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The map label for this gene is fre [H]

Identifier: 27364375

GI number: 27364375

Start: 939697

End: 940410

Strand: Reverse

Name: fre [H]

Synonym: VV1_0933

Alternate gene names: 27364375

Gene position: 940410-939697 (Counterclockwise)

Preceding gene: 27364376

Following gene: 326423782

Centisome position: 28.65

GC content: 43.84

Gene sequence:

>714_bases
ATGACAATTCAATGTAAAGTAAAGTCGATTAAGCCACTAGCCAGCAACACTTACCAGATCCTATTGCATCCTGAAAAACC
AGTCTCCTTTAAAGCAGGTCAATATCTCATGGTGGTGATGGGTGAGAAAGACAAGCGTCCATTTTCCATTGCCAGTAGCC
CTTGTCGTCACGAAGGGGAGTTAGAGTTACATATCGGTGCTGCCGAACATAATGCTTATGCACTTGAAGTGGTGGAGGTG
ATGCAGAATGCATTGGATAACGATAGCTATATTGATATCGATGCACCACATGGCGATGCGTGGATCCGCGAAGAGAGCGA
AAGACCGATGTTACTCATTGCTGGTGGAACGGGTTTTAGTTATGTACGTTCTATTCTCGATCACTGTATCGCGCAGAATA
AGCCAAACTCAATTTATCTTTACTGGGGCGCTAGAGATTATTCACAACTTTACGCGAGCGAAGAGTTGGCTTTGATTGCC
AGCCAAAATGCGAATGTGCATTTTATTTCCGTCGTGGAAGAGTCTCCTGCGAATTGGCAGGGCAAAGTGGGTAACGTATT
ACAAGCCATCCACGAGGATTTCAGTAGTTTGGAAAACTACGATATTTACATCGCAGGTCGATTTGAGATGGCTGGCTTAG
CAAGAGAGCAATTTACACAGCAAAAACAAGCTAAGCGTGAAAGAATGTTTGCTGATGCATACGCCTTTATTTAA

Upstream 100 bases:

>100_bases
CTGAACAAGAACAACGACAAGGCTGGATATTTCCTTGTCTGGCCTATACAGAAAGTAATTTAGAACTTACCTTCGCCGAA
GAGTAAGTCAGAGGAATACC

Downstream 100 bases:

>100_bases
GTTTGTCCTATCATTTACCCCATCTTATTAGTCGCGAAAGGGGGTGTTTTGCTCTCTTTTGCGCCACATTGTTGGCTTTG
TAATCAAACAAACGCTTTTG

Product: FMN reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTIQCKVKSIKPLASNTYQILLHPEKPVSFKAGQYLMVVMGEKDKRPFSIASSPCRHEGELELHIGAAEHNAYALEVVEV
MQNALDNDSYIDIDAPHGDAWIREESERPMLLIAGGTGFSYVRSILDHCIAQNKPNSIYLYWGARDYSQLYASEELALIA
SQNANVHFISVVEESPANWQGKVGNVLQAIHEDFSSLENYDIYIAGRFEMAGLAREQFTQQKQAKRERMFADAYAFI

Sequences:

>Translated_237_residues
MTIQCKVKSIKPLASNTYQILLHPEKPVSFKAGQYLMVVMGEKDKRPFSIASSPCRHEGELELHIGAAEHNAYALEVVEV
MQNALDNDSYIDIDAPHGDAWIREESERPMLLIAGGTGFSYVRSILDHCIAQNKPNSIYLYWGARDYSQLYASEELALIA
SQNANVHFISVVEESPANWQGKVGNVLQAIHEDFSSLENYDIYIAGRFEMAGLAREQFTQQKQAKRERMFADAYAFI
>Mature_236_residues
TIQCKVKSIKPLASNTYQILLHPEKPVSFKAGQYLMVVMGEKDKRPFSIASSPCRHEGELELHIGAAEHNAYALEVVEVM
QNALDNDSYIDIDAPHGDAWIREESERPMLLIAGGTGFSYVRSILDHCIAQNKPNSIYLYWGARDYSQLYASEELALIAS
QNANVHFISVVEESPANWQGKVGNVLQAIHEDFSSLENYDIYIAGRFEMAGLAREQFTQQKQAKRERMFADAYAFI

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction. Preferably uses riboflavin as an electron acceptor when NADPH is used as an electron donor [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=236, Percent_Identity=47.4576271186441, Blast_Score=236, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 26723; Mature: 26592

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQCKVKSIKPLASNTYQILLHPEKPVSFKAGQYLMVVMGEKDKRPFSIASSPCRHEGE
CEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCE
LELHIGAAEHNAYALEVVEVMQNALDNDSYIDIDAPHGDAWIREESERPMLLIAGGTGFS
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCEEEEECCCCHH
YVRSILDHCIAQNKPNSIYLYWGARDYSQLYASEELALIASQNANVHFISVVEESPANWQ
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCC
GKVGNVLQAIHEDFSSLENYDIYIAGRFEMAGLAREQFTQQKQAKRERMFADAYAFI
CHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIQCKVKSIKPLASNTYQILLHPEKPVSFKAGQYLMVVMGEKDKRPFSIASSPCRHEGE
EEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCE
LELHIGAAEHNAYALEVVEVMQNALDNDSYIDIDAPHGDAWIREESERPMLLIAGGTGFS
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCEEEEECCCCHH
YVRSILDHCIAQNKPNSIYLYWGARDYSQLYASEELALIASQNANVHFISVVEESPANWQ
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCC
GKVGNVLQAIHEDFSSLENYDIYIAGRFEMAGLAREQFTQQKQAKRERMFADAYAFI
CHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8167139; 8206831; 6981058 [H]