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The map label for this gene is ligA

Identifier: 27363688

GI number: 27363688

Start: 195546

End: 197555

Strand: Reverse

Name: ligA

Synonym: VV1_0206

Alternate gene names: 27363688

Gene position: 197555-195546 (Counterclockwise)

Preceding gene: 27363689

Following gene: 27363687

Centisome position: 6.02

GC content: 48.56

Gene sequence:

>2010_bases
ATGTCTGAATCCAAGCAGCAATATTTAGAAGAATTAAAACAGCAACTGCACTATCACGCAGTTCGTTACTACGTTGAAGA
TAATCCTGAAATTCCGGATGCCGAGTATGACCGTATGATGCGCGAGTTAATGGCCATTGAAGCGGAGCATCCGGAGTGGA
TTAGTGTTGACTCACCCAGTCAGCGAGTGGGAGGTGTCGCTCTTGATAGTTTCCGCCAAGTAACGCATGAGATCCCGATG
CTTTCTTTGGACAACGCCTTTAGCGATGAAGAGCTTGAGAGTTTCCTTAAACGCGCACAAGATCGTATGCCAAGTGCTCA
TATTGATGCATTTTGTTGCGAGCCTAAGCTCGATGGTTTAGCTGTCAGCCTTCTCTATGAAAATGGCGTTTTGGTTCAGG
CCGCGACTCGAGGCGATGGCACCACAGGCGAAAATATTACCGAAAACGTGCGTACCATTGCTTCTGTGCCACTGAAATTG
CAGGGAGAAGGTTGGCCATCACGCATCGAAGTTCGTGGTGAAGTCTTCATGCCAAAAGCAGGCTTTGAAAAACTCAACGA
CATCGCTCGCAAGAAAGGCGAAAAAGTATTCGTTAATCCGCGTAATGCAGCGGCGGGAAGCTTACGCCAGCTTGATTCGA
AGATCACCGCAACTCGTCCGTTGGCGTTTTATGCGTATAGCGTGGGCGTCGTAGAGGGCATCTCACTGTCATCAAGCCAT
TACCAGCGTTTCTTGCAGTTAAAGCAATGGGGCTTGCCTATGTGCCCTGAGACGAAGTTGGTTAATGGGTTGGAAAGCGT
TAAGGCATTCTACGCAGACATCTTAAATCGACGCGATGCATTGCCATATGAAATCGATGGTGTCGTGATTAAGATTGACG
ACATTGTGATTCAAGAGAAATTGGGTTTTGTGGCACGCGCACCGCGTTGGGCTATCGCGTATAAGTTTCCTGCGCAAGAA
GAACTCACCCTGTTGAATGACGTTGAGTTTCAAGTTGGCAGAACGGGGGCTATTACCCCAGTCGCGAAGCTGGAACCTGT
TTTCGTCGGTGGTGTTACCGTCAGTAATGCAACGCTTCATAACGCTGATGAAATCGAACGCCTTGGTGTGATGGTTGGTG
ATACCGTCGTGATTCGACGCGCCGGGGATGTGATTCCACAAATTGTTTCTGTGGTGAAAGATCGTCGTAAAGGAGATGAA
AAAAACATCATTTTCCCGACCGCTTGCCCCGTTTGTCACTCACATGTTGAACGGATTGAGGGAGAAGCGGTGACTCGCTG
CTCTGGCGGTTTGGTGTGTCAAGCACAGCGAAAAGAGGCGCTGAAACATTTTGTGTCTAGGAAGGCGCTCGATGTCGATG
GGTTGGGTGACAAGGTCATAGAGCAGCTTGTAGACAAAGAAATGGTAGAGACTCCAGCGGACTTGTTTACGTTATCTGCA
GGCGTTTTAACGGTACTTGAACGAATGGGACCAAAGTCAGCCCAGAACATCGTCAACGCACTGAACGTTGCGAAAGAGAC
TACGTTGGCTCGTTTTCTTTATTCTCTGGGGATCAGAGAAGTGGGCGAGGCAACCGCGGCTAATTTGGCTCGCCATTTTA
AGACACTAGAAGCGATACAAACTGCAACGCATGAGCAGTTAATCGAAGTTCCCGATATCGGTGAAGTCGTCGCTCGTCAT
ATCACGGCATTTTTTGCTGAAGAGAAAAATCAAAGAGTGGTGCAAGCACTTATTGAGCAGGGAATTGTGTGGCCCGCTGT
CGAGGAGCTTGGTAGTGAGATACCTCAGCCATTAGCAGGCAAAGTTGTGGTACTAACCGGAACCTTGACGCAACTCAGCC
GTGGCGATGCAAAAGCGGCTTTGGAACGATTGGGGGCTAAAGTCACGGGCAGTGTATCCAAGAAAACAGATATCGTTTTT
GCTGGTGAAGCGGCGGGTTCAAAATTAACCAAAGCGCAAGAACTTGGCGTGGAAATTCAAACAGAGCAAGATTTACTCGC
ACTTCTGTAA

Upstream 100 bases:

>100_bases
AGGCAAATAGTTGAGTTCAAAGCGGCAAACGCTTAAGCTTCCAATATCCCAACAGGGCTCCCAGCGGAGCCCTTTTTTAT
CGTGATTTAAGGGCTGGAAA

Downstream 100 bases:

>100_bases
CCTAAAAAAGATAAAATAAAAACTAAAACCCTCTAAATTGACGTTTAGAGGGTTATTTTTTTATGTGAAATAAATACTGG
TTAACGAATGAAAATAACGA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPSQRVGGVALDSFRQVTHEIPM
LSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGLAVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKL
QGEGWPSRIEVRGEVFMPKAGFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEKLGFVARAPRWAIAYKFPAQE
ELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKDRRKGDE
KNIIFPTACPVCHSHVERIEGEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQTATHEQLIEVPDIGEVVARH
ITAFFAEEKNQRVVQALIEQGIVWPAVEELGSEIPQPLAGKVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVF
AGEAAGSKLTKAQELGVEIQTEQDLLALL

Sequences:

>Translated_669_residues
MSESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPSQRVGGVALDSFRQVTHEIPM
LSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGLAVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKL
QGEGWPSRIEVRGEVFMPKAGFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEKLGFVARAPRWAIAYKFPAQE
ELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKDRRKGDE
KNIIFPTACPVCHSHVERIEGEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQTATHEQLIEVPDIGEVVARH
ITAFFAEEKNQRVVQALIEQGIVWPAVEELGSEIPQPLAGKVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVF
AGEAAGSKLTKAQELGVEIQTEQDLLALL
>Mature_668_residues
SESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPSQRVGGVALDSFRQVTHEIPML
SLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGLAVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKLQ
GEGWPSRIEVRGEVFMPKAGFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSHY
QRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEKLGFVARAPRWAIAYKFPAQEE
LTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKDRRKGDEK
NIIFPTACPVCHSHVERIEGEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSAG
VLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQTATHEQLIEVPDIGEVVARHI
TAFFAEEKNQRVVQALIEQGIVWPAVEELGSEIPQPLAGKVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVFA
GEAAGSKLTKAQELGVEIQTEQDLLALL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=64.9700598802395, Blast_Score=890, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=561, Percent_Identity=22.4598930481283, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_VIBVU (Q8DFK5)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759216.1
- HSSP:   O87703
- ProteinModelPortal:   Q8DFK5
- GeneID:   1177158
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0206
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   VVUL216895:VV1_0206-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73536; Mature: 73405

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 116-116 BINDING 114-114 BINDING 137-137 BINDING 174-174 BINDING 291-291 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPS
CCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCH
QRVGGVALDSFRQVTHEIPMLSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGL
HHHCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCE
AVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKLQGEGWPSRIEVRGEVFMPKA
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECEEECCCC
GFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
CHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEHHHHHHHH
LGFVARAPRWAIAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLH
HHHHHCCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCHHHCCCEEECCEEECCCCCC
NADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKDRRKGDEKNIIFPTACPVCHSHVERIE
CHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHC
GEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
CCHHEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
TATHEQLIEVPDIGEVVARHITAFFAEEKNQRVVQALIEQGIVWPAVEELGSEIPQPLAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
KVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVFAGEAAGSKLTKAQELGVEIQ
EEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCEEE
TEQDLLALL
CCHHHHHCC
>Mature Secondary Structure 
SESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPS
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCH
QRVGGVALDSFRQVTHEIPMLSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGL
HHHCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCE
AVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKLQGEGWPSRIEVRGEVFMPKA
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECEEECCCC
GFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
CHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEHHHHHHHH
LGFVARAPRWAIAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLH
HHHHHCCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCHHHCCCEEECCEEECCCCCC
NADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKDRRKGDEKNIIFPTACPVCHSHVERIE
CHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHC
GEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
CCHHEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
TATHEQLIEVPDIGEVVARHITAFFAEEKNQRVVQALIEQGIVWPAVEELGSEIPQPLAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
KVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVFAGEAAGSKLTKAQELGVEIQ
EEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCEEE
TEQDLLALL
CCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA