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The map label for this gene is priA [H]

Identifier: 27364755

GI number: 27364755

Start: 1337926

End: 1340130

Strand: Reverse

Name: priA [H]

Synonym: VV1_1359

Alternate gene names: 27364755

Gene position: 1340130-1337926 (Counterclockwise)

Preceding gene: 27364758

Following gene: 27364754

Centisome position: 40.83

GC content: 48.8

Gene sequence:

>2205_bases
ATGCGTCCATCCATTGCTCGTGTTGCGCTGCCAGTGCCACTCGATAAACAATTTGATTATCTCGTTCCTGGACACCTTTT
TCCGATCATTGGTGGTCGCGTGAGTGTGCCATTTGGACGACAAACCTTGACTGGCATTGTCACCGCTTTGGTTCAAGAAT
CAGACTTTCCACGCGAGCAACTTAAACCCATTAAAGCGGTGTTGGATCATCGTCCTATTTGGCCCGATTCCGTCTACAGT
TTATTACTTTGGTGTAGCCACTTTTACCAATATCCCTTAGGTGAAACACTGCATAACGCCCTCCCCGCGGCACTAAGAAA
AGGCAAAGCGGCTGACTTTGCCACTCTACAAGAATGGCAACTGACCGAGTTAGGCAAAAACCAGTTGATGCAAGGGTTAA
ACAGTCGTGCGATAAAGCAAGCGAAAGTATTAAATATGCTTGCTAATGGCCCTCAGCCTCACCAAGCCTTTTTGGATGAG
GAAGTGGCATCCACAACCTTACGCACCTTAGAAGAAAAAGGCTGGATCAGCTGTGAAGAAAAAACGCCACAGTTAACTCG
GTGGGGTAACCCGATTGAACATCCCGTCGATAAACCGAAACTCAACCAAGAGCAAGCCATTGCCATTGCGACCGTTAACA
GCCAGACAGACTTTGGCTGTTATCTGTTGGAAGGGGTCACAGGTTCAGGCAAAACCGAAGTTTATCTCAACTTGATCAAA
CCCATTTTGGAACAAGGCAAACAGGCTCTGGTGTTGGTCCCTGAAATTGGTTTAACACCACAAACCATCAATCGTTTTCG
TCAACGGTTTGATGTGCCAGTCGCCGTCATCCATTCTGGTCTTAATGATACGGAACGTCTCAACGCTTGGCTTTCCGCAC
GAGATAAAGCGGCGGGCATTGTCATTGGTACACGCTCTGCACTACTGATCCCATTTGCCCAGCTAGGGATCATCATTGTC
GATGAAGAGCACGACGCTTCGTACAAACAGCAAGATAGCTTGCGTTACCATGCTCGCGATGTGGCAGTGATGCGAGCGGC
AAAAGAAGGCATTCCAATTGTTCTCGGTTCAGCTACGCCCGCGTTAGAAACCTTACACAACGCACTTACGGGCAAATATC
ATCATCTCTCGCTAACCCAACGAGCAGGAGTGGCCGTGCCAACCACCAACAAAGTGCTGGATGTCAAAGGGCTCTATCTG
GAAAGCGGCCTATCAGCGCCACTCATTGCTGAAATGCGTCGTCATTTAAAAGCGGGCAATCAGGTGATGCTGTTTCTCAA
CCGTCGAGGCTTCTCCCCTGCGTTAATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGCTGCGATGCCTATTACA
CTTATCACCAGTACAGCAATGAAATCCGCTGTCACCACTGTGGTTCTCAACAACCTATTATCCATCAATGCCAAGGTTGT
GGTTCGACACAACTGGTGACGGTTGGTGTCGGCACTGAGCAACTGGAAAACCAGTTAACGACCCTCTTTCCTGACTACAA
AGCAATTCGCATCGACCGCGATAGCACACGACGCAAAGGTAGCCTCGAAAGCGCTTTAGAAGCCATTCGCAAAGGGGAAT
ACCAAATTTTAATTGGCACGCAAATGCTGGCTAAAGGCCATCATTTCCCAGACGTGACGTTAGTGGCGCTATTGGATGTT
GATGGGTCACTGTACAGCAGTGACTTCCGAGCGTCCGAGCGATTGGCTCAGTTGTTTATTCAAGTAGCCGGACGAGCAGG
GCGTGCTAGCAAACCTGGCGAAGTGATATTACAAACCCACCATCCAGAACACACCTTGCTCAAAGCTCTGTTGACCAAGG
ATTACCGGCATTTTGCGCAGTCTGCACTGGAAGAACGGAAAATTGCCCAACTTCCCCCGTTTAGTTTTCTAACGCTATTT
CGCGCAGAAGCCAATCACAGCGAAATCGTCGAAACATTCTTACGTCAAGTGCGTATGACTCTCGAGTCGCATCCCTTGTT
TGATCACACTTGTCTCGTGCTCGGTCCAACCCCCGCTCCACTGGCCAAACGTGCGGGCAAATACCGCTGGCAGCTTCTGC
TTCAAACTCAACATCGTTCCTTGATGCAGAAAATACTCTCGAGCACGAAACCTGCTATCGATTTGTTACCAGATGCGAAA
AAAGTACGTTGGAATTTGGATATTGAGCCACAGGATCTGAGTTAG

Upstream 100 bases:

>100_bases
TACAGTTTTTCTCTCTCTGTTCGTGCATGCCCTTTTCTCGTATTTCTACCTCGCTTCCATTAGACTGTAAGTCATTATTT
TTGCCATGAAACACCACGTT

Downstream 100 bases:

>100_bases
CAAACGTTTACCACCTCACTCTAACGTTGAAAATTGCAACAATTTGCTTCTTTACGTGATGAACATCACGAATGAAATGC
GGATTTTGTTAATTCGCCCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLTGIVTALVQESDFPREQLKPIKAVLDHRPIWPDSVYS
LLLWCSHFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPQPHQAFLDE
EVASTTLRTLEEKGWISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQLGIIIV
DEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYL
ESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLF
RAEANHSEIVETFLRQVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
KVRWNLDIEPQDLS

Sequences:

>Translated_734_residues
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLTGIVTALVQESDFPREQLKPIKAVLDHRPIWPDSVYS
LLLWCSHFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPQPHQAFLDE
EVASTTLRTLEEKGWISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQLGIIIV
DEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYL
ESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLF
RAEANHSEIVETFLRQVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
KVRWNLDIEPQDLS
>Mature_734_residues
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLTGIVTALVQESDFPREQLKPIKAVLDHRPIWPDSVYS
LLLWCSHFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPQPHQAFLDE
EVASTTLRTLEEKGWISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQLGIIIV
DEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYL
ESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLF
RAEANHSEIVETFLRQVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
KVRWNLDIEPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=728, Percent_Identity=53.8461538461538, Blast_Score=780, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82018; Mature: 82018

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLTGIVTALVQESDFPREQ
CCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCHHH
LKPIKAVLDHRPIWPDSVYSLLLWCSHFYQYPLGETLHNALPAALRKGKAADFATLQEWQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
LTELGKNQLMQGLNSRAIKQAKVLNMLANGPQPHQAFLDEEVASTTLRTLEEKGWISCEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECC
KTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
CCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHH
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGI
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCEE
VIGTRSALLIPFAQLGIIIVDEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATP
EEECCCHHHEEHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCH
ALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYLESGLSAPLIAEMRRHLKAGN
HHHHHHHHHCCCEEEEEEHHHCCEECCCCCCEEEECCEEECCCCCCHHHHHHHHHHCCCC
QVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
EEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHEEHHHHCCCEEEEECCCCCCHHHHHCCC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGT
CCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEH
QMLAKGHHFPDVTLVALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTH
HHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
HPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLFRAEANHSEIVETFLRQVRMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
LESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
HHCCCCCCCEEEEECCCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
KVRWNLDIEPQDLS
CEEEECCCCCCCCC
>Mature Secondary Structure
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLTGIVTALVQESDFPREQ
CCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCHHH
LKPIKAVLDHRPIWPDSVYSLLLWCSHFYQYPLGETLHNALPAALRKGKAADFATLQEWQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
LTELGKNQLMQGLNSRAIKQAKVLNMLANGPQPHQAFLDEEVASTTLRTLEEKGWISCEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECC
KTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
CCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHH
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGI
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCEE
VIGTRSALLIPFAQLGIIIVDEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATP
EEECCCHHHEEHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCH
ALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYLESGLSAPLIAEMRRHLKAGN
HHHHHHHHHCCCEEEEEEHHHCCEECCCCCCEEEECCEEECCCCCCHHHHHHHHHHCCCC
QVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
EEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHEEHHHHCCCEEEEECCCCCCHHHHHCCC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGT
CCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEH
QMLAKGHHFPDVTLVALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTH
HHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
HPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLFRAEANHSEIVETFLRQVRMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
LESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
HHCCCCCCCEEEEECCCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
KVRWNLDIEPQDLS
CEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]