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The map label for this gene is catB [H]

Identifier: 255961275

GI number: 255961275

Start: 2680944

End: 2682077

Strand: Direct

Name: catB [H]

Synonym: Pfl01_2325

Alternate gene names: 255961275

Gene position: 2680944-2682077 (Clockwise)

Preceding gene: 77458551

Following gene: 77458553

Centisome position: 41.64

GC content: 63.93

Gene sequence:

>1134_bases
ATGGACACTGAAATGAACCGGATCCTGATTGAAAACCTGTCGGCGATCATCGTCGACCTTCCGACTATCCGCCCGCACAA
ACTGGCGATGCACACGATGCAGAACCAGACGCTGGTGATCCTGCGCCTGCGTTGCAGCGACGGTGTCGAAGGCATCGGCG
AAGCCACCACCATCGGCGGCCTGGCCTACGGCTATGAAAGCCCGGAAAGCATCAAGGCCAACATCGATGCGCACCTGGCG
CCGGCGCTGATCGGCATGGACGCGGACAACATCAACGTCGCCATGCAGAAGCTCGACAAGATTGCCAAGGGCAACACCTT
CGCCAAGTCCGCCATCGAGAGCGCCTTGCTCGACGCCCAAGGCAAACGCCTCGGCCTGCCGGTGAGCGAACTGCTCGGCG
GTCGGGTCCGCGACAGCCTCGAAGTGGCCTGGACCCTGGCCAGCGGCGACACCGCCCGCGACATCGCCGAGGCCGAACAA
ATGCTGGAAGCGCGGCGTCACCGGATCTTCAAACTGAAGATCGGCGCCAACCCGCTGGAGCAGGATCTGAAACACGTGGT
GGCGATCAAGAAAGCCCTGGGCGAGCGTGCCAGTGTGCGGGTCGACGTCAACCAGTATTGGGACGAGTCCCAGGCAATTC
GCGGCTGCCGGGTGCTCGGCGACAACGGTATCGACCTGATCGAGCAGCCGATCTCGCGAGTCAATCGTTCCGGTCAGATT
CGTCTGAACCAGCGCAGCCTGGCACCGATCATGGCCGATGAATCCATCGAAAGCGTCGAGGACGCGTTCAGCCTCGCCGC
CGACGGCGCCGCCAGCGTCTTCGCCCTGAAGATCGCCAAGAACGGCGGCCCGCGCGCCGTGTTGCGCACTGCGCAAATCG
CCGAAGCCGCCGGCATCGCGCTGTACGGCGGCACCATGCTCGAAGGCTCCGTCGGCACGCTGGCCTCGGCCCATGCGTTC
CTCACGCTCAGGCAATTGACCTGGGACACCGAGCTGTTTGGTCCGCTGTTGCTCACCGAAGACATCGTCACCGAGCGCCC
GCAGTACCGCGACTTTCACCTGCACATTCCACGCACCCCGGGCCTGGGCCTGACACTGGATGAAGAGCGTCTGGCGCGTT
TTCGCCGGCACTGA

Upstream 100 bases:

>100_bases
CAGGTCGGCGCGATCCTGTTTCTCGCCTCGGACGACGCCTCCTACATCACCGGTGTAACCCTGCCGGTCGGTGGCGGAGA
CCTCGGTTGATCACGCTTGA

Downstream 100 bases:

>100_bases
CCCCTAAGGAGAACGCCATGCTGTTCCACGTAAAAATGACCGTGAAATTGCCCGCCGACATGGATCAGGCCGTTGCCACA
AAACTCAAGGCCGACGAAAA

Product: muconate cycloisomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDTEMNRILIENLSAIIVDLPTIRPHKLAMHTMQNQTLVILRLRCSDGVEGIGEATTIGGLAYGYESPESIKANIDAHLA
PALIGMDADNINVAMQKLDKIAKGNTFAKSAIESALLDAQGKRLGLPVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQ
MLEARRHRIFKLKIGANPLEQDLKHVVAIKKALGERASVRVDVNQYWDESQAIRGCRVLGDNGIDLIEQPISRVNRSGQI
RLNQRSLAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGGTMLEGSVGTLASAHAF
LTLRQLTWDTELFGPLLLTEDIVTERPQYRDFHLHIPRTPGLGLTLDEERLARFRRH

