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The map label for this gene is murC

Identifier: 27364038

GI number: 27364038

Start: 590497

End: 591957

Strand: Reverse

Name: murC

Synonym: VV1_0577

Alternate gene names: 27364038

Gene position: 591957-590497 (Counterclockwise)

Preceding gene: 326423733

Following gene: 326423732

Centisome position: 18.04

GC content: 49.08

Gene sequence:

>1461_bases
ATGACTATTCAACACAAGCAAGATCTTGCACAGATTCGCGCTATGGTTCCAGAGATGCGCCGAGTGAAATCCATCCACTT
CATTGGTATTGGTGGCGCAGGTATGAGCGGCATTGCCGAAGTGTTGCTCAATGAAGGGTATCAAATCACCGGCTCTGATA
TTGCGGAAAATGCCGTTACTGAGCGCTTGGCTCAAAAAGGCGCGAAAGTGTATATCGGCCACCAAGCGACGAATGTTGCA
GAGGCGAGTGTTGTTGTTGTCTCAACAGCGATCAATGAAGCGAACCCAGAAGTTAAAGCGGCGCGTGAAGCTCGCATTCC
GGTTGTTCGCCGTGCAGAAATGCTGGCGGAACTGATGCGTTTTCGTCATGGTATCGCCGTCGCTGGTACGCATGGTAAAA
CCACCACGACAGCATTGGTGACGCAAATTTATTCAGAAGCGGGACTGGATCCAACGTTCGTGAATGGTGGTCTGGTAAAA
AGTGCAGGTACCAATGCGCGTTTAGGTTCTAGCCGCATTTTGATTGCTGAAGCCGATGAAAGTGATGCGTCGTTCTTACA
TTTGCAACCGATGGTGTGTATTGTCACAAACATTGAAGCTGACCATATGGATACCTATGGCGGTGACTTCGAAACACTGA
AGCAAACCTTCATCGACTTCTTACACAATTTGCCATTTTATGGTCAGGCGATTGTTTGTATTGATGATCCGGTGATTCGC
GAGCTGATCCCGCGCATCAGTCGTCAAGTCATTACTTACGGCTTCTCGGAAGATGCCGATGTTCGCATTGAAAATTACCG
TCAAGAAGGCCAGCAAGGAAAATTCACCGTGGTCCGCAAAGATTGCGAAGCGCTGGATATTACCCTGAATATTCCTGGCC
GCCACAATGCATTGAATGCCGCTGCGGCCATTGCTGTTGCAACGGAAGATGAAATTGGCGATGACGCGATCCTTGCTGCC
ATGGTTGGTACCCAAGGTACTGGTCGCCGCTTCGAGCATCTGGGTGAGTTTGAAACAGGCAACGGAAACGTAATGCTGGT
TGATGACTATGGTCATCACCCAACCGAAGTGGATGTGACCATCCAAGCTGCACGAAACGGTTGGCAAGAGAAGCGTCTCG
TGATGATTTTCCAACCCCACCGTTACAGCCGTACCCGAGATCTCTACGATGATTTCGCGAACGTGCTTGAGCAGGTTGAT
GTGTTGGTCATGTTAGATGTGTACGCGGCGGGTGAAAAAGCGATTGCTGGAGCGGACAGTCGTTCATTGTGCCGCACGAT
TCGCAGTCGTGGAAAAATCGACCCTATTTTTGTTGCAGATACCCAACAACTGCCAGAGGTATTGGCGAACATTTTACAAG
AAGGCGATCTGTTATTAGCACAAGGTGCGGGGGATATTGGTAAAGTCGCCCGACAATTGGCAGCACTTGAGCTAAATATC
AGTAATATGAAAGCAAAATAG

Upstream 100 bases:

>100_bases
ATGGCGCTTCACGCCCGAGCTGCAGCACAAAATAATGCCGACCAAGTTGTGGCGGATGCGATTGTCGCCCTAACTGAACA
GAAATAAAGAGAACAGGTTA

Downstream 100 bases:

>100_bases
CCATTTGTGGCGGATATTAAGCAGTTAGTAACGCAGATCTCGTATTTGCGATAACTTCGTTGCGATTTCTCTCTATCAAT
GTTTGATAGTTTACAAGAGC

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: UDP-N-acetylmuramoyl-L-alanine synthetase

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTIQHKQDLAQIRAMVPEMRRVKSIHFIGIGGAGMSGIAEVLLNEGYQITGSDIAENAVTERLAQKGAKVYIGHQATNVA
EASVVVVSTAINEANPEVKAAREARIPVVRRAEMLAELMRFRHGIAVAGTHGKTTTTALVTQIYSEAGLDPTFVNGGLVK
SAGTNARLGSSRILIAEADESDASFLHLQPMVCIVTNIEADHMDTYGGDFETLKQTFIDFLHNLPFYGQAIVCIDDPVIR
ELIPRISRQVITYGFSEDADVRIENYRQEGQQGKFTVVRKDCEALDITLNIPGRHNALNAAAAIAVATEDEIGDDAILAA
MVGTQGTGRRFEHLGEFETGNGNVMLVDDYGHHPTEVDVTIQAARNGWQEKRLVMIFQPHRYSRTRDLYDDFANVLEQVD
VLVMLDVYAAGEKAIAGADSRSLCRTIRSRGKIDPIFVADTQQLPEVLANILQEGDLLLAQGAGDIGKVARQLAALELNI
SNMKAK

Sequences:

