFNNAKGFGFICSDQEEGDIFAHYSTIQMDGYRTLKAGQQVTYEIEKGPKGCHASSVVPLEVQATK
>Mature_76_residues
MYSMATGTVKWFNNAKGFGFICSDQEEGDIFAHYSTIQMDGYRTLKAGQQVTYEIEKGPKGCHASSVVPLEVQATK

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=72, Percent_Identity=70.8333333333333, Blast_Score=112, Evalue=4e-27,
Organism=Escherichia coli, GI1789979, Length=70, Percent_Identity=51.4285714285714, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=52.9411764705882, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1786841, Length=68, Percent_Identity=48.5294117647059, Blast_Score=75, Evalue=6e-16,
Organism=Escherichia coli, GI2367114, Length=70, Percent_Identity=47.1428571428571, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1787834, Length=70, Percent_Identity=48.5714285714286, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=48.4375, Blast_Score=69, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8399; Mature: 8399

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSMATGTVKWFNNAKGFGFICSDQEEGDIFAHYSTIQMDGYRTLKAGQQVTYEIEKGPK
CCCCCCCEEEECCCCCCCEEEECCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCC
GCHASSVVPLEVQATK
CCCCCCEEEEEEEECC
>Mature Secondary Structure
MYSMATGTVKWFNNAKGFGFICSDQEEGDIFAHYSTIQMDGYRTLKAGQQVTYEIEKGPK
CCCCCCCEEEECCCCCCCEEEECCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCC
GCHASSVVPLEVQATK
CCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]