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The map label for this gene is gcvA [H]

Identifier: 27365099

GI number: 27365099

Start: 1728812

End: 1729735

Strand: Reverse

Name: gcvA [H]

Synonym: VV1_1740

Alternate gene names: 27365099

Gene position: 1729735-1728812 (Counterclockwise)

Preceding gene: 27365100

Following gene: 27365091

Centisome position: 52.71

GC content: 45.45

Gene sequence:

>924_bases
GTGAAAGAGCGTATGCCACCTTTGCAAGGTCTTTATTACTTTTACGTTGCATGTCAAACAGGGAGTTTTAAACTCGCAGC
TGAACAACTATTTGTGTCTGCGGCTGCAGTGAGCCAGCAAATAAGACAGCTAGAAGAATCATTGAACATCCCCCTTTTTA
TCCGACAACATCGAAAAGTCACCCTCACTCCAGAAGGAAAAGTGCTTTTCGAGCAAGCTCAAAAAGGGTTTGCTCATCTT
CAAGAAGGCGTCCGTTTGATCAGCTCGGATGCTAATCCCAACCAACTTTCCGTCTCCACCCTACCTTCTTTCGCTCAACA
CTGGTTAGTCCCTAGGCTGACTGCATTTAAGAAACAGCAAAATGAGATTTCGCTGCTACTAGAACCCACGAATCAACTCG
TCACCTTTGAAGGCTCCAAGATCGATTTAAGTATTCGATACGGGCGCGGAAAATACCCCAATATAGAAACAAAGTGGTTA
ATGGACGATCTCGTGTACCCAGTCTGTCACCCAATCTACGCACAACAGCATCACTTGCACGATTTAAGTGACATTACGAG
AGCGGATTTAATTGAAGATACTTGGCCTGATATGGACTGGGAAACGTTTATGAGAAGTGTAGGTTTAACGGGCGGCCATT
CCACCATTCAATATGACGGATCTCATTTTGTGTTGGAAGGCGCTCTTGCCGTTCAGGGCGTGGCGCTCGTCAAACATTCA
TTGGCTGCACGCTATATCCAAGAAGGCAAACTGGTCCGCATCGGAGATATTGCGATGAAAACCCGCTATAGCTACTACAT
CTGTGCCCCTTCGAGTTACTTCCAACGCAAGAAAGTAAAGCTCTTTCATCAGTGGCTACTTATGGAAATTGAAGGTTTTC
AAGACACCTATCCCATTACCGGTGAACTGGTTGAAGAGCAATAG

Upstream 100 bases:

>100_bases
TAACTTCACCTTGCTTTGTTTGTCTTTCAAGGCTAATAATCTCTCTATCAATACAAAGCCTCAAACGATACAAATTGACA
TTGAGTTAAGGAAAACTTAA

Downstream 100 bases:

>100_bases
AGACCGTTCAGATTAATGGTCACTCGCTGATCGTTACTAGAAACTGATCGTCACTAAAGACACACCGTTGCTAAAGGTTG
ATAGTTATTAAAGGCGAAGA

Product: glycine cleavage system transcription activator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKERMPPLQGLYYFYVACQTGSFKLAAEQLFVSAAAVSQQIRQLEESLNIPLFIRQHRKVTLTPEGKVLFEQAQKGFAHL
QEGVRLISSDANPNQLSVSTLPSFAQHWLVPRLTAFKKQQNEISLLLEPTNQLVTFEGSKIDLSIRYGRGKYPNIETKWL
MDDLVYPVCHPIYAQQHHLHDLSDITRADLIEDTWPDMDWETFMRSVGLTGGHSTIQYDGSHFVLEGALAVQGVALVKHS
LAARYIQEGKLVRIGDIAMKTRYSYYICAPSSYFQRKKVKLFHQWLLMEIEGFQDTYPITGELVEEQ

Sequences:

>Translated_307_residues
MKERMPPLQGLYYFYVACQTGSFKLAAEQLFVSAAAVSQQIRQLEESLNIPLFIRQHRKVTLTPEGKVLFEQAQKGFAHL
QEGVRLISSDANPNQLSVSTLPSFAQHWLVPRLTAFKKQQNEISLLLEPTNQLVTFEGSKIDLSIRYGRGKYPNIETKWL
MDDLVYPVCHPIYAQQHHLHDLSDITRADLIEDTWPDMDWETFMRSVGLTGGHSTIQYDGSHFVLEGALAVQGVALVKHS
LAARYIQEGKLVRIGDIAMKTRYSYYICAPSSYFQRKKVKLFHQWLLMEIEGFQDTYPITGELVEEQ
>Mature_307_residues
MKERMPPLQGLYYFYVACQTGSFKLAAEQLFVSAAAVSQQIRQLEESLNIPLFIRQHRKVTLTPEGKVLFEQAQKGFAHL
QEGVRLISSDANPNQLSVSTLPSFAQHWLVPRLTAFKKQQNEISLLLEPTNQLVTFEGSKIDLSIRYGRGKYPNIETKWL
MDDLVYPVCHPIYAQQHHLHDLSDITRADLIEDTWPDMDWETFMRSVGLTGGHSTIQYDGSHFVLEGALAVQGVALVKHS
LAARYIQEGKLVRIGDIAMKTRYSYYICAPSSYFQRKKVKLFHQWLLMEIEGFQDTYPITGELVEEQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=302, Percent_Identity=33.4437086092715, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=32.7526132404181, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=32.9931972789116, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI157672245, Length=183, Percent_Identity=31.6939890710383, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=27.9693486590038, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786401, Length=169, Percent_Identity=25.4437869822485, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=23.1034482758621, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=178, Percent_Identity=30.3370786516854, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35155; Mature: 35155

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERMPPLQGLYYFYVACQTGSFKLAAEQLFVSAAAVSQQIRQLEESLNIPLFIRQHRKV
CCCCCCCHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
TLTPEGKVLFEQAQKGFAHLQEGVRLISSDANPNQLSVSTLPSFAQHWLVPRLTAFKKQQ
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
NEISLLLEPTNQLVTFEGSKIDLSIRYGRGKYPNIETKWLMDDLVYPVCHPIYAQQHHLH
CCEEEEEECCCCEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLSDITRADLIEDTWPDMDWETFMRSVGLTGGHSTIQYDGSHFVLEGALAVQGVALVKHS
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCEEEEECHHHHHHHHHHHHH
LAARYIQEGKLVRIGDIAMKTRYSYYICAPSSYFQRKKVKLFHQWLLMEIEGFQDTYPIT
HHHHHHHCCCEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GELVEEQ
CCCCCCC
>Mature Secondary Structure
MKERMPPLQGLYYFYVACQTGSFKLAAEQLFVSAAAVSQQIRQLEESLNIPLFIRQHRKV
CCCCCCCHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
TLTPEGKVLFEQAQKGFAHLQEGVRLISSDANPNQLSVSTLPSFAQHWLVPRLTAFKKQQ
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
NEISLLLEPTNQLVTFEGSKIDLSIRYGRGKYPNIETKWLMDDLVYPVCHPIYAQQHHLH
CCEEEEEECCCCEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLSDITRADLIEDTWPDMDWETFMRSVGLTGGHSTIQYDGSHFVLEGALAVQGVALVKHS
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCEEEEECHHHHHHHHHHHHH
LAARYIQEGKLVRIGDIAMKTRYSYYICAPSSYFQRKKVKLFHQWLLMEIEGFQDTYPIT
HHHHHHHCCCEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GELVEEQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]