>Translated_486_residues
MTIQHKQDLAQIRAMVPEMRRVKSIHFIGIGGAGMSGIAEVLLNEGYQITGSDIAENAVTERLAQKGAKVYIGHQATNVA
EASVVVVSTAINEANPEVKAAREARIPVVRRAEMLAELMRFRHGIAVAGTHGKTTTTALVTQIYSEAGLDPTFVNGGLVK
SAGTNARLGSSRILIAEADESDASFLHLQPMVCIVTNIEADHMDTYGGDFETLKQTFIDFLHNLPFYGQAIVCIDDPVIR
ELIPRISRQVITYGFSEDADVRIENYRQEGQQGKFTVVRKDCEALDITLNIPGRHNALNAAAAIAVATEDEIGDDAILAA
MVGTQGTGRRFEHLGEFETGNGNVMLVDDYGHHPTEVDVTIQAARNGWQEKRLVMIFQPHRYSRTRDLYDDFANVLEQVD
VLVMLDVYAAGEKAIAGADSRSLCRTIRSRGKIDPIFVADTQQLPEVLANILQEGDLLLAQGAGDIGKVARQLAALELNI
SNMKAK
>Mature_485_residues
TIQHKQDLAQIRAMVPEMRRVKSIHFIGIGGAGMSGIAEVLLNEGYQITGSDIAENAVTERLAQKGAKVYIGHQATNVAE
ASVVVVSTAINEANPEVKAAREARIPVVRRAEMLAELMRFRHGIAVAGTHGKTTTTALVTQIYSEAGLDPTFVNGGLVKS
AGTNARLGSSRILIAEADESDASFLHLQPMVCIVTNIEADHMDTYGGDFETLKQTFIDFLHNLPFYGQAIVCIDDPVIRE
LIPRISRQVITYGFSEDADVRIENYRQEGQQGKFTVVRKDCEALDITLNIPGRHNALNAAAAIAVATEDEIGDDAILAAM
VGTQGTGRRFEHLGEFETGNGNVMLVDDYGHHPTEVDVTIQAARNGWQEKRLVMIFQPHRYSRTRDLYDDFANVLEQVDV
LVMLDVYAAGEKAIAGADSRSLCRTIRSRGKIDPIFVADTQQLPEVLANILQEGDLLLAQGAGDIGKVARQLAALELNIS
NMKAK

Specific function: Cell wall formation

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786279, Length=478, Percent_Identity=71.1297071129707, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1790680, Length=469, Percent_Identity=26.865671641791, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURC_VIBVU (Q8DEL1)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759566.1
- ProteinModelPortal:   Q8DEL1
- SMR:   Q8DEL1
- GeneID:   1177528
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0577
- HOGENOM:   HBG476594
- OMA:   YQPHRYS
- ProtClustDB:   PRK00421
- BioCyc:   VVUL216895:VV1_0577-MONOMER
- BRENDA:   6.3.2.8
- GO:   GO:0005737
- HAMAP:   MF_00046
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR016040
- InterPro:   IPR005758
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01082

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.8

Molecular weight: Translated: 52961; Mature: 52830

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQHKQDLAQIRAMVPEMRRVKSIHFIGIGGAGMSGIAEVLLNEGYQITGSDIAENAVT
CCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCEECCCHHHHHHHH
ERLAQKGAKVYIGHQATNVAEASVVVVSTAINEANPEVKAAREARIPVVRRAEMLAELMR
HHHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
FRHGIAVAGTHGKTTTTALVTQIYSEAGLDPTFVNGGLVKSAGTNARLGSSRILIAEADE
HHCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCEEECCCCCCCCCCCEEEEEECCC
SDASFLHLQPMVCIVTNIEADHMDTYGGDFETLKQTFIDFLHNLPFYGQAIVCIDDPVIR
CCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
ELIPRISRQVITYGFSEDADVRIENYRQEGQQGKFTVVRKDCEALDITLNIPGRHNALNA
HHHHHHHHHHEEECCCCCCCEEHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCHHH
AAAIAVATEDEIGDDAILAAMVGTQGTGRRFEHLGEFETGNGNVMLVDDYGHHPTEVDVT
HHEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCEECCCCCEEEEECCCCCCCEEEEE
IQAARNGWQEKRLVMIFQPHRYSRTRDLYDDFANVLEQVDVLVMLDVYAAGEKAIAGADS
EEEHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCCCCCH
RSLCRTIRSRGKIDPIFVADTQQLPEVLANILQEGDLLLAQGAGDIGKVARQLAALELNI
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHEEEH
SNMKAK
HCCCCC
>Mature Secondary Structure 
TIQHKQDLAQIRAMVPEMRRVKSIHFIGIGGAGMSGIAEVLLNEGYQITGSDIAENAVT
CCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCEECCCHHHHHHHH
ERLAQKGAKVYIGHQATNVAEASVVVVSTAINEANPEVKAAREARIPVVRRAEMLAELMR
HHHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
FRHGIAVAGTHGKTTTTALVTQIYSEAGLDPTFVNGGLVKSAGTNARLGSSRILIAEADE
HHCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCEEECCCCCCCCCCCEEEEEECCC
SDASFLHLQPMVCIVTNIEADHMDTYGGDFETLKQTFIDFLHNLPFYGQAIVCIDDPVIR
CCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
ELIPRISRQVITYGFSEDADVRIENYRQEGQQGKFTVVRKDCEALDITLNIPGRHNALNA
HHHHHHHHHHEEECCCCCCCEEHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCHHH
AAAIAVATEDEIGDDAILAAMVGTQGTGRRFEHLGEFETGNGNVMLVDDYGHHPTEVDVT
HHEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCEECCCCCEEEEECCCCCCCEEEEE
IQAARNGWQEKRLVMIFQPHRYSRTRDLYDDFANVLEQVDVLVMLDVYAAGEKAIAGADS
EEEHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCCCCCH
RSLCRTIRSRGKIDPIFVADTQQLPEVLANILQEGDLLLAQGAGDIGKVARQLAALELNI
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHEEEH
SNMKAK
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA