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The map label for this gene is yfcE [H]

Identifier: 27363676

GI number: 27363676

Start: 185573

End: 186130

Strand: Reverse

Name: yfcE [H]

Synonym: VV1_0194

Alternate gene names: 27363676

Gene position: 186130-185573 (Counterclockwise)

Preceding gene: 326423667

Following gene: 27363671

Centisome position: 5.67

GC content: 46.59

Gene sequence:

>558_bases
GTGCTTGTGAAATTATTTTTTGCTTCGGATTTGCATGGCAGCCTAGAGGCGACAGAGCAAACATTGGCGTTATTTGAACA
ATCAGGAGCGGATCACCTCATTTTACTGGGGGACATACTCAATCATGGACCAAGGAACCCTGTTCCTTCGTCTTATAATC
CACCTCAAGTGGCTGAGAAATTAAATCAGTACGCCAACCAAATTATCGCGGTCAGAGGCAATTGCGATAGTGAAGTGGAT
CAGATGTTGCTCTCTTTTCCAATGATGATGGATTACAGTTGGGTATTGCTTGAATCAGGCGCTCGACTCTTTCTCACTCA
TGGTCATCTGTACAATGCCAAACAACAACCTGCGTTGAAAGCCGGGGATATTTTGGCGCATGGGCATAGCCATGTAGCGC
TAGCGGAGCCGCAAGGGGAGATCGTCGTTTTTAATCCGGGCTCTGTGACTTTTCCTCGGGAAAATCAACTGGCTAGCTAT
GGCCTCTTTGAAAATGGCACCTTGTCCGTCATTTCATTACAAGGTGAGCTGTTACACTCCTGCCAAATTGGTGGCTAA

Upstream 100 bases:

>100_bases
ATTGGTATGGTCAGCTACAACAATATTGTGATCAATGCCATGGCTCGGGATGACGAGTAGTCTTTGCAGCGTTAGAATAC
CCTAGTATTTTTAGGGGGAA

Downstream 100 bases:

>100_bases
AATCCAGCACGTCAAATAAGGCAGTCACCACTGCCTTATTTTTTGTCAACGTGACCAAGATCCCTTTCGGGGGCGATGAT
ATCGCGGACTCTTTGTTTGA

Product: phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MLVKLFFASDLHGSLEATEQTLALFEQSGADHLILLGDILNHGPRNPVPSSYNPPQVAEKLNQYANQIIAVRGNCDSEVD
QMLLSFPMMMDYSWVLLESGARLFLTHGHLYNAKQQPALKAGDILAHGHSHVALAEPQGEIVVFNPGSVTFPRENQLASY
GLFENGTLSVISLQGELLHSCQIGG

Sequences:

>Translated_185_residues
MLVKLFFASDLHGSLEATEQTLALFEQSGADHLILLGDILNHGPRNPVPSSYNPPQVAEKLNQYANQIIAVRGNCDSEVD
QMLLSFPMMMDYSWVLLESGARLFLTHGHLYNAKQQPALKAGDILAHGHSHVALAEPQGEIVVFNPGSVTFPRENQLASY
GLFENGTLSVISLQGELLHSCQIGG
>Mature_185_residues
MLVKLFFASDLHGSLEATEQTLALFEQSGADHLILLGDILNHGPRNPVPSSYNPPQVAEKLNQYANQIIAVRGNCDSEVD
QMLLSFPMMMDYSWVLLESGARLFLTHGHLYNAKQQPALKAGDILAHGHSHVALAEPQGEIVVFNPGSVTFPRENQLASY
GLFENGTLSVISLQGELLHSCQIGG

Specific function: Shows phosphodiesterase activity [H]

COG id: COG0622

COG function: function code R; Predicted phosphoesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily. YfcE family [H]

Homologues:

Organism=Escherichia coli, GI1788638, Length=175, Percent_Identity=57.7142857142857, Blast_Score=209, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR020935
- InterPro:   IPR000979 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 20149; Mature: 20149

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVKLFFASDLHGSLEATEQTLALFEQSGADHLILLGDILNHGPRNPVPSSYNPPQVAEK
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCCCCHHHHHH
LNQYANQIIAVRGNCDSEVDQMLLSFPMMMDYSWVLLESGARLFLTHGHLYNAKQQPALK
HHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCEECCCCCCCCC
AGDILAHGHSHVALAEPQGEIVVFNPGSVTFPRENQLASYGLFENGTLSVISLQGELLHS
CCCEEECCCCEEEEECCCCCEEEECCCCEECCCCCCCEECCCCCCCCEEEEEECCHHHHH
CQIGG
CCCCC
>Mature Secondary Structure
MLVKLFFASDLHGSLEATEQTLALFEQSGADHLILLGDILNHGPRNPVPSSYNPPQVAEK
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCCCCHHHHHH
LNQYANQIIAVRGNCDSEVDQMLLSFPMMMDYSWVLLESGARLFLTHGHLYNAKQQPALK
HHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCEECCCCCCCCC
AGDILAHGHSHVALAEPQGEIVVFNPGSVTFPRENQLASYGLFENGTLSVISLQGELLHS
CCCEEECCCCEEEEECCCCCEEEECCCCEECCCCCCCEECCCCCCCCEEEEEECCHHHHH
CQIGG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]