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The map label for this gene is rng [H]

Identifier: 270000531

GI number: 270000531

Start: 4252120

End: 4253511

Strand: Reverse

Name: rng [H]

Synonym: BA_4677

Alternate gene names: 270000531

Gene position: 4253511-4252120 (Counterclockwise)

Preceding gene: 30264515

Following gene: 30264513

Centisome position: 81.37

GC content: 35.27

Gene sequence:

>1392_bases
TTGAAAACGTTATATATGAACTACGCTGGATCGGAAAAACGCGTTGCAATTGAAGAGAAGAAGAAAATTGTTGAGTTTTT
ATGGAAACGAAATGAAGAACAAGAGATTGTTGGACATATTTATATTGGACGTATTGTAAGAACGATTGCTGGAATGAACG
CAGCTTTTGTAAACATCGGTTTAGAAAAACATGCGTATCTTTCGTATGATGATGTACCGTCTGCTTATCGTATACATGAA
GGGCAAGCGGTACTCGTACAAGTTGTAAAAGAGGCAATTGATACGAAAGGGCCGAAATTGACAGCAAATATAGAATTTAC
CGGGAAATATGTCGTTTATATGCCGTATGATGAAATGCGTGCGGTTTCTCGGAAAATAAAAAACAATAAAAGAAGGCAAC
AACTACTCCAAATTGAAGTAGAAGGGACAGGAGGTTATATTTTCCGCTCTGCTTCTGAAAAAGGAGAAATTGAAGAAATA
CAAGCTGAAATGCAAAGGCTACAGCAGTTATATGAAGAGTTAAAAAGAAAAGAGGAACAAGGAAAGGCACCGTTACTACT
TCATCGACCGGCAACTTTTTTAGATCGTGTATTTCAAGAAAATCCAATTGAAACGATTGAAAAAGTAGTTGTAGATACAA
GGAGTATAGTAAAAGAATTAGAAGAAAAGGTCGGTAAAGAGAAAGTATCTTTTTATAACGAAAAATCTTCAATGTTTAAC
CATTTTGGAATAGAGCGTGAAATTGAGAAAGCACTTCAAAAAATTGTGTGGCTTCCAAATGGTGCTTATTTAATTGTGGA
ACAAATGGAGACGATGACTGTAATTGATGTGAATACGGGCAAGTTTACTGGAAAACAGAATTTACAAGATACAGTTCTTC
GTACAAATGAATTGGCGGCTGAAGAGATAGCGCGTCAATTAAGACTGCGTGATATCGGTGGTATGATATTAATTGATTTT
ATTAATATGAAAAGAAGAGAAGATAAAGAAAAGGTAAGAGAATGTCTCGTGGCTGCTATGCAAAATGATCGTACATATAC
AAGGGTACTCGGGTTTACAGAGTTAGGGATTTTAGAGATGACACGTAAACGTAAGAAACATTCTTTACGCGACGTATTAC
TAGAAGAGTGTGCACCGTGCAAAGCGACGGGGTATGTAATGTCATATGAAACAATTGCGTATGAGCTAGAGAGAGAGCTA
CTTACATATGGCAATATAGAAGATGAGGCGGTATTAATCTCTGCACCTAAAGAACTGCAAAAACAATTTTTGCAAAAAGA
ATTACAAAAAAATATTCCATTTGAAATTTATTTCAAAGATGATATGATCGAAAAGTATGCAATTATCCGTTTTGGAAGTA
AAAAAGAAATTGTAGAACGGAAAAATAGTTAG

Upstream 100 bases:

>100_bases
ACAACTTCATCTGTTGAAGAATTAATCGGTTAGTGTTGACGTTAGCACTAACCTTTTATTATTGTAAAAGCAAAGAAGAT
TATAAAGTGAGGAAAGAATT

Downstream 100 bases:

>100_bases
TAGAACTTTCATTGACAACCGTCTATGATTCATGATAACATCTTTATGTTATAGTTTATAGCACCTAAACGGCTATATGC
TACAACCGCACAAGACAGGT

Product: ribonuclease

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKTLYMNYAGSEKRVAIEEKKKIVEFLWKRNEEQEIVGHIYIGRIVRTIAGMNAAFVNIGLEKHAYLSYDDVPSAYRIHE
GQAVLVQVVKEAIDTKGPKLTANIEFTGKYVVYMPYDEMRAVSRKIKNNKRRQQLLQIEVEGTGGYIFRSASEKGEIEEI
QAEMQRLQQLYEELKRKEEQGKAPLLLHRPATFLDRVFQENPIETIEKVVVDTRSIVKELEEKVGKEKVSFYNEKSSMFN
HFGIEREIEKALQKIVWLPNGAYLIVEQMETMTVIDVNTGKFTGKQNLQDTVLRTNELAAEEIARQLRLRDIGGMILIDF
INMKRREDKEKVRECLVAAMQNDRTYTRVLGFTELGILEMTRKRKKHSLRDVLLEECAPCKATGYVMSYETIAYELEREL
LTYGNIEDEAVLISAPKELQKQFLQKELQKNIPFEIYFKDDMIEKYAIIRFGSKKEIVERKNS

Sequences:

>Translated_463_residues
MKTLYMNYAGSEKRVAIEEKKKIVEFLWKRNEEQEIVGHIYIGRIVRTIAGMNAAFVNIGLEKHAYLSYDDVPSAYRIHE
GQAVLVQVVKEAIDTKGPKLTANIEFTGKYVVYMPYDEMRAVSRKIKNNKRRQQLLQIEVEGTGGYIFRSASEKGEIEEI
QAEMQRLQQLYEELKRKEEQGKAPLLLHRPATFLDRVFQENPIETIEKVVVDTRSIVKELEEKVGKEKVSFYNEKSSMFN
HFGIEREIEKALQKIVWLPNGAYLIVEQMETMTVIDVNTGKFTGKQNLQDTVLRTNELAAEEIARQLRLRDIGGMILIDF
INMKRREDKEKVRECLVAAMQNDRTYTRVLGFTELGILEMTRKRKKHSLRDVLLEECAPCKATGYVMSYETIAYELEREL
LTYGNIEDEAVLISAPKELQKQFLQKELQKNIPFEIYFKDDMIEKYAIIRFGSKKEIVERKNS
>Mature_463_residues
MKTLYMNYAGSEKRVAIEEKKKIVEFLWKRNEEQEIVGHIYIGRIVRTIAGMNAAFVNIGLEKHAYLSYDDVPSAYRIHE
GQAVLVQVVKEAIDTKGPKLTANIEFTGKYVVYMPYDEMRAVSRKIKNNKRRQQLLQIEVEGTGGYIFRSASEKGEIEEI
QAEMQRLQQLYEELKRKEEQGKAPLLLHRPATFLDRVFQENPIETIEKVVVDTRSIVKELEEKVGKEKVSFYNEKSSMFN
HFGIEREIEKALQKIVWLPNGAYLIVEQMETMTVIDVNTGKFTGKQNLQDTVLRTNELAAEEIARQLRLRDIGGMILIDF
INMKRREDKEKVRECLVAAMQNDRTYTRVLGFTELGILEMTRKRKKHSLRDVLLEECAPCKATGYVMSYETIAYELEREL
LTYGNIEDEAVLISAPKELQKQFLQKELQKNIPFEIYFKDDMIEKYAIIRFGSKKEIVERKNS

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=425, Percent_Identity=35.0588235294118, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1787325, Length=425, Percent_Identity=29.8823529411765, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 53924; Mature: 53924

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLYMNYAGSEKRVAIEEKKKIVEFLWKRNEEQEIVGHIYIGRIVRTIAGMNAAFVNIG
CCEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEC
LEKHAYLSYDDVPSAYRIHEGQAVLVQVVKEAIDTKGPKLTANIEFTGKYVVYMPYDEMR
CCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEEEEECEEEEEECHHHHH
AVSRKIKNNKRRQQLLQIEVEGTGGYIFRSASEKGEIEEIQAEMQRLQQLYEELKRKEEQ
HHHHHHHCCHHHHEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GKAPLLLHRPATFLDRVFQENPIETIEKVVVDTRSIVKELEEKVGKEKVSFYNEKSSMFN
CCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
HFGIEREIEKALQKIVWLPNGAYLIVEQMETMTVIDVNTGKFTGKQNLQDTVLRTNELAA
HCCCHHHHHHHHHHHEECCCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
EEIARQLRLRDIGGMILIDFINMKRREDKEKVRECLVAAMQNDRTYTRVLGFTELGILEM
HHHHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHH
TRKRKKHSLRDVLLEECAPCKATGYVMSYETIAYELERELLTYGNIEDEAVLISAPKELQ
HHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
KQFLQKELQKNIPFEIYFKDDMIEKYAIIRFGSKKEIVERKNS
HHHHHHHHHHCCCEEEEECCHHHHHHHHEEECCHHHHHHCCCC
>Mature Secondary Structure
MKTLYMNYAGSEKRVAIEEKKKIVEFLWKRNEEQEIVGHIYIGRIVRTIAGMNAAFVNIG
CCEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEC
LEKHAYLSYDDVPSAYRIHEGQAVLVQVVKEAIDTKGPKLTANIEFTGKYVVYMPYDEMR
CCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEEEEECEEEEEECHHHHH
AVSRKIKNNKRRQQLLQIEVEGTGGYIFRSASEKGEIEEIQAEMQRLQQLYEELKRKEEQ
HHHHHHHCCHHHHEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GKAPLLLHRPATFLDRVFQENPIETIEKVVVDTRSIVKELEEKVGKEKVSFYNEKSSMFN
CCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
HFGIEREIEKALQKIVWLPNGAYLIVEQMETMTVIDVNTGKFTGKQNLQDTVLRTNELAA
HCCCHHHHHHHHHHHEECCCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
EEIARQLRLRDIGGMILIDFINMKRREDKEKVRECLVAAMQNDRTYTRVLGFTELGILEM
HHHHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHH
TRKRKKHSLRDVLLEECAPCKATGYVMSYETIAYELERELLTYGNIEDEAVLISAPKELQ
HHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
KQFLQKELQKNIPFEIYFKDDMIEKYAIIRFGSKKEIVERKNS
HHHHHHHHHHCCCEEEEECCHHHHHHHHEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]