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The map label for this gene is rlmA [H]

Identifier: 27365362

GI number: 27365362

Start: 2013538

End: 2014362

Strand: Reverse

Name: rlmA [H]

Synonym: VV1_2021

Alternate gene names: 27365362

Gene position: 2014362-2013538 (Counterclockwise)

Preceding gene: 27365363

Following gene: 326423923

Centisome position: 61.38

GC content: 50.67

Gene sequence:

>825_bases
ATGGCGTATCAATGCCCCTTGTGTCACCTTGCGTTAGAGCAAGCAGAGCGCACGTTCAAATGTTCCAATAATCATCAATT
TGACCTGGCCAAAGAAGGTTACGTTAACTTGATGCCTGCCCATCACAAACGTTCGAAAGATCCCGGTGACAACAAAGAGA
TGATGCAGGCTCGCCGCCGCTTCCTTGAAGGCAACCATTACGACCCAATGCGTCAAAAAGTGGCGCAACTTTGCCGCCAA
TACCTGCAAGGTACGCCGCACCGCCTGCTCGATATTGGTTGTGGTGAAGGTTACTACACCGATGAAGTCGCCAAAACCTT
GGTCGCGCAGCACCCAGAAGCCAAAACATTTGGTTTGGACATTTCCAAAATCGCCGTTCGTTTCGCGGCAAAACGCTACC
CTAATGTTAAGTTTTGTGTCGCTTCCAGCCATCGACTTCCCTTTGCAGACGAAGCGTTGGACGGCATCCTCCGCATTTAC
GCACCATGCAAGGCCGAAGAGCTGGCGCGATGCATTGCCGACAATGGCGTTGTGATCACGGTAACGCCTGCTGGCCGCCA
CTTACTGCAACTGAAAGAGAAGATTTACGCCGAGCTCAAACTGCATAGCGAAGAACCAGAGCAAATGGATGGATTTACGC
TAGAGCACCAAGAAAAGCTCAATTACGTAATGGACTTAGCAGGCCAAGATGCGTTTGACTTACTGCAAATGACCCCCTTT
GCTTGGAAAGCCAGCGATGCCCTTCGCGACGAGCTAAAGTCCGCTACACTGTTCCAGTGTGAGGCCGATTTCATGTTGCG
TGTTTACCGCAAAACAGCAAAATAG

Upstream 100 bases:

>100_bases
CGGCGGGTAAAATCTTGGCCTGCCGAGACTCAAACTGGTTAAAACAGGCGGTTCCTGTTAGACTTTGCCGCCTGTTTCAT
CGCCCCTTTTTGAGGTCTTC

Downstream 100 bases:

>100_bases
CCAGACAAAACAATAAAGAGAATTATTCTCATTATTGTTGAAGTTCTAGACCGCCTCCATTACACTCTGCGACGTTTTTA
ATGGAGGCAGTATTATGCGA

Product: 23S rRNA methyltransferase A

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAYQCPLCHLALEQAERTFKCSNNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRRFLEGNHYDPMRQKVAQLCRQ
YLQGTPHRLLDIGCGEGYYTDEVAKTLVAQHPEAKTFGLDISKIAVRFAAKRYPNVKFCVASSHRLPFADEALDGILRIY
APCKAEELARCIADNGVVITVTPAGRHLLQLKEKIYAELKLHSEEPEQMDGFTLEHQEKLNYVMDLAGQDAFDLLQMTPF
AWKASDALRDELKSATLFQCEADFMLRVYRKTAK

Sequences:

>Translated_274_residues
MAYQCPLCHLALEQAERTFKCSNNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRRFLEGNHYDPMRQKVAQLCRQ
YLQGTPHRLLDIGCGEGYYTDEVAKTLVAQHPEAKTFGLDISKIAVRFAAKRYPNVKFCVASSHRLPFADEALDGILRIY
APCKAEELARCIADNGVVITVTPAGRHLLQLKEKIYAELKLHSEEPEQMDGFTLEHQEKLNYVMDLAGQDAFDLLQMTPF
AWKASDALRDELKSATLFQCEADFMLRVYRKTAK
>Mature_273_residues
AYQCPLCHLALEQAERTFKCSNNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRRFLEGNHYDPMRQKVAQLCRQY
LQGTPHRLLDIGCGEGYYTDEVAKTLVAQHPEAKTFGLDISKIAVRFAAKRYPNVKFCVASSHRLPFADEALDGILRIYA
PCKAEELARCIADNGVVITVTPAGRHLLQLKEKIYAELKLHSEEPEQMDGFTLEHQEKLNYVMDLAGQDAFDLLQMTPFA
WKASDALRDELKSATLFQCEADFMLRVYRKTAK

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=272, Percent_Identity=52.9411764705882, Blast_Score=313, Evalue=7e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 31313; Mature: 31182

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQCPLCHLALEQAERTFKCSNNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRR
CCCCCHHHHHHHHHHHHHEECCCCCCEEECCCCCEECCCCHHCCCCCCCCCHHHHHHHHH
FLEGNHYDPMRQKVAQLCRQYLQGTPHRLLDIGCGEGYYTDEVAKTLVAQHPEAKTFGLD
HHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHCCC
ISKIAVRFAAKRYPNVKFCVASSHRLPFADEALDGILRIYAPCKAEELARCIADNGVVIT
HHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEE
VTPAGRHLLQLKEKIYAELKLHSEEPEQMDGFTLEHQEKLNYVMDLAGQDAFDLLQMTPF
ECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCC
AWKASDALRDELKSATLFQCEADFMLRVYRKTAK
CCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
AYQCPLCHLALEQAERTFKCSNNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRR
CCCCHHHHHHHHHHHHHEECCCCCCEEECCCCCEECCCCHHCCCCCCCCCHHHHHHHHH
FLEGNHYDPMRQKVAQLCRQYLQGTPHRLLDIGCGEGYYTDEVAKTLVAQHPEAKTFGLD
HHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHCCC
ISKIAVRFAAKRYPNVKFCVASSHRLPFADEALDGILRIYAPCKAEELARCIADNGVVIT
HHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEE
VTPAGRHLLQLKEKIYAELKLHSEEPEQMDGFTLEHQEKLNYVMDLAGQDAFDLLQMTPF
ECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCC
AWKASDALRDELKSATLFQCEADFMLRVYRKTAK
CCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]