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The map label for this gene is pmbA [H]

Identifier: 27364155

GI number: 27364155

Start: 716257

End: 717600

Strand: Reverse

Name: pmbA [H]

Synonym: VV1_0699

Alternate gene names: 27364155

Gene position: 717600-716257 (Counterclockwise)

Preceding gene: 27364157

Following gene: 27364142

Centisome position: 21.87

GC content: 50.67

Gene sequence:

>1344_bases
ATGGATGTAAAACAGCAAGTTGCTCAGCAAAGAGTTGAGCTCGAAGCCGCGGTTGCCAAAGCGCTCGCGCTGGCAGCGCA
ACATAGCGATGGTGCTGAAGTTGCGATCAACAAAACCACGGGCTTAAGTGTTTCGACTCGCATGTGTGAAGTAGAAAACG
TCGAGTTTAACAGTGATGGCGCTTTAGGCATTACGGTTTATCGTGGTCAGCGTAAAGGCAGCGCGTCGACATCGGATCTC
AGTGAAAAAGCGATTGAACAGACTGTCTTGGCGGCCTTGGATATTGCGCAATACACGTCAGAAGATCCCTGTGCTGGGCC
TGCTCCAGCTGAGTTGATGGTGAAAGAGATTCCGGATTTGGATCTCTTCCATCCAGATACGCCCGATCCCGATTATGCGG
CGAAAATGGCCATTGCTGCTGAAGAGCATGCGCTTAACTACAGCGATAAAATTAAGCAGAGTGATGGCGCGAGTTACGAC
AGCCATTACGGCGTTCGCGTCTACGGCAACAGTCACGGCTTATTGGCAAGTTATGCCTCGAGTCGCCATAGCATCAGTTG
TTGTGTGATTGGTGAAGGCAAAAATGGCGAAATGGAGCGTGACTACAGCTACACTGTCGCCCGTCATCGTGATGACATGT
GGCGTCCTGAACAAGTCGGTCGCGAAGCGGCGCAAAAAACCATTGAACGTTTAGATGCCCGCAAGCTAAAAACGGGCAAA
TACCCGGTGATGTTTGCCGCGGATGTGGCAACCGGGCTTATCGGTCATTTAGTGATGGCGATCAGTGGCGGTAACTTGTA
TCGCAAATCGTCTTTCTTGCTTGATCATTTGGGCAAACAAGTGCTGCCAGAGTGGTTCAATATCTCTGAGCGCCCGCATA
TTCTGCGTGGTTTGGCATCAAGCCCATTCGACAGCGAAGGGGTTTACACGCAAGATCGTGAAATCATCACCGATGGTGTG
CTCGCCACTTATCTGCTCACCAGTTACGCTGCGCGTAAAATGAATATGGCGCCAACGGGGCATGCTGGTGGTATCCACAA
CTGGTTTGTGCAATCGACAGGGCAAGATTTCCAACAGATGCTCAAAGAGCTGGGTACGGGTTTACTTGTGACCGAAGTGA
TGGGTCAAGGTGTGAATGTGGTGACCGGCGATTATTCGCGTGGCGCGGCGGGTTTCTGGGTGGAAAATGGCGAGTTGCAA
TTCCCAGTGTCAGAAATCACCATTGCGGGTAATTTGAAAGAGATGTTCCAGCAAATCGTTGCCGTTGGTAGCGATGTAGA
GACGCGCTCACAAATTCAAACCGGTTCTATTTTGTTGCCATCGATGAAGATTGCGGGAGAATAA

Upstream 100 bases:

>100_bases
CTTTCTCAAAATAAGATTAGCCCTATTTTAACAAGAATCGTGGGGAGAAAGCGAAATTCTTGTTATTCTTATGCCATTAC
AAATCAAAGATGATTGAGAT

Downstream 100 bases:

>100_bases
CCGCGATTGCGGCATTCGTTAAGAGATGAAAAAAGGTTGCTCAGTAAGCAACCTTTTTTGTTTCGTTTCTATTCTGCTTT
CATTTTGATAGCGTCAGGTC

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDVKQQVAQQRVELEAAVAKALALAAQHSDGAEVAINKTTGLSVSTRMCEVENVEFNSDGALGITVYRGQRKGSASTSDL
SEKAIEQTVLAALDIAQYTSEDPCAGPAPAELMVKEIPDLDLFHPDTPDPDYAAKMAIAAEEHALNYSDKIKQSDGASYD
SHYGVRVYGNSHGLLASYASSRHSISCCVIGEGKNGEMERDYSYTVARHRDDMWRPEQVGREAAQKTIERLDARKLKTGK
YPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKQVLPEWFNISERPHILRGLASSPFDSEGVYTQDREIITDGV
LATYLLTSYAARKMNMAPTGHAGGIHNWFVQSTGQDFQQMLKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGELQ
FPVSEITIAGNLKEMFQQIVAVGSDVETRSQIQTGSILLPSMKIAGE

Sequences:

>Translated_447_residues
MDVKQQVAQQRVELEAAVAKALALAAQHSDGAEVAINKTTGLSVSTRMCEVENVEFNSDGALGITVYRGQRKGSASTSDL
SEKAIEQTVLAALDIAQYTSEDPCAGPAPAELMVKEIPDLDLFHPDTPDPDYAAKMAIAAEEHALNYSDKIKQSDGASYD
SHYGVRVYGNSHGLLASYASSRHSISCCVIGEGKNGEMERDYSYTVARHRDDMWRPEQVGREAAQKTIERLDARKLKTGK
YPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKQVLPEWFNISERPHILRGLASSPFDSEGVYTQDREIITDGV
LATYLLTSYAARKMNMAPTGHAGGIHNWFVQSTGQDFQQMLKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGELQ
FPVSEITIAGNLKEMFQQIVAVGSDVETRSQIQTGSILLPSMKIAGE
>Mature_447_residues
MDVKQQVAQQRVELEAAVAKALALAAQHSDGAEVAINKTTGLSVSTRMCEVENVEFNSDGALGITVYRGQRKGSASTSDL
SEKAIEQTVLAALDIAQYTSEDPCAGPAPAELMVKEIPDLDLFHPDTPDPDYAAKMAIAAEEHALNYSDKIKQSDGASYD
SHYGVRVYGNSHGLLASYASSRHSISCCVIGEGKNGEMERDYSYTVARHRDDMWRPEQVGREAAQKTIERLDARKLKTGK
YPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKQVLPEWFNISERPHILRGLASSPFDSEGVYTQDREIITDGV
LATYLLTSYAARKMNMAPTGHAGGIHNWFVQSTGQDFQQMLKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGELQ
FPVSEITIAGNLKEMFQQIVAVGSDVETRSQIQTGSILLPSMKIAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=64.2058165548098, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1789640, Length=418, Percent_Identity=23.2057416267943, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48502; Mature: 48502

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVKQQVAQQRVELEAAVAKALALAAQHSDGAEVAINKTTGLSVSTRMCEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHEEEEEECCEECCCC
ALGITVYRGQRKGSASTSDLSEKAIEQTVLAALDIAQYTSEDPCAGPAPAELMVKEIPDL
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
DLFHPDTPDPDYAAKMAIAAEEHALNYSDKIKQSDGASYDSHYGVRVYGNSHGLLASYAS
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHC
SRHSISCCVIGEGKNGEMERDYSYTVARHRDDMWRPEQVGREAAQKTIERLDARKLKTGK
CCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
YPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKQVLPEWFNISERPHILRGLAS
CCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCC
SPFDSEGVYTQDREIITDGVLATYLLTSYAARKMNMAPTGHAGGIHNWFVQSTGQDFQQM
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHH
LKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGELQFPVSEITIAGNLKEMFQQIV
HHHHCCCEEEHHHHCCCCEEEECCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHH
AVGSDVETRSQIQTGSILLPSMKIAGE
HCCCCCHHHHHHCCCCEEECCHHCCCC
>Mature Secondary Structure
MDVKQQVAQQRVELEAAVAKALALAAQHSDGAEVAINKTTGLSVSTRMCEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHEEEEEECCEECCCC
ALGITVYRGQRKGSASTSDLSEKAIEQTVLAALDIAQYTSEDPCAGPAPAELMVKEIPDL
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
DLFHPDTPDPDYAAKMAIAAEEHALNYSDKIKQSDGASYDSHYGVRVYGNSHGLLASYAS
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHC
SRHSISCCVIGEGKNGEMERDYSYTVARHRDDMWRPEQVGREAAQKTIERLDARKLKTGK
CCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
YPVMFAADVATGLIGHLVMAISGGNLYRKSSFLLDHLGKQVLPEWFNISERPHILRGLAS
CCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCC
SPFDSEGVYTQDREIITDGVLATYLLTSYAARKMNMAPTGHAGGIHNWFVQSTGQDFQQM
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHH
LKELGTGLLVTEVMGQGVNVVTGDYSRGAAGFWVENGELQFPVSEITIAGNLKEMFQQIV
HHHHCCCEEEHHHHCCCCEEEECCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHH
AVGSDVETRSQIQTGSILLPSMKIAGE
HCCCCCHHHHHHCCCCEEECCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]