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The map label for this gene is aroF [H]

Identifier: 27363959

GI number: 27363959

Start: 492663

End: 493733

Strand: Reverse

Name: aroF [H]

Synonym: VV1_0495

Alternate gene names: 27363959

Gene position: 493733-492663 (Counterclockwise)

Preceding gene: 27363960

Following gene: 27363958

Centisome position: 15.04

GC content: 47.71

Gene sequence:

>1071_bases
ATGCAGAAAAGTGAACTAAGCAATATTAATATCAGCGATGAACAAGTGCTGATTACGCCAGAACAGCTAAAACAGAAACT
GCCACTGAGCGACAACGCCCGCCGCTTCATTCAAGAGTCGCGTAATACCGTCGCCAATATTATTCATAAGCAAGATCATC
GTCTGCTGGTGGTTTGTGGTCCTTGTTCTATTCACGATCTGGACGCGGCAAAGGATTACGCGCGTCGCCTAAAAGCGCTC
TCCGCCGAGTTAAGCGATCAACTGTATATTGTGATGCGTGTTTACTTTGAAAAACCTCGTACTACTGTGGGTTGGAAAGG
GCTCATCAACGACCCTCATCTGGATGGTAGCTTCGATATTGAGCATGGTCTGCATGTTGGCCGTCAATTGCTGGTGGAGC
TGGCTGAAATGGAGATCCCTCTGGCGACAGAAGCACTCGACCCAATTAGCCCGCAATACTTGGCTGATACATTCAGTTGG
GCTGCGATTGGTGCGCGTACGACAGAATCACAAACTCACCGTGAAATGGCCAGTGGTTTGTCTATGCCGATTGGTTTTAA
AAATGGCACGGATGGCAGCTTGTCAACGGCGATCAACGCAATGCAAGCGGCCTCTTCAAGCCACCGTTTTATGGGGATCA
ACCGTGAAGGCCAAGTGGCATTGCTGACGACTCAAGGTAACCCAAATGGTCACGTGATTTTGCGTGGTGGTAAGCAGACC
AACTACGATTCTGTGTCGGTGGCGGAGTGTGAGCAAGACATGGCCAAAGTGGGTTTAGATGCGTCATTGATGGTGGATTG
TAGCCATGCCAATTCACGCAAAGATTACCGTCGTCAGCCTTTAGTCGCCGAAGACGTGATTCATCAAATTCGCGAAGGCA
ACAAATCGATCATTGGTTTGATGATTGAAAGCCACATTAATGAAGGTAATCAATCCTCAGATTTACCGCTTTCTGAGATG
CGGTACGGTGTTTCTATTACCGATGCCTGTATCAATTGGCAGGCAACTGAGGCACTATTGCGTAAAGCACACAAAGAGTT
AGTGCCACATTTAGAAAACCGTCTCAAATAA

Upstream 100 bases:

>100_bases
TTACGGTTGATTTTTAATTCAACCCTGTTAACTTGTGTTCAAACAGATTTCGACTCTCAGGGATATGAGGGTGTAAAAAA
TAATATACAGGTTGAGTGTC

Downstream 100 bases:

>100_bases
GGATAGAGCAAGGTAAATCATGGCAGTTGAACTGAACGCATTGCGTGAACAGATTGATGCAGTAGACAAACAGATGTTGG
AGCTATTGGCTCAGCGCTTG

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQKSELSNINISDEQVLITPEQLKQKLPLSDNARRFIQESRNTVANIIHKQDHRLLVVCGPCSIHDLDAAKDYARRLKAL
SAELSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDIEHGLHVGRQLLVELAEMEIPLATEALDPISPQYLADTFSW
AAIGARTTESQTHREMASGLSMPIGFKNGTDGSLSTAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
NYDSVSVAECEQDMAKVGLDASLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNKSIIGLMIESHINEGNQSSDLPLSEM
RYGVSITDACINWQATEALLRKAHKELVPHLENRLK

Sequences:

>Translated_356_residues
MQKSELSNINISDEQVLITPEQLKQKLPLSDNARRFIQESRNTVANIIHKQDHRLLVVCGPCSIHDLDAAKDYARRLKAL
SAELSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDIEHGLHVGRQLLVELAEMEIPLATEALDPISPQYLADTFSW
AAIGARTTESQTHREMASGLSMPIGFKNGTDGSLSTAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
NYDSVSVAECEQDMAKVGLDASLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNKSIIGLMIESHINEGNQSSDLPLSEM
RYGVSITDACINWQATEALLRKAHKELVPHLENRLK
>Mature_356_residues
MQKSELSNINISDEQVLITPEQLKQKLPLSDNARRFIQESRNTVANIIHKQDHRLLVVCGPCSIHDLDAAKDYARRLKAL
SAELSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDIEHGLHVGRQLLVELAEMEIPLATEALDPISPQYLADTFSW
AAIGARTTESQTHREMASGLSMPIGFKNGTDGSLSTAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
NYDSVSVAECEQDMAKVGLDASLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNKSIIGLMIESHINEGNQSSDLPLSEM
RYGVSITDACINWQATEALLRKAHKELVPHLENRLK

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=355, Percent_Identity=69.5774647887324, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1786969, Length=337, Percent_Identity=54.5994065281899, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787996, Length=328, Percent_Identity=48.780487804878, Blast_Score=335, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319726, Length=333, Percent_Identity=54.6546546546547, Blast_Score=357, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6320240, Length=347, Percent_Identity=48.9913544668588, Blast_Score=346, Evalue=4e-96,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 39524; Mature: 39524

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSELSNINISDEQVLITPEQLKQKLPLSDNARRFIQESRNTVANIIHKQDHRLLVVCG
CCCCCCCCCCCCCCEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PCSIHDLDAAKDYARRLKALSAELSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDI
CCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCH
EHGLHVGRQLLVELAEMEIPLATEALDPISPQYLADTFSWAAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SMPIGFKNGTDGSLSTAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCEEEEECCCCC
NYDSVSVAECEQDMAKVGLDASLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNKSIIGL
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEH
MIESHINEGNQSSDLPLSEMRYGVSITDACINWQATEALLRKAHKELVPHLENRLK
HHHHHCCCCCCCCCCCHHHHHCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKSELSNINISDEQVLITPEQLKQKLPLSDNARRFIQESRNTVANIIHKQDHRLLVVCG
CCCCCCCCCCCCCCEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PCSIHDLDAAKDYARRLKALSAELSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDI
CCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCH
EHGLHVGRQLLVELAEMEIPLATEALDPISPQYLADTFSWAAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SMPIGFKNGTDGSLSTAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCEEEEECCCCC
NYDSVSVAECEQDMAKVGLDASLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNKSIIGL
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEH
MIESHINEGNQSSDLPLSEMRYGVSITDACINWQATEALLRKAHKELVPHLENRLK
HHHHHCCCCCCCCCCCHHHHHCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]