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The map label for this gene is purN [H]

Identifier: 27365245

GI number: 27365245

Start: 1894661

End: 1895299

Strand: Reverse

Name: purN [H]

Synonym: VV1_1899

Alternate gene names: 27365245

Gene position: 1895299-1894661 (Counterclockwise)

Preceding gene: 27365246

Following gene: 27365242

Centisome position: 57.75

GC content: 49.3

Gene sequence:

>639_bases
ATGAAAAAAATCGTGGTACTGATTTCCGGCAGCGGCAGCAACCTGCAAGCCATTCTTGAAGCATGCGAGTGTGATACAAG
CCGAGCCAAAGTGTGTGCGGTTTTCTCAAACAAAGCCGACGCTTACGGTCTTGAACGAGCCAAACAGTTTTCGGTACCCG
CTCACTACATCGATCCCAAAGCTTTTACCGATAGAGAAAGTTTTGATCGTGAATTGATGAAAGCGATAGACGAATACCAA
CCCGACATCGTAGTACTGGCTGGTTACATGCGCATTCTGAGTGGTGAATTTGTACGTCACTATTTAGGAAAAATGGTCAA
TATACATCCTTCCCTCTTGCCTAAATACCCCGGCCTTCATACCCACCAGCGCGCTATCGATGCCGGGGACAGTGAACACG
GCACCAGTGTGCATTTTGTCACGGAAGAATTAGATGGGGGCCCAGTGATCTTACAGGCGAAAGTGCCTGTTTTCGCAGAA
GATGACGCACAAAGCCTAGCGGAACGTGTGTTAACGCAAGAGCACAGCATCTATCCCTTAGTGGTGAAATGGATGGCAGA
AGAGCGGCTTGTCATGCAACAAGGTGTTGCCTATCTCGATGGCCAACCACTTGGCAGCGCCGGTTACGCTGCAGAGTAA

Upstream 100 bases:

>100_bases
GTCCTCATTCTGCTATTTGGCACACTGAAAACCCTGCAAAAAGCAGGTATTTTCTTGCAAGACAGTGATCTTAAGAACCA
GCAGTAAATGGACAGTGGTT

Downstream 100 bases:

>100_bases
GGCGATTCATTGTCGCCACTCGACATCGAAAGAAAAAATGGGGTTGCTTATCGCAACCCATTTTCAATACAAACTCGCAA
TACCGTTTTATTCAAGTGGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKKIVVLISGSGSNLQAILEACECDTSRAKVCAVFSNKADAYGLERAKQFSVPAHYIDPKAFTDRESFDRELMKAIDEYQ
PDIVVLAGYMRILSGEFVRHYLGKMVNIHPSLLPKYPGLHTHQRAIDAGDSEHGTSVHFVTEELDGGPVILQAKVPVFAE
DDAQSLAERVLTQEHSIYPLVVKWMAEERLVMQQGVAYLDGQPLGSAGYAAE

Sequences:

>Translated_212_residues
MKKIVVLISGSGSNLQAILEACECDTSRAKVCAVFSNKADAYGLERAKQFSVPAHYIDPKAFTDRESFDRELMKAIDEYQ
PDIVVLAGYMRILSGEFVRHYLGKMVNIHPSLLPKYPGLHTHQRAIDAGDSEHGTSVHFVTEELDGGPVILQAKVPVFAE
DDAQSLAERVLTQEHSIYPLVVKWMAEERLVMQQGVAYLDGQPLGSAGYAAE
>Mature_212_residues
MKKIVVLISGSGSNLQAILEACECDTSRAKVCAVFSNKADAYGLERAKQFSVPAHYIDPKAFTDRESFDRELMKAIDEYQ
PDIVVLAGYMRILSGEFVRHYLGKMVNIHPSLLPKYPGLHTHQRAIDAGDSEHGTSVHFVTEELDGGPVILQAKVPVFAE
DDAQSLAERVLTQEHSIYPLVVKWMAEERLVMQQGVAYLDGQPLGSAGYAAE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=44.0217391304348, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=44.0217391304348, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=44.0217391304348, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788846, Length=210, Percent_Identity=63.8095238095238, Blast_Score=285, Evalue=1e-78,
Organism=Escherichia coli, GI1787483, Length=156, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=36.1581920903955, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=30.8080808080808, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582400, Length=181, Percent_Identity=47.5138121546961, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23393; Mature: 23393

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVVLISGSGSNLQAILEACECDTSRAKVCAVFSNKADAYGLERAKQFSVPAHYIDPK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCC
AFTDRESFDRELMKAIDEYQPDIVVLAGYMRILSGEFVRHYLGKMVNIHPSLLPKYPGLH
CCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
THQRAIDAGDSEHGTSVHFVTEELDGGPVILQAKVPVFAEDDAQSLAERVLTQEHSIYPL
CHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCHHH
VVKWMAEERLVMQQGVAYLDGQPLGSAGYAAE
HHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIVVLISGSGSNLQAILEACECDTSRAKVCAVFSNKADAYGLERAKQFSVPAHYIDPK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCC
AFTDRESFDRELMKAIDEYQPDIVVLAGYMRILSGEFVRHYLGKMVNIHPSLLPKYPGLH
CCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
THQRAIDAGDSEHGTSVHFVTEELDGGPVILQAKVPVFAEDDAQSLAERVLTQEHSIYPL
CHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCHHH
VVKWMAEERLVMQQGVAYLDGQPLGSAGYAAE
HHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]