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The map label for this gene is nfo

Identifier: 27364003

GI number: 27364003

Start: 541509

End: 542390

Strand: Reverse

Name: nfo

Synonym: VV1_0541

Alternate gene names: 27364003

Gene position: 542390-541509 (Counterclockwise)

Preceding gene: 27364005

Following gene: 27364002

Centisome position: 16.53

GC content: 45.92

Gene sequence:

>882_bases
ATGACAAACAGCAAAAACCGTTTTGGTAACAAATTTATTGGCGCGCATGTTTCAGCGGCGGGCGGGGTTGACAATGCACC
TCTGCGGGCGCGTGAGATTGGAGCAAACGCGTTTGCTTTGTTCACGAAGAATCAACGCCAATGGGTTGCCAAGCCATTAG
AAGAGAAAACCATCTCGGCGTTCAAAGCCAATTGCGCATTGTTGGGTTTTTCTCCACAGCAAATTCTTCCCCATGATTCT
TATCTTATTAATTTAGGTGCGCCAGAAGCGGAGAAGCTGGAAAAGTCTCGTCTCGCGTTTATTGATGAAATGGAGCGATG
CCAGCTGCTAGGGTTGAACTTACTCAATTTCCACCCAGGCAGCCATTTGGAGAAGATCTCAGAAAAAGCGTGTTTGTCTT
TAATCGCAGAATCGATCAACTTAGCGCATCAAGCGGTACCTGATGTGGTGGCGGTGATAGAAAACACGGCCGGACAAGGT
TCCAACTTAGGTTGGCGATTTGAGCACCTTGCAGAAATCATTGAGCAGGTTGAAGATAAGAGTCGAGTTGGGGTTTGTTT
GGATACTTGCCACACCTTTGCCGCAGGCTATGATCTGCGAACTCCTGAGGCGTGTGAGACGACCTTCGCTGAGTTTGAAC
GTGTGGTCGGAATGCACTATTTACGTGCAATGCATATTAATGATTCAAAAGTAAAATTAGCCAGTAAGGTGGATAGGCAC
CATGCCCTAGGGAAAGGTGAAATTGGCTGGGACTGTTTTGAATACATTGCGAAAGACAGCCGTTTTGATTCTATCCCATT
GATTTTAGAGACTATTGAACCTGAACTTTGGCCGCAAGAAATTGAGCAGTTGAGAAAATATCATCTCTCTTCAATCGCTT
AG

Upstream 100 bases:

>100_bases
AATTTTGTTGTAAATTTACTACAAAATTAATTGGAAATTTGACTGAACTAAGCTAAATGGTTGTAATTAGCCCCTTATCT
TGAAAGTGTTAAGTGATTAA

Downstream 100 bases:

>100_bases
CGCTTTTTGTCATCAGATTGGCATCTTTCTTTCATAGCAAACAAGAACAATAGTGTTTATCGAGTTTGTCACAGGAGGAT
GCCACTATGTTTACAACCAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISAFKANCALLGFSPQQILPHDS
YLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPGSHLEKISEKACLSLIAESINLAHQAVPDVVAVIENTAGQG
SNLGWRFEHLAEIIEQVEDKSRVGVCLDTCHTFAAGYDLRTPEACETTFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA

Sequences:

>Translated_293_residues
MTNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISAFKANCALLGFSPQQILPHDS
YLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPGSHLEKISEKACLSLIAESINLAHQAVPDVVAVIENTAGQG
SNLGWRFEHLAEIIEQVEDKSRVGVCLDTCHTFAAGYDLRTPEACETTFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA
>Mature_292_residues
TNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISAFKANCALLGFSPQQILPHDSY
LINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPGSHLEKISEKACLSLIAESINLAHQAVPDVVAVIENTAGQGS
NLGWRFEHLAEIIEQVEDKSRVGVCLDTCHTFAAGYDLRTPEACETTFAEFERVVGMHYLRAMHINDSKVKLASKVDRHH
ALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=275, Percent_Identity=67.6363636363636, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17531193, Length=272, Percent_Identity=48.1617647058824, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6322735, Length=260, Percent_Identity=44.6153846153846, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_VIBVU (Q8DEP6)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759531.1
- ProteinModelPortal:   Q8DEP6
- SMR:   Q8DEP6
- GeneID:   1177493
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0541
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   VVUL216895:VV1_0541-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 32680; Mature: 32548

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISA
CCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHH
FKANCALLGFSPQQILPHDSYLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPG
HHCCEEEECCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCC
SHLEKISEKACLSLIAESINLAHQAVPDVVAVIENTAGQGSNLGWRFEHLAEIIEQVEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SRVGVCLDTCHTFAAGYDLRTPEACETTFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA
HCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISA
CCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHH
FKANCALLGFSPQQILPHDSYLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPG
HHCCEEEECCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCC
SHLEKISEKACLSLIAESINLAHQAVPDVVAVIENTAGQGSNLGWRFEHLAEIIEQVEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SRVGVCLDTCHTFAAGYDLRTPEACETTFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA
HCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA