Click here to switch to the map view.

The map label for this gene is gloA [H]

Identifier: 27365357

GI number: 27365357

Start: 2009079

End: 2009474

Strand: Reverse

Name: gloA [H]

Synonym: VV1_2016

Alternate gene names: 27365357

Gene position: 2009474-2009079 (Counterclockwise)

Preceding gene: 27365358

Following gene: 27365356

Centisome position: 61.23

GC content: 47.98

Gene sequence:

>396_bases
ATGACCAAAATGATTCACAGCATGATCCGTGTCAGCGATCTCGACAGATCGATCGCTTTCTACCGTGATGCACTAGAACT
CGAAATCAAAGACCAATTTGTTTTCGATACTTTCACTCTGACTTACCTCGCCAACAAAACCACCGGCTTCGAGCTTGAGT
TGACTTACAATCACGGCATGAGTGATCCCTACACACATGGGACAGGCTACGGCCACCTTGCAGTGTGCGTCGAAGACATC
GAACTCGCTCATAAACGCCTACGCGATTTAGGGATAGCACCCAGTGATGTGAAAGCGATGGATCACAACCAAAAACACCT
TGCGACCTTCTTCTTTATCACCGACCCAGACGGATACAAAATCGAGTTTTTGCAGCGCCAAGGCCGCTACCAATAA

Upstream 100 bases:

>100_bases
GCCAAGAGCTCGTAAATAGACAAATTTAAATGTAATACTCACGTACTACCAAAGGTTGGTTTCTCAACATTATTCTGTCG
CCTCGCAATGAGGAACCACC

Downstream 100 bases:

>100_bases
GCATCACTCGAATACGATTTTCGGCTTGAGTTTAGGGTTAGACATTATTTAAAAAACTCATTAAGGGGCCGTCATTGTTA
GCAATGACCTGGTCTCCTGT

Product: Lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MTKMIHSMIRVSDLDRSIAFYRDALELEIKDQFVFDTFTLTYLANKTTGFELELTYNHGMSDPYTHGTGYGHLAVCVEDI
ELAHKRLRDLGIAPSDVKAMDHNQKHLATFFFITDPDGYKIEFLQRQGRYQ

Sequences:

>Translated_131_residues
MTKMIHSMIRVSDLDRSIAFYRDALELEIKDQFVFDTFTLTYLANKTTGFELELTYNHGMSDPYTHGTGYGHLAVCVEDI
ELAHKRLRDLGIAPSDVKAMDHNQKHLATFFFITDPDGYKIEFLQRQGRYQ
>Mature_130_residues
TKMIHSMIRVSDLDRSIAFYRDALELEIKDQFVFDTFTLTYLANKTTGFELELTYNHGMSDPYTHGTGYGHLAVCVEDIE
LAHKRLRDLGIAPSDVKAMDHNQKHLATFFFITDPDGYKIEFLQRQGRYQ

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Escherichia coli, GI1787940, Length=130, Percent_Identity=31.5384615384615, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15198; Mature: 15067

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMIHSMIRVSDLDRSIAFYRDALELEIKDQFVFDTFTLTYLANKTTGFELELTYNHGM
CHHHHHHHHHHHHCHHHHHHHHHHEEEEECCEEEEHHEEEEEEECCCCCEEEEEEECCCC
SDPYTHGTGYGHLAVCVEDIELAHKRLRDLGIAPSDVKAMDHNQKHLATFFFITDPDGYK
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEEEEECCCCCE
IEFLQRQGRYQ
EEEEECCCCCC
>Mature Secondary Structure 
TKMIHSMIRVSDLDRSIAFYRDALELEIKDQFVFDTFTLTYLANKTTGFELELTYNHGM
HHHHHHHHHHHHCHHHHHHHHHHEEEEECCEEEEHHEEEEEEECCCCCEEEEEEECCCC
SDPYTHGTGYGHLAVCVEDIELAHKRLRDLGIAPSDVKAMDHNQKHLATFFFITDPDGYK
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEEEEECCCCCE
IEFLQRQGRYQ
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]