Sequences:

>Translated_377_residues
MDTEMNRILIENLSAIIVDLPTIRPHKLAMHTMQNQTLVILRLRCSDGVEGIGEATTIGGLAYGYESPESIKANIDAHLA
PALIGMDADNINVAMQKLDKIAKGNTFAKSAIESALLDAQGKRLGLPVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQ
MLEARRHRIFKLKIGANPLEQDLKHVVAIKKALGERASVRVDVNQYWDESQAIRGCRVLGDNGIDLIEQPISRVNRSGQI
RLNQRSLAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGGTMLEGSVGTLASAHAF
LTLRQLTWDTELFGPLLLTEDIVTERPQYRDFHLHIPRTPGLGLTLDEERLARFRRH
>Mature_377_residues
MDTEMNRILIENLSAIIVDLPTIRPHKLAMHTMQNQTLVILRLRCSDGVEGIGEATTIGGLAYGYESPESIKANIDAHLA
PALIGMDADNINVAMQKLDKIAKGNTFAKSAIESALLDAQGKRLGLPVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQ
MLEARRHRIFKLKIGANPLEQDLKHVVAIKKALGERASVRVDVNQYWDESQAIRGCRVLGDNGIDLIEQPISRVNRSGQI
RLNQRSLAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGGTMLEGSVGTLASAHAF
LTLRQLTWDTELFGPLLLTEDIVTERPQYRDFHLHIPRTPGLGLTLDEERLARFRRH

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=309, Percent_Identity=26.8608414239482, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 41116; Mature: 41116

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTEMNRILIENLSAIIVDLPTIRPHKLAMHTMQNQTLVILRLRCSDGVEGIGEATTIGG
CCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHCC
LAYGYESPESIKANIDAHLAPALIGMDADNINVAMQKLDKIAKGNTFAKSAIESALLDAQ
EEECCCCCCHHEECCCCHHHHHEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GKRLGLPVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLEARRHRIFKLKIGANPLE
CCCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEEECCCHHH
QDLKHVVAIKKALGERASVRVDVNQYWDESQAIRGCRVLGDNGIDLIEQPISRVNRSGQI
HHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHCCCCCEE
RLNQRSLAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIA
EECCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEE
LYGGTMLEGSVGTLASAHAFLTLRQLTWDTELFGPLLLTEDIVTERPQYRDFHLHIPRTP
EECCEEECCCHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCCCCCEEEEECCCCC
GLGLTLDEERLARFRRH
CCCEEECHHHHHHHHCC
>Mature Secondary Structure
MDTEMNRILIENLSAIIVDLPTIRPHKLAMHTMQNQTLVILRLRCSDGVEGIGEATTIGG
CCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHCC
LAYGYESPESIKANIDAHLAPALIGMDADNINVAMQKLDKIAKGNTFAKSAIESALLDAQ
EEECCCCCCHHEECCCCHHHHHEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GKRLGLPVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLEARRHRIFKLKIGANPLE
CCCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEEECCCHHH
QDLKHVVAIKKALGERASVRVDVNQYWDESQAIRGCRVLGDNGIDLIEQPISRVNRSGQI
HHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHCCCCCEE
RLNQRSLAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIA
EECCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEE
LYGGTMLEGSVGTLASAHAFLTLRQLTWDTELFGPLLLTEDIVTERPQYRDFHLHIPRTP
EECCEEECCCHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCCCCCEEEEECCCCC
GLGLTLDEERLARFRRH
CCCEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3609743; 2449420; 2215699; 3612800; 7500361 [H]