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The map label for this gene is acnB [H]

Identifier: 255961273

GI number: 255961273

Start: 3871434

End: 3874058

Strand: Direct

Name: acnB [H]

Synonym: Pfl01_3395

Alternate gene names: 255961273

Gene position: 3871434-3874058 (Clockwise)

Preceding gene: 77459615

Following gene: 77459620

Centisome position: 60.13

GC content: 60.8

Gene sequence:

>2625_bases
ATGAGGGTAATAACTGTGCTTGAAGCCTACCGCAAACATATCGAAGAGCGTGCAGCCCTGGGTATCGTTCCCCAGCCGCT
TAACGCCGAACAAACTGCAGGCCTGGTCGAGCTGCTGAAGAATCCTCCGGCTGGCGAAGAAGCTTTCCTCGTTGACCTGA
TCACCAATCGCGTACCACCAGGAGTGGACGAAGCCGCCTACGTCAAGGCCGGTTTCCTCTCCGCACTGGCCAAGGGCGAA
GTCTCCTCCCCTCTGCTGGACAAGAAGCGCGCTGTAGAACTGCTCGGCACCATGCAGGGCGGCTACAACATCGTGACCCT
GGTTGAACTGCTGGACGACGCCGAGCTGGCGCCAGTGGCCGCCGAAGAACTCAAGCACACCCTGCTGATGTTCGATGCCT
TCCACGACGTGGCTGAAAAAGCCAAGAACGGCAACGTTCACGCCAAGGCCGTGCTGCAATCCTGGGCTGACGGCGAGTGG
TTCAAGAAGCGCCCTGTACTGGCCGACAAGATCAGCCTGCGCGTCTTCAAGGTCACCGGCGAAACCAACACCGACGACCT
GTCCCCTGCTCCAGACGCCTGGTCGCGTCCTGACATCCCGCTGCACGCCCTGGCCATGCTGAAAATGGCCCGTGACGGTA
TCGTTCCGGACGAGCAAGGCAAGACCGGCCCGATGAAGCAGATCGAAGAAATGCGCGGCCAGGGCTTCCCTATCGCCTAC
GTCGGTGACGTGGTCGGTACCGGTTCCTCGCGTAAATCGGCGACCAACTCGGTGCTGTGGTTCTTCGGCGACGACATCCC
TTACGTGCCGAACAAGCGCGCTGGCGGTTTCTGCTTCGGCAGCAAAATCGCTCCGATCTTCTACAACACCATGGAAGATG
CCGGCGCACTGCCAATCGAGTTCGACGTATCGAACATGAACATGGGCGACGTGATCGACCTATACCCGCACGCTGGCAAA
GTCTGCAAGCACGGTACCGACGAAGTCATCACCACCTTCGAAATGAAGACCCCGGTCCTGCTGGACGAAGTCCGCGCCGG
CGGCCGTATCCCGCTGATCATCGGCCGTGGCCTGACCGAGAAGGCTCGCGCCGAACTGGGTCTGCCAGCGTTCGACCTGT
TCAAGAAGCCGGAAGCACCGGCTGAAAGCACCAAGGGCTTCACCCTGGCGCAGAAAATGGTCGGCAAGGCTTGCGGTCTG
GCAGAAGGCAAAGGCGTTCGTCCTGGCACCTACTGCGAACCGAAGATGACCACCGTAGGTTCTCAGGACACCACCGGTCC
AATGACCCGTGACGAACTGAAAGATCTGGCGTGCCTGGGCTTCTCCGCTGATCTGGTGATGCAGTCCTTCTGCCACACCG
CGGCTTATCCAAAGCCGATCGACGTGACCACCCACCACACCCTGCCTGACTTCATCATGACCCGCGGCGGTGTTTCCCTG
CGTCCGGGCGACGGCATCATCCACTCGTGGCTGAACCGCATGCTGCTGCCGGACACCGTCGGTACCGGTGGTGACTCGCA
CACCCGTTTCCCGATGGGCATCTCGTTCCCGGCCGGTTCCGGTCTGGTCGCGTTCGCCGCTGCCACCGGCGTCATGCCGC
TGGACATGCCGGAGTCGATCCTGGTGCGCTTCAAAGGCAAAATGAAACCTGGCATCACCCTGCGTGACCTGGTTCATGCC
ATTCCTTACTTCGCCATCCAGAACGGTCTGCTGACCGTCGAGAAGAAAGGCAAGAAAAACGCCTTCTCCGGCCGCATCCT
GGAAATCGAAGGCCTGGAAGGTCTGACCCTGGAACAGGCGTTCGAACTGTCCGACGCCTCGGCCGAACGTTCGGCTGCCG
GTTGCACCATCAAGCTGTCGAAAGAGTCGATCACCGAGTACCTGCAGTCCAACATCACCCTGCTGCGCTGGATGATCGGC
GAAGGCTACGGCGATGCCCGCACCCTGGAACGTCGTGCTCAAGCGATGGAAGCCTGGATCGCCAACCCGCAACTGATGGA
AGCCGATGCCGACGCCGAATACGCCGAAGTCATCGAAATCGATCTGGCCGACATCAGCGAGCCTGTACTGTGCGCGCCGA
ACGATCCGGACGACGCCCGTCTGCTGTCCAGCGTTGCTGGCGAGAAGATCGACGAAGTGTTCATCGGTTCGTGCATGACC
AACATCGGTCACTTCCGCGCTGCCGGTAAACTGCTGGATCAGGTCAAGGGTCAGCTGCCAACCCGTCTGTGGCTGTCGCC
ACCGACCAAGATGGACGCTCACCAACTGACCGAAGAAGGCTACTACGGCATCTACGGCAAGGCCGGCGCCCGCATGGAAA
TGCCAGGCTGCTCGCTGTGCATGGGTAACCAGGCACGCGTTGAGCCGAACAGCACCGTGGTGTCGACCTCGACCCGTAAC
TTCCCGAACCGTCTGGGCGACGGCGCGAACGTCTACCTGGCCTCGGCTGAGCTGGCGTCCGTTGCTTCGATCCTGGGTCG
CCTGCCGACCGTCGAGGAGTACATGGAATACGCTGGCAAGATCGACAGCATGGCGGCCGACGTTTACCGCTACCTGTCCT
TCGACCAGATTGCCGAGTTCCGTGAAGCTGCTGCGAACGCCAAGATