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The map label for this gene is intB [H]

Identifier: 27365385

GI number: 27365385

Start: 2041144

End: 2042466

Strand: Reverse

Name: intB [H]

Synonym: VV1_2048

Alternate gene names: 27365385

Gene position: 2042466-2041144 (Counterclockwise)

Preceding gene: 27365386

Following gene: 27365382

Centisome position: 62.23

GC content: 38.55

Gene sequence:

>1323_bases
ATGTTAACTGTAAATCAAATCAAAGCGCTTCCTGCCAAAAAAACCGCTTACTACAAGCCAGACAATACAGGTGAGCGAGG
CACAGGCCGCTTAACTGTCCAAGTCACTCCTGCTGGCTCTAAGATCTTCAAGTTCAAATACCATAAAGAGAAGAAAGAAG
TTTTCATCGCTTTGGGTAAGTTCCCTGCTCTAACTTTATCTAAAGCACGTGATCTAGCTAACGAGTACAGTGAAATGCTC
TCTAAAGGATTAGATCCAAAAAATGAGCTAGAAAGGCAGTCTGAAGAGAAGAAAACAGCAGAACGTGAAAAAGATCAATT
AGGCACCTTTGAGCAACTTATTGAGTTCCATAAGCAGAACAAACAAGATAATGGAAATCGTAGTCACGAAGACGAATATC
AGCTCATAGTGAAAAATGTGTTCCCTCATCTCGACATACATCGAAAGGCGAAAGATTTTGAAACTGAAGATTTTTTGGAC
ATATTAGCAATCCCAATTGAAGAAGGATTTGTGGACAAGAGTAATAAAATTCGCTCCATTCTGCATGCGGCATTCAACTT
CGCTTTAGGGTACGAAAATAACCCTAAATTCAGAAAAAGAGAAACCAAATTCGCTCTTGACCACAATCCGATATCGAAAA
TACCAAAACAACCTGAAAAAATTGGTTCACACTTCCTATCTTGGAATGAAGTAACCCAATTACTTTATGACATGGAACAT
AGATTTGATGATTTAAACCTTGCATACCAAACCAGACAAGTGCTAAGGCTTTGTTTTTACTTGGGAGGGCAACGACCTCA
CGAAGTGGTAACGATCAAATGGTCGCACATAAATTTTGATCAAAAAACTGTTCTTGTCAGCGAAGCTTTCCAAAAAACAA
AGAAAAATCACATGCTTCCTCTGACTAACTCTGCAATCCAAATACTAGAAGAAATCAGAGAACAAACGGCTACTATTGGT
AGCCCATTTGTTTTCTTTAAGAAAACAAATTCAAATGAACATATGCCTACCAACACTATTGCTCAAGCACTGCTAACTTA
CAAAAAAGTTACAAAAATTAGGCCTTTTGTCGCTAGGGACTTTCGGAGAACAGTAAAAACATTGGGAGGGGAAATCAAGA
TTCCCAAAGAGTACCGTGATCGTATACAGGGGCATGCTATCAATGATGTATCTGGTAGGCACTATGATATGTATGAGTAC
ATTGATGAAAAACGATTGGGACTTGAACTATGGGAAAAGGCGCTCAATTCACGCCTTGAAGATTATAAGCTAGTATTTTT
TGAGAGAGAGCTTCCTTGGAAGAAAAAGAGCCTTACCTCCTAA

Upstream 100 bases:

>100_bases
TCACTTATAGTTACATTTAGCTACACTGGACTAAACAGTAACCCGACTACGTAGCCGACTACAAAATATAGCTAGTAGCG
GTTCATCCAAGGTGATCCAT

Downstream 100 bases:

>100_bases
TACAACTAATTTTATCTACGATAACTGCCAAGAAAGACTCGATAGCTCAATCACAACCAAGCCATTGCCCTTTCGAGTTA
CAGGCGTGGCTGACAACTAA

Product: Integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLTVNQIKALPAKKTAYYKPDNTGERGTGRLTVQVTPAGSKIFKFKYHKEKKEVFIALGKFPALTLSKARDLANEYSEML
SKGLDPKNELERQSEEKKTAEREKDQLGTFEQLIEFHKQNKQDNGNRSHEDEYQLIVKNVFPHLDIHRKAKDFETEDFLD
ILAIPIEEGFVDKSNKIRSILHAAFNFALGYENNPKFRKRETKFALDHNPISKIPKQPEKIGSHFLSWNEVTQLLYDMEH
RFDDLNLAYQTRQVLRLCFYLGGQRPHEVVTIKWSHINFDQKTVLVSEAFQKTKKNHMLPLTNSAIQILEEIREQTATIG
SPFVFFKKTNSNEHMPTNTIAQALLTYKKVTKIRPFVARDFRRTVKTLGGEIKIPKEYRDRIQGHAINDVSGRHYDMYEY
IDEKRLGLELWEKALNSRLEDYKLVFFERELPWKKKSLTS

Sequences:

>Translated_440_residues
MLTVNQIKALPAKKTAYYKPDNTGERGTGRLTVQVTPAGSKIFKFKYHKEKKEVFIALGKFPALTLSKARDLANEYSEML
SKGLDPKNELERQSEEKKTAEREKDQLGTFEQLIEFHKQNKQDNGNRSHEDEYQLIVKNVFPHLDIHRKAKDFETEDFLD
ILAIPIEEGFVDKSNKIRSILHAAFNFALGYENNPKFRKRETKFALDHNPISKIPKQPEKIGSHFLSWNEVTQLLYDMEH
RFDDLNLAYQTRQVLRLCFYLGGQRPHEVVTIKWSHINFDQKTVLVSEAFQKTKKNHMLPLTNSAIQILEEIREQTATIG
SPFVFFKKTNSNEHMPTNTIAQALLTYKKVTKIRPFVARDFRRTVKTLGGEIKIPKEYRDRIQGHAINDVSGRHYDMYEY
IDEKRLGLELWEKALNSRLEDYKLVFFERELPWKKKSLTS
>Mature_440_residues
MLTVNQIKALPAKKTAYYKPDNTGERGTGRLTVQVTPAGSKIFKFKYHKEKKEVFIALGKFPALTLSKARDLANEYSEML
SKGLDPKNELERQSEEKKTAEREKDQLGTFEQLIEFHKQNKQDNGNRSHEDEYQLIVKNVFPHLDIHRKAKDFETEDFLD
ILAIPIEEGFVDKSNKIRSILHAAFNFALGYENNPKFRKRETKFALDHNPISKIPKQPEKIGSHFLSWNEVTQLLYDMEH
RFDDLNLAYQTRQVLRLCFYLGGQRPHEVVTIKWSHINFDQKTVLVSEAFQKTKKNHMLPLTNSAIQILEEIREQTATIG
SPFVFFKKTNSNEHMPTNTIAQALLTYKKVTKIRPFVARDFRRTVKTLGGEIKIPKEYRDRIQGHAINDVSGRHYDMYEY
IDEKRLGLELWEKALNSRLEDYKLVFFERELPWKKKSLTS

Specific function: Integrase Is Necessary For Integration Of The Phage Into The Host Genome By Site-Specific Recombination. In Conjunction With Excisionase, Integrase Is Also Necessary For Excision Of The Prophage From The Host Genome. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=319, Percent_Identity=25.3918495297806, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 51498; Mature: 51498

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVNQIKALPAKKTAYYKPDNTGERGTGRLTVQVTPAGSKIFKFKYHKEKKEVFIALGK
CCCHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCEEEEEEHHHCCCCEEEEECC
FPALTLSKARDLANEYSEMLSKGLDPKNELERQSEEKKTAEREKDQLGTFEQLIEFHKQN
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
KQDNGNRSHEDEYQLIVKNVFPHLDIHRKAKDFETEDFLDILAIPIEEGFVDKSNKIRSI
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
LHAAFNFALGYENNPKFRKRETKFALDHNPISKIPKQPEKIGSHFLSWNEVTQLLYDMEH
HHHHHHHHCCCCCCCCHHHHHCCEECCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHH
RFDDLNLAYQTRQVLRLCFYLGGQRPHEVVTIKWSHINFDQKTVLVSEAFQKTKKNHMLP
HCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LTNSAIQILEEIREQTATIGSPFVFFKKTNSNEHMPTNTIAQALLTYKKVTKIRPFVARD
CCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FRRTVKTLGGEIKIPKEYRDRIQGHAINDVSGRHYDMYEYIDEKRLGLELWEKALNSRLE
HHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DYKLVFFERELPWKKKSLTS
CEEEEEEECCCCCCHHCCCC
>Mature Secondary Structure
MLTVNQIKALPAKKTAYYKPDNTGERGTGRLTVQVTPAGSKIFKFKYHKEKKEVFIALGK
CCCHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCEEEEEEHHHCCCCEEEEECC
FPALTLSKARDLANEYSEMLSKGLDPKNELERQSEEKKTAEREKDQLGTFEQLIEFHKQN
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
KQDNGNRSHEDEYQLIVKNVFPHLDIHRKAKDFETEDFLDILAIPIEEGFVDKSNKIRSI
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
LHAAFNFALGYENNPKFRKRETKFALDHNPISKIPKQPEKIGSHFLSWNEVTQLLYDMEH
HHHHHHHHCCCCCCCCHHHHHCCEECCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHH
RFDDLNLAYQTRQVLRLCFYLGGQRPHEVVTIKWSHINFDQKTVLVSEAFQKTKKNHMLP
HCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LTNSAIQILEEIREQTATIGSPFVFFKKTNSNEHMPTNTIAQALLTYKKVTKIRPFVARD
CCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FRRTVKTLGGEIKIPKEYRDRIQGHAINDVSGRHYDMYEYIDEKRLGLELWEKALNSRLE
HHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DYKLVFFERELPWKKKSLTS
CEEEEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]