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The map label for this gene is rfaH [H]

Identifier: 27363666

GI number: 27363666

Start: 174366

End: 174872

Strand: Reverse

Name: rfaH [H]

Synonym: VV1_0184

Alternate gene names: 27363666

Gene position: 174872-174366 (Counterclockwise)

Preceding gene: 27363668

Following gene: 326423665

Centisome position: 5.33

GC content: 45.36

Gene sequence:

>507_bases
ATGAAAAAATGGTATTTGCTGTATTGTAAGCGCGGAGAGCAGATCCGAGCGAAGCAACATTTAGAAAACCAAGGTGTTGA
GTGTTTCTATCCCACCATAGAAGTTGAAAAGATTCTGCGTGGTAAACGCCAAACAAAGAGTGAGCCCTTATTTCCTTCCT
ACATTTTTGTTCGTTTCGATTTTGAAATAGGCCCGACGTTTACCACTGTGCGTTCAACCAGAGGTGTGGTGGATTTTGTG
AAGTTTGGCGCTCACCCCAAAGAGCTTCAAGGGGATTTGATTTATGAACTCAAGCTTCTTGAACAAGAGGGACATGAAAG
TGCGTTGCAGGGGAGTAATTTGCCGAAACCGGGTGATTCGATCAAAGTGAAAAGCGGGCAGTTCGCGGGTATCGAGGCCA
TTTTCCAAGAGCAAGATGGCGAAACACGTTCAATCATGCTGGTTAAAATGATTTCCCAGACCGTGCCAGTGAGCATTGAG
AATAGCGACCTAGAACTGAAGGGGTGA

Upstream 100 bases:

>100_bases
TAGCAAACAAAGTGTATTGGTTTGTATGATTGCAACTCACGCGATTTCCTATCGTCTGGCGAAATGCTAGGATAAACAAA
AAATAGATGGCTAGTGATAA

Downstream 100 bases:

>100_bases
GTGGCCAATAGACCCTAGTAAAGAAGGCACCTTTTGGGTGCCTTCTTTCGTTGCTGTTAGTGTGAACGGCGTGCAGATTA
ATACGCTTCGTTATGTACCG

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MKKWYLLYCKRGEQIRAKQHLENQGVECFYPTIEVEKILRGKRQTKSEPLFPSYIFVRFDFEIGPTFTTVRSTRGVVDFV
KFGAHPKELQGDLIYELKLLEQEGHESALQGSNLPKPGDSIKVKSGQFAGIEAIFQEQDGETRSIMLVKMISQTVPVSIE
NSDLELKG

Sequences:

>Translated_168_residues
MKKWYLLYCKRGEQIRAKQHLENQGVECFYPTIEVEKILRGKRQTKSEPLFPSYIFVRFDFEIGPTFTTVRSTRGVVDFV
KFGAHPKELQGDLIYELKLLEQEGHESALQGSNLPKPGDSIKVKSGQFAGIEAIFQEQDGETRSIMLVKMISQTVPVSIE
NSDLELKG
>Mature_168_residues
MKKWYLLYCKRGEQIRAKQHLENQGVECFYPTIEVEKILRGKRQTKSEPLFPSYIFVRFDFEIGPTFTTVRSTRGVVDFV
KFGAHPKELQGDLIYELKLLEQEGHESALQGSNLPKPGDSIKVKSGQFAGIEAIFQEQDGETRSIMLVKMISQTVPVSIE
NSDLELKG

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=164, Percent_Identity=45.1219512195122, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 19156; Mature: 19156

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWYLLYCKRGEQIRAKQHLENQGVECFYPTIEVEKILRGKRQTKSEPLFPSYIFVRFD
CCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCHHHHHHCCHHCCCCCCCCCEEEEEEEE
FEIGPTFTTVRSTRGVVDFVKFGAHPKELQGDLIYELKLLEQEGHESALQGSNLPKPGDS
EEECCCEEEHHHCCCHHHHHHHCCCHHHHCCCEEEEEEEEHHCCCHHHHCCCCCCCCCCC
IKVKSGQFAGIEAIFQEQDGETRSIMLVKMISQTVPVSIENSDLELKG
EEEECCCCCCHHHEEECCCCCHHHHHHEEHHHHHCCEEECCCCEEECC
>Mature Secondary Structure
MKKWYLLYCKRGEQIRAKQHLENQGVECFYPTIEVEKILRGKRQTKSEPLFPSYIFVRFD
CCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCHHHHHHCCHHCCCCCCCCCEEEEEEEE
FEIGPTFTTVRSTRGVVDFVKFGAHPKELQGDLIYELKLLEQEGHESALQGSNLPKPGDS
EEECCCEEEHHHCCCHHHHHHHCCCHHHHCCCEEEEEEEEHHCCCHHHHCCCCCCCCCCC
IKVKSGQFAGIEAIFQEQDGETRSIMLVKMISQTVPVSIENSDLELKG
EEEECCCCCCHHHEEECCCCCHHHHHHEEHHHHHCCEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]