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The map label for this gene is cysN

Identifier: 27364179

GI number: 27364179

Start: 741936

End: 743366

Strand: Reverse

Name: cysN

Synonym: VV1_0725

Alternate gene names: 27364179

Gene position: 743366-741936 (Counterclockwise)

Preceding gene: 27364180

Following gene: 27364178

Centisome position: 22.65

GC content: 48.08

Gene sequence:

>1431_bases
ATGAATAGTGCAGTTCAAGCTCAGCTCGCTGAGTTAGGTATTGAAGGTTACCTAAATCAACACCAACACAAATCGCTACT
TAGATTCCTGACATGTGGCTCGGTAGACGATGGTAAAAGTACCCTGATTGGTCGTTTGCTACACGACTCAAAACAGATTT
ATGAAGATCAATTGGCCGCCGTACATAACGATAGCCAGCGCGTTGGCACCACCGGCAGCCGTCCAGACTTGGCGTTGTTA
GTGGATGGTCTGCAAGCGGAGCGTGAGCAAGGCATCACCATCGATGTGGCTTATCGCTACTTCTCAACCCAAAAGCGGAA
ATTCATTATCGCCGATACTCCAGGGCATGAGCAGTACACGCGCAACATGGCAACAGGTGCTTCTACCTGTGATCTTGCGG
TGATTTTGATCGATGCGCGTAAAGGGGTGTTGGATCAAACGCGTCGCCACTCGTTTATCTCCAATTTGCTCGGCCTGAAA
CACTTTGTGGTGGCGGTTAACAAAATGGACTTGGTGGAATTTTCTCAACAGCGTTTTGAAGAAATTAAAGCCGAATACCA
AGCGTTTTCGAAAAATCTTCGTGGTGAAACGGATATTCAAATTATCCCAATCTCTGCTCTGGAAGGCGACAACGTGGTTG
AACTAAGCCAGCAAATGGCTTGGTACCAAGGCCCGACACTACTGGAAATTTTGGAATCGGTGGACGTAGTAAAAGAGAAA
GAAGCGGGTGAATTCCGTTTCCCAGTGCAATACGTTAATCGTCCAAACCTCGATTTTCGTGGTTTCGCTGGCACCATTTC
GTCTGGTGTGGTGAAGGTGGGCGATCGCATTACGGCGTTGCCATCGGGTAAGAGCTCAACCGTTGCGCGCATCGTCACCT
TCGATGGCGATCTTGAACAGGCGCAAGCGGGTTTAGCAGTGACGCTGACTCTGGCCGATGAGATCGATATCAGCCGCGGG
GATTTGATTGTTCACCATGGTGCGAATGTGGAGCTCACTAACCATTTGGCAGCGGATGTGGTGTGGATGACAGAACAGCC
ACTTCAGCCTGGTCGTGATTATGACATCAAGATTGCTGGTAAAAAGACCATTGGTCGCGTTGAGCACATTCATCATCAAT
ATGACATCAACAACCTTTCTAAACACAGTGCGGCTGAGTTGCCATTGAATGGTATTGGTTTGTGTGAGTGGACGTTTAAT
GAGTCTATCGCTCTGGATAACTACCAAGACTGTGCTGATACTGGTGGTTTCATCATCATCGATCGCCTGACAAACGTCAC
TGTTGGTGCGGGCATGGTGAGTGAGAGCTTGACGGAAGTAACCAAAGCAAGCAGTGATTTCTCCGCATTTGAGTTGGAAT
TGAATGCACTCATTCGCAAGCATTTCCCACATTGGGATGCGAAAGATTTGAGCGAACTGCTTAAGAAGTAA

Upstream 100 bases:

>100_bases
CTTCCAGTGAGCGTCAAGGACGAGCCATCGACCACGATCAATCGGGTTCGATGGAACTGAAAAAACGTCAGGGTTACTTC
TAAGGATCTAAGGAAAGAAA

Downstream 100 bases:

>100_bases
CACTGAGTCATCATACCGAAGTTTGTAGAGTCAGTCAGACGTTGTTGTAACTGGGGGAGTGAGGCTCCCCCGTCATGGGG
ATGTAGTATGTGGGAGCAAG

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNSAVQAQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAAVHNDSQRVGTTGSRPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFVVAVNKMDLVEFSQQRFEEIKAEYQAFSKNLRGETDIQIIPISALEGDNVVELSQQMAWYQGPTLLEILESVDVVKEK
EAGEFRFPVQYVNRPNLDFRGFAGTISSGVVKVGDRITALPSGKSSTVARIVTFDGDLEQAQAGLAVTLTLADEIDISRG
DLIVHHGANVELTNHLAADVVWMTEQPLQPGRDYDIKIAGKKTIGRVEHIHHQYDINNLSKHSAAELPLNGIGLCEWTFN
ESIALDNYQDCADTGGFIIIDRLTNVTVGAGMVSESLTEVTKASSDFSAFELELNALIRKHFPHWDAKDLSELLKK

Sequences:

>Translated_476_residues
MNSAVQAQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAAVHNDSQRVGTTGSRPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFVVAVNKMDLVEFSQQRFEEIKAEYQAFSKNLRGETDIQIIPISALEGDNVVELSQQMAWYQGPTLLEILESVDVVKEK
EAGEFRFPVQYVNRPNLDFRGFAGTISSGVVKVGDRITALPSGKSSTVARIVTFDGDLEQAQAGLAVTLTLADEIDISRG
DLIVHHGANVELTNHLAADVVWMTEQPLQPGRDYDIKIAGKKTIGRVEHIHHQYDINNLSKHSAAELPLNGIGLCEWTFN
ESIALDNYQDCADTGGFIIIDRLTNVTVGAGMVSESLTEVTKASSDFSAFELELNALIRKHFPHWDAKDLSELLKK
>Mature_476_residues
MNSAVQAQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAAVHNDSQRVGTTGSRPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFVVAVNKMDLVEFSQQRFEEIKAEYQAFSKNLRGETDIQIIPISALEGDNVVELSQQMAWYQGPTLLEILESVDVVKEK
EAGEFRFPVQYVNRPNLDFRGFAGTISSGVVKVGDRITALPSGKSSTVARIVTFDGDLEQAQAGLAVTLTLADEIDISRG
DLIVHHGANVELTNHLAADVVWMTEQPLQPGRDYDIKIAGKKTIGRVEHIHHQYDINNLSKHSAAELPLNGIGLCEWTFN
ESIALDNYQDCADTGGFIIIDRLTNVTVGAGMVSESLTEVTKASSDFSAFELELNALIRKHFPHWDAKDLSELLKK

Specific function: May be the GTPase, regulating ATP sulfurylase activity

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily

Homologues:

Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=30.3030303030303, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI5729864, Length=429, Percent_Identity=30.3030303030303, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI4503471, Length=325, Percent_Identity=30.7692307692308, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4503475, Length=325, Percent_Identity=29.5384615384615, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI194097354, Length=307, Percent_Identity=29.9674267100977, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194018520, Length=307, Percent_Identity=29.9674267100977, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI194018522, Length=307, Percent_Identity=29.9674267100977, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI46094014, Length=307, Percent_Identity=29.6416938110749, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI34147630, Length=318, Percent_Identity=28.9308176100629, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1789108, Length=471, Percent_Identity=67.0912951167728, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=311, Percent_Identity=25.08038585209, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789737, Length=311, Percent_Identity=25.08038585209, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI2367247, Length=292, Percent_Identity=27.0547945205479, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI115532065, Length=316, Percent_Identity=30.6962025316456, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=316, Percent_Identity=30.6962025316456, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17552884, Length=325, Percent_Identity=31.3846153846154, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=325, Percent_Identity=31.3846153846154, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI32566629, Length=310, Percent_Identity=31.6129032258064, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=318, Percent_Identity=29.874213836478, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25141371, Length=285, Percent_Identity=27.3684210526316, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17556456, Length=344, Percent_Identity=27.906976744186, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322937, Length=380, Percent_Identity=30.5263157894737, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6325337, Length=323, Percent_Identity=30.3405572755418, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=323, Percent_Identity=30.3405572755418, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=437, Percent_Identity=27.0022883295195, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=239, Percent_Identity=29.7071129707113, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=218, Percent_Identity=30.2752293577982, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45550900, Length=443, Percent_Identity=25.9593679458239, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652838, Length=305, Percent_Identity=30.8196721311475, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17137572, Length=305, Percent_Identity=30.8196721311475, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45553807, Length=275, Percent_Identity=32.3636363636364, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553816, Length=275, Percent_Identity=32.3636363636364, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24651721, Length=275, Percent_Identity=32.3636363636364, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17864154, Length=275, Percent_Identity=32.3636363636364, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137380, Length=330, Percent_Identity=28.4848484848485, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281363316, Length=325, Percent_Identity=27.6923076923077, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17864358, Length=325, Percent_Identity=27.6923076923077, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921738, Length=275, Percent_Identity=25.4545454545455, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSN_VIBVU (Q8DE73)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759707.1
- ProteinModelPortal:   Q8DE73
- SMR:   Q8DE73
- GeneID:   1177670
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0725
- HOGENOM:   HBG307581
- OMA:   SAQIYED
- ProtClustDB:   PRK05124
- BioCyc:   VVUL216895:VV1_0725-MONOMER
- BRENDA:   2.7.7.4
- HAMAP:   MF_00062
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR02034
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: =2.7.7.4

Molecular weight: Translated: 52687; Mature: 52687

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAVQAQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAA
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHNDSQRVGTTGSRPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLKHFVVAVNKMDLVEFSQQRFE
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EIKAEYQAFSKNLRGETDIQIIPISALEGDNVVELSQQMAWYQGPTLLEILESVDVVKEK
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
EAGEFRFPVQYVNRPNLDFRGFAGTISSGVVKVGDRITALPSGKSSTVARIVTFDGDLEQ
CCCCEECCHHHCCCCCCCCCCCCCHHHCCCEECCCEEEECCCCCCCCEEEEEEECCCHHH
AQAGLAVTLTLADEIDISRGDLIVHHGANVELTNHLAADVVWMTEQPLQPGRDYDIKIAG
HCCCEEEEEEEECCCCCCCCCEEEECCCCEEECCHHHHEEEEECCCCCCCCCCCEEEEEC
KKTIGRVEHIHHQYDINNLSKHSAAELPLNGIGLCEWTFNESIALDNYQDCADTGGFIII
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCHHHHCCCCEEEE
DRLTNVTVGAGMVSESLTEVTKASSDFSAFELELNALIRKHFPHWDAKDLSELLKK
EECCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNSAVQAQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAA
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHNDSQRVGTTGSRPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLKHFVVAVNKMDLVEFSQQRFE
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EIKAEYQAFSKNLRGETDIQIIPISALEGDNVVELSQQMAWYQGPTLLEILESVDVVKEK
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
EAGEFRFPVQYVNRPNLDFRGFAGTISSGVVKVGDRITALPSGKSSTVARIVTFDGDLEQ
CCCCEECCHHHCCCCCCCCCCCCCHHHCCCEECCCEEEECCCCCCCCEEEEEEECCCHHH
AQAGLAVTLTLADEIDISRGDLIVHHGANVELTNHLAADVVWMTEQPLQPGRDYDIKIAG
HCCCEEEEEEEECCCCCCCCCEEEECCCCEEECCHHHHEEEEECCCCCCCCCCCEEEEEC
KKTIGRVEHIHHQYDINNLSKHSAAELPLNGIGLCEWTFNESIALDNYQDCADTGGFIII
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCHHHHCCCCEEEE
DRLTNVTVGAGMVSESLTEVTKASSDFSAFELELNALIRKHFPHWDAKDLSELLKK
EECCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA