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The map label for this gene is truB [H]

Identifier: 270000605

GI number: 270000605

Start: 3623142

End: 3624065

Strand: Reverse

Name: truB [H]

Synonym: BA_3947

Alternate gene names: 270000605

Gene position: 3624065-3623142 (Counterclockwise)

Preceding gene: 30263814

Following gene: 30263813

Centisome position: 69.33

GC content: 35.82

Gene sequence:

>924_bases
ATGGAAGGTGTAGTATTATTACATAAGCCAAAAGGCATGACATCACATGATTGTGTATTTAAATTAAGAAAGATATTGCG
TGAAAAACGAATTGGTCATACAGGAACATTAGATCCAGATGTAACAGGAGTATTGCCGATTTGTGTTGGGCGAGCAACAA
AGATTGCGCAATTTTTAACGAGTGAGACGAAAACATATGAAGGTGAAGTGACATTAGGGTTTTCAACAACAACTGAAGAT
GCTTCTGGTGAAGTTGTGGAAACGAAATATGTAGATCGTGTTATTACTCGTAAAGAAGTGGAAGAGGCTCTTGCGACATT
AACAGGTACGATTGAGCAAATGCCTCCGATGTTTTCGGCTGTAAAAGTTAACGGAAAAAAATTATATGAATATGCAAGAG
CAGGACAAGAGGTTGAACGTCCAGTTCGTACAATTACAATTCATGAATTTGTATTACTAGATGACCGTGAAGTGTTTGAA
GGAGAAAATATTTCATTCCGTTTCCGTGTAACGTGTAGTAAAGGAACATATGTAAGAACATTAGCGGTAATGATTGGTGA
AAAACTTGGTTTTCCATCACATATGTCTCACCTTGTAAGAACGGCTTCTGGTGAATTTTTATTAGAGGATTGCATATCAT
TTGAAGAAATTGAGGAAAACGTGCAAAATGGAACAGTAGAGTCTATCTTCATTTCAATTGATGAAGCATTAAGTAAATTC
CCAAAAATGGTTGTAGATGAAAAGCAAGCAGAAAAAATAAAGAATGGTATGTTCTTAAAAAATGAATTGCAAATAACGGC
GCCATTTATAACAGTGTTTGATAAAAACGATCGTTGCCTTGCAATTTATGAGCACCATCCAAAGCACCCTGGTATGCTAA
AGCCGATGAAAGTACTTGTGAATAATCAAGAACTAAAGCTATAA

Upstream 100 bases:

>100_bases
AACGTGAAGAATAATGGATAGACATTTGTCTATCCATTTTTTCAATGTAAAATAAGTTTTGAATAAGTATTATGAAAGAA
TTACGATGAGGTGAGCATAT

Downstream 100 bases:

>100_bases
TGAATTATTGGAAGGATTATTACAGAAAAAGGTGAATTCAAGCGTGAAACTTATTCATTTAACTCATCCACATGAACAAA
ATAAATTAGAATTACCACCT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVETKYVDRVITRKEVEEALATLTGTIEQMPPMFSAVKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFE
GENISFRFRVTCSKGTYVRTLAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLVNNQELKL

Sequences:

>Translated_307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVETKYVDRVITRKEVEEALATLTGTIEQMPPMFSAVKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFE
GENISFRFRVTCSKGTYVRTLAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLVNNQELKL
>Mature_307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVETKYVDRVITRKEVEEALATLTGTIEQMPPMFSAVKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFE
GENISFRFRVTCSKGTYVRTLAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLVNNQELKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=258, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4503337, Length=202, Percent_Identity=34.1584158415842, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI215599015, Length=202, Percent_Identity=34.1584158415842, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI2367200, Length=265, Percent_Identity=38.1132075471698, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17553978, Length=202, Percent_Identity=30.6930693069307, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=265, Percent_Identity=29.0566037735849, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=140, Percent_Identity=40, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=32.6732673267327, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=32.6732673267327, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=32.6732673267327, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=32.6732673267327, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=32.6732673267327, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=32.6732673267327, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34644; Mature: 34644

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHH
SETKTYEGEVTLGFSTTTEDASGEVVETKYVDRVITRKEVEEALATLTGTIEQMPPMFSA
HCCCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFEGENISFRFRVTCSKGTYVRT
EEECHHHHHHHHHCCHHHHCCHHEEEEEEEEEECCHHHHCCCCEEEEEEEEECCCCHHHH
LAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHHEEEHHHHHHHH
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLV
HHHHHCHHHHHHHHCCCEEECCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHC
NNQELKL
CCCCCCC
>Mature Secondary Structure
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHH
SETKTYEGEVTLGFSTTTEDASGEVVETKYVDRVITRKEVEEALATLTGTIEQMPPMFSA
HCCCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFEGENISFRFRVTCSKGTYVRT
EEECHHHHHHHHHCCHHHHCCHHEEEEEEEEEECCHHHHCCCCEEEEEEEEECCCCHHHH
LAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHHEEEHHHHHHHH
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLV
HHHHHCHHHHHHHHCCCEEECCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHC
NNQELKL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA