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The map label for this gene is bioH

Identifier: 27364310

GI number: 27364310

Start: 872164

End: 872931

Strand: Reverse

Name: bioH

Synonym: VV1_0862

Alternate gene names: 27364310

Gene position: 872931-872164 (Counterclockwise)

Preceding gene: 27364315

Following gene: 27364309

Centisome position: 26.6

GC content: 54.95

Gene sequence:

>768_bases
ATGAGCACAGATTTACACTGGCAAACACAAGGTCAGGGGCCCGATTTGGTGTTGTTGCATGGCTGGGGCATGAATGGCGC
AGTGTGGCAGCAGGTGGTTGAAAGGCTGGAGCTGCACTTTCGTTTGCATGTGGTGGATCTGCCTGGCTATGGTCACAGTG
CCCATCTTCATGCGGCAAGTTTAGAAGAGATTGCCCAGCAGCTTCTCGAGCATGCACCGAAACAAGCCATTTGGGTTGGC
TGGTCATTAGGTGGTCTGGTGGCGACGCACATGGCGCTGCATCACGCCGATTACGTCAGCAAGTTAGTGACGGTGGCGAG
TTCACCCAAGTTCGCTGCAGAAGCGCGTTGGCGTGGCATTCAGCCGCAAGTATTGAGCGCCTTTACTGAGCAGTTGAGTG
AAGATTTTCACACCACGGTAGAGCGTTTTATGGCGCTGCAAGCCATGGGCAGCCCCTCTGCGCGACAAGATGTAAAAAAC
CTCAAACAAGCGGTGTTCTCGCGCCCGCAACCCAACCCCCAATCACTTTTGGCTGGGCTGCAAATGCTGGCGGAGGTGGA
CTTGCGTGACCATCTGCCGCACTTAACGATGCCGATGTTGCGTCTGTATGGCCGTTTGGACGGGTTGGTGCCGATCAAGG
TGGCGCAGGATCTCGAGAAAGTGTTACCCGCCAGTGAGCAGTTCATTTTCACCCAGTCGTCACACGCGCCTTTTATCACT
GAACCAGAGAGCTTTTGCCACCAGTTGCTGAGCTTTGCCGGCAAGTAA

Upstream 100 bases:

>100_bases
ATCGGTTAACATACGGGCAGTGGGGCGAATGAATCGTGTCGCTAGCGTACTCGACCACACGTGGCGCTTGAGGCTGGAAA
AAGTAGGAGTAAGAGGGAAG

Downstream 100 bases:

>100_bases
GCGAAAAATAAAATTAAACCTGTTCAAATATTGGTCGATACAAGAAGTAACCAAGGCAACTTACTACGGTTAGTGAAGCG
GTTTTGGGGCTGTGCAGTGA

Product: putative pimeloyl-BioC--CoA transferase BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLELHFRLHVVDLPGYGHSAHLHAASLEEIAQQLLEHAPKQAIWVG
WSLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLSEDFHTTVERFMALQAMGSPSARQDVKN
LKQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIKVAQDLEKVLPASEQFIFTQSSHAPFIT
EPESFCHQLLSFAGK

Sequences:

>Translated_255_residues
MSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLELHFRLHVVDLPGYGHSAHLHAASLEEIAQQLLEHAPKQAIWVG
WSLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLSEDFHTTVERFMALQAMGSPSARQDVKN
LKQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIKVAQDLEKVLPASEQFIFTQSSHAPFIT
EPESFCHQLLSFAGK
>Mature_254_residues
STDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLELHFRLHVVDLPGYGHSAHLHAASLEEIAQQLLEHAPKQAIWVGW
SLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLSEDFHTTVERFMALQAMGSPSARQDVKNL
KQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIKVAQDLEKVLPASEQFIFTQSSHAPFITE
PESFCHQLLSFAGK

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=251, Percent_Identity=53.7848605577689, Blast_Score=275, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_VIBVU (Q8DDU4)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759838.1
- ProteinModelPortal:   Q8DDU4
- GeneID:   1177801
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0862
- HOGENOM:   HBG748003
- OMA:   CTIASSP
- ProtClustDB:   CLSK874891
- BioCyc:   VVUL216895:VV1_0862-MONOMER
- BRENDA:   3.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 28418; Mature: 28287

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: ACT_SITE 82-82 ACT_SITE 207-207 ACT_SITE 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLELHFRLHVVDLPGYGHSAHLHAAS
CCCCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHH
LEEIAQQLLEHAPKQAIWVGWSLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
QPQVLSAFTEQLSEDFHTTVERFMALQAMGSPSARQDVKNLKQAVFSRPQPNPQSLLAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIKVAQDLEKVLPASEQFIFTQSSHAPFIT
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
EPESFCHQLLSFAGK
CHHHHHHHHHHHCCC
>Mature Secondary Structure 
STDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLELHFRLHVVDLPGYGHSAHLHAAS
CCCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHH
LEEIAQQLLEHAPKQAIWVGWSLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
QPQVLSAFTEQLSEDFHTTVERFMALQAMGSPSARQDVKNLKQAVFSRPQPNPQSLLAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIKVAQDLEKVLPASEQFIFTQSSHAPFIT
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
EPESFCHQLLSFAGK
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA