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The map label for this gene is flgH

Identifier: 27363702

GI number: 27363702

Start: 213734

End: 214513

Strand: Reverse

Name: flgH

Synonym: VV1_0220

Alternate gene names: 27363702

Gene position: 214513-213734 (Counterclockwise)

Preceding gene: 27363703

Following gene: 161501852

Centisome position: 6.54

GC content: 49.1

Gene sequence:

>780_bases
ATGAAACGCATCAGTCTGATTGCTTTAGTCACCATCATGAGTGGCTGTACCATGCTTGAGCCAATCGAAACCCCAGAAGT
GGTCAATGCGACGACAGTGGTGGATGCGGTTGAAGGGGATAAGTCGAAGGACGAGAGCTCGGGTATCGTCGATACCTTGC
GTGGACGTAGCGATCCCGTGGCAGGCGATCCTGCGTGGGCTCCCATTCATCCGAAACAGCAGCCAGAGCACTACGCTGCG
GAAACGGGCTCGCTATTTAGCGTGAATCATTTGTCGAATCTTTATGATGACTCGAAGCCTCGCGGCGTCGGTGACATCAT
TACCGTCACTCTGGATGAAAAAACCAATGCTTCCAAAAGTGCCAATGCGGACCTGTCGAAAAGCAACGATTCAAGCATGG
ATCCCCTTGAAGTGGGTGGGCAAGAGCTCAAAATCGACGGTAAGTATAATTTCTCTTACAACCTGACCAACTCAAACAAC
TTCACTGGCGATGCGTCGGCAAAACAGAGCAACAGCATCAGCGGTTACATCACCGTGGAAGTGATCGAAGTACTGGCCAA
TGGCAACCTCGTGATTCGTGGTGAGAAGTGGCTGACGCTGAATACGGGTGATGAGTACATTCGCCTCAGTGGCACAATTC
GTCCTGATGATATTAGTTTTGACAACACTATTGCGTCAAATCGCGTTTCTAACGCAAGAATTCAATATTCTGGTACCGGG
ACACAACAAGATATGCAAGAGCCTGGATTCTTGGCACGATTCTTTAATGTCTCTCTATAA

Upstream 100 bases:

>100_bases
ATGAATTCAAAAGTCATCTCATCGGTCGATAAGATGATGAGTTTTGTTAACCAGCAGCTTTAAGCGCTGGTTAGCTAAAC
GAGTAGGAGAGAATATCGCC

Downstream 100 bases:

>100_bases
GCGGCAAAGCCGATGGCAATTTGACGGCTTGATCCTGCACTAGAGAAAGGTAAGTTAAGATGAAAAAGTTCACCCTACTG
TTGTTATGTTTTGTATTGCC

Product: flagellar basal body L-ring protein

Products: NA

Alternate protein names: Basal body L-ring protein

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKRISLIALVTIMSGCTMLEPIETPEVVNATTVVDAVEGDKSKDESSGIVDTLRGRSDPVAGDPAWAPIHPKQQPEHYAA
ETGSLFSVNHLSNLYDDSKPRGVGDIITVTLDEKTNASKSANADLSKSNDSSMDPLEVGGQELKIDGKYNFSYNLTNSNN
FTGDASAKQSNSISGYITVEVIEVLANGNLVIRGEKWLTLNTGDEYIRLSGTIRPDDISFDNTIASNRVSNARIQYSGTG
TQQDMQEPGFLARFFNVSL

Sequences:

>Translated_259_residues
MKRISLIALVTIMSGCTMLEPIETPEVVNATTVVDAVEGDKSKDESSGIVDTLRGRSDPVAGDPAWAPIHPKQQPEHYAA
ETGSLFSVNHLSNLYDDSKPRGVGDIITVTLDEKTNASKSANADLSKSNDSSMDPLEVGGQELKIDGKYNFSYNLTNSNN
FTGDASAKQSNSISGYITVEVIEVLANGNLVIRGEKWLTLNTGDEYIRLSGTIRPDDISFDNTIASNRVSNARIQYSGTG
TQQDMQEPGFLARFFNVSL
>Mature_259_residues
MKRISLIALVTIMSGCTMLEPIETPEVVNATTVVDAVEGDKSKDESSGIVDTLRGRSDPVAGDPAWAPIHPKQQPEHYAA
ETGSLFSVNHLSNLYDDSKPRGVGDIITVTLDEKTNASKSANADLSKSNDSSMDPLEVGGQELKIDGKYNFSYNLTNSNN
FTGDASAKQSNSISGYITVEVIEVLANGNLVIRGEKWLTLNTGDEYIRLSGTIRPDDISFDNTIASNRVSNARIQYSGTG
TQQDMQEPGFLARFFNVSL

Specific function: Assembles around the rod to form the L-ring and probably protects the motor/basal body from shearing forces during rotation

COG id: COG2063

COG function: function code N; Flagellar basal body L-ring protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable). Bacterial flagellum basal body

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flgH family

Homologues:

Organism=Escherichia coli, GI1787319, Length=204, Percent_Identity=35.7843137254902, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLGH_VIBVU (Q8DFJ1)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759230.1
- GeneID:   1177172
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0220
- HOGENOM:   HBG507455
- OMA:   IAGEKQI
- ProtClustDB:   PRK00249
- BioCyc:   VVUL216895:VV1_0220-MONOMER
- HAMAP:   MF_00415
- InterPro:   IPR000527
- PRINTS:   PR01008

Pfam domain/function: PF02107 FlgH

EC number: NA

Molecular weight: Translated: 28013; Mature: 28013

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRISLIALVTIMSGCTMLEPIETPEVVNATTVVDAVEGDKSKDESSGIVDTLRGRSDPV
CCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHEEEECCCCCCCCCCCCHHHHHCCCCCCC
AGDPAWAPIHPKQQPEHYAAETGSLFSVNHLSNLYDDSKPRGVGDIITVTLDEKTNASKS
CCCCCCCCCCCCCCCHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCC
ANADLSKSNDSSMDPLEVGGQELKIDGKYNFSYNLTNSNNFTGDASAKQSNSISGYITVE
CCCCCCCCCCCCCCHHHCCCEEEEEECEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHE
VIEVLANGNLVIRGEKWLTLNTGDEYIRLSGTIRPDDISFDNTIASNRVSNARIQYSGTG
EEHHHHCCCEEEECCEEEEECCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCEEEECCCC
TQQDMQEPGFLARFFNVSL
CHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MKRISLIALVTIMSGCTMLEPIETPEVVNATTVVDAVEGDKSKDESSGIVDTLRGRSDPV
CCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHEEEECCCCCCCCCCCCHHHHHCCCCCCC
AGDPAWAPIHPKQQPEHYAAETGSLFSVNHLSNLYDDSKPRGVGDIITVTLDEKTNASKS
CCCCCCCCCCCCCCCHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCC
ANADLSKSNDSSMDPLEVGGQELKIDGKYNFSYNLTNSNNFTGDASAKQSNSISGYITVE
CCCCCCCCCCCCCCHHHCCCEEEEEECEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHE
VIEVLANGNLVIRGEKWLTLNTGDEYIRLSGTIRPDDISFDNTIASNRVSNARIQYSGTG
EEHHHHCCCEEEECCEEEEECCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCEEEECCCC
TQQDMQEPGFLARFFNVSL
CHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA