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The map label for this gene is argF

Identifier: 27364850

GI number: 27364850

Start: 1452912

End: 1453916

Strand: Reverse

Name: argF

Synonym: VV1_1466

Alternate gene names: 27364850

Gene position: 1453916-1452912 (Counterclockwise)

Preceding gene: 27364851

Following gene: 27364849

Centisome position: 44.3

GC content: 48.66

Gene sequence:

>1005_bases
ATGGCATTTAATCTCCGGAATCGCAACTTCCTCAAGTTGTTGGACTTCTCGACAAAAGAGATCCAGTTTTTGATCGATCT
GTCTGCTGATCTAAAGAAAGCCAAATACGCTGGCACTGAGCAGAAGAAACTACTGGGCAAAAACATTGCATTGATCTTTG
AGAAAGCGTCAACTCGTACCCGATGTGCTTTTGAAGTCGCCGCCTTTGATCAAGGCGCACAAGTCACCTATATCGGCCCT
TCTGGCTCGCAAATTGGTGATAAAGAGTCGATGAAAGACACTGCGCGAGTGTTGGGTCGCATGTACGATGGCATTCAATA
TCGTGGCTTTGGTCAAGCGATCGTCGAAGAGCTCGGTGCTTTCGCTGGCGTCCCGGTATGGAATGGCTTAACCGACGAAT
TTCATCCCACACAAATTCTTGCTGACTTCCTCACCATGCTTGAACACAGCCAAGGCAAAGCACTTGCCGACATTCAATTC
GCCTACCTTGGTGATGCGCGTAATAACGTCGGCAATTCGTTGATGGTGGGCGCAGCCAAAATGGGCATGGATATTCGCCT
TGTTGGCCCGCAAGCTTACTGGCCCGATGAAGAACTCGTGGCCGCCTGCCAAGCCATTGCCAAACAAACCGGCGGCAAGA
TTACCCTGACTGAAAATGTCGCAGAAGGGGTACAAGGCTGTGATTTCCTCTATACCGATGTTTGGGTCTCCATGGGTGAA
TCACCAGAGGCTTGGGATGAGCGCGTGGCATTGATGAAACCTTATCAAGTCAACATGAATGTCCTCAAGCAAACTGGCAA
TCCGAATGTCAAATTTATGCATTGCCTACCGGCTTTCCACAACGACGAAACCACCATTGGCAAACAAGTGGCTGACAAGT
TTGGTATGAAAGGCTTGGAAGTGACCGAAGAGGTGTTTGAATCTGAGCATTCGATTGTGTTTGATGAAGCCGAAAATCGC
ATGCACACCATCAAAGCCGTGATGGTCGCAACTTTAGGCAGTTAA

Upstream 100 bases:

>100_bases
AAGTTGGCTTTTTTATGCTTAATTTTAAAATAATTATTCACAAAAGTAGCATTTCTATGTTTTACTAGTGCCATGACGAT
GGATAAATAAGGAACGAGTC

Downstream 100 bases:

>100_bases
TCGATTACATTTCAGACCTAAAGCTTGATGTAATCGCTTGCGCTCGCTTTTCTTACGCGTATAATGCTCGGCAATTTGTC
CGAGGGTTGGTAAATAACAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAFNLRNRNFLKLLDFSTKEIQFLIDLSADLKKAKYAGTEQKKLLGKNIALIFEKASTRTRCAFEVAAFDQGAQVTYIGP
SGSQIGDKESMKDTARVLGRMYDGIQYRGFGQAIVEELGAFAGVPVWNGLTDEFHPTQILADFLTMLEHSQGKALADIQF
AYLGDARNNVGNSLMVGAAKMGMDIRLVGPQAYWPDEELVAACQAIAKQTGGKITLTENVAEGVQGCDFLYTDVWVSMGE
SPEAWDERVALMKPYQVNMNVLKQTGNPNVKFMHCLPAFHNDETTIGKQVADKFGMKGLEVTEEVFESEHSIVFDEAENR
MHTIKAVMVATLGS

Sequences:

>Translated_334_residues
MAFNLRNRNFLKLLDFSTKEIQFLIDLSADLKKAKYAGTEQKKLLGKNIALIFEKASTRTRCAFEVAAFDQGAQVTYIGP
SGSQIGDKESMKDTARVLGRMYDGIQYRGFGQAIVEELGAFAGVPVWNGLTDEFHPTQILADFLTMLEHSQGKALADIQF
AYLGDARNNVGNSLMVGAAKMGMDIRLVGPQAYWPDEELVAACQAIAKQTGGKITLTENVAEGVQGCDFLYTDVWVSMGE
SPEAWDERVALMKPYQVNMNVLKQTGNPNVKFMHCLPAFHNDETTIGKQVADKFGMKGLEVTEEVFESEHSIVFDEAENR
MHTIKAVMVATLGS
>Mature_333_residues
AFNLRNRNFLKLLDFSTKEIQFLIDLSADLKKAKYAGTEQKKLLGKNIALIFEKASTRTRCAFEVAAFDQGAQVTYIGPS
GSQIGDKESMKDTARVLGRMYDGIQYRGFGQAIVEELGAFAGVPVWNGLTDEFHPTQILADFLTMLEHSQGKALADIQFA
YLGDARNNVGNSLMVGAAKMGMDIRLVGPQAYWPDEELVAACQAIAKQTGGKITLTENVAEGVQGCDFLYTDVWVSMGES
PEAWDERVALMKPYQVNMNVLKQTGNPNVKFMHCLPAFHNDETTIGKQVADKFGMKGLEVTEEVFESEHSIVFDEAENRM
HTIKAVMVATLGS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=329, Percent_Identity=37.9939209726444, Blast_Score=210, Evalue=1e-54,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=67.5840978593272, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=66.7673716012085, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI48994908, Length=378, Percent_Identity=24.6031746031746, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=32.6219512195122, Blast_Score=165, Evalue=8e-42,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_VIBVU (Q8DCF5)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760378.1
- ProteinModelPortal:   Q8DCF5
- SMR:   Q8DCF5
- GeneID:   1178380
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1466
- HOGENOM:   HBG579429
- OMA:   MTPYQIN
- ProtClustDB:   PRK02102
- BioCyc:   VVUL216895:VV1_1466-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 36860; Mature: 36728

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 108-108 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNLRNRNFLKLLDFSTKEIQFLIDLSADLKKAKYAGTEQKKLLGKNIALIFEKASTRT
CCCCCCCCCEEEEECCCCCCEEEEEECCHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCC
RCAFEVAAFDQGAQVTYIGPSGSQIGDKESMKDTARVLGRMYDGIQYRGFGQAIVEELGA
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHH
FAGVPVWNGLTDEFHPTQILADFLTMLEHSQGKALADIQFAYLGDARNNVGNSLMVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCEEEEEHHH
MGMDIRLVGPQAYWPDEELVAACQAIAKQTGGKITLTENVAEGVQGCDFLYTDVWVSMGE
HCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCC
SPEAWDERVALMKPYQVNMNVLKQTGNPNVKFMHCLPAFHNDETTIGKQVADKFGMKGLE
CCCHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHH
VTEEVFESEHSIVFDEAENRMHTIKAVMVATLGS
HHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFNLRNRNFLKLLDFSTKEIQFLIDLSADLKKAKYAGTEQKKLLGKNIALIFEKASTRT
CCCCCCCCEEEEECCCCCCEEEEEECCHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCC
RCAFEVAAFDQGAQVTYIGPSGSQIGDKESMKDTARVLGRMYDGIQYRGFGQAIVEELGA
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHH
FAGVPVWNGLTDEFHPTQILADFLTMLEHSQGKALADIQFAYLGDARNNVGNSLMVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCEEEEEHHH
MGMDIRLVGPQAYWPDEELVAACQAIAKQTGGKITLTENVAEGVQGCDFLYTDVWVSMGE
HCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCC
SPEAWDERVALMKPYQVNMNVLKQTGNPNVKFMHCLPAFHNDETTIGKQVADKFGMKGLE
CCCHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHH
VTEEVFESEHSIVFDEAENRMHTIKAVMVATLGS
HHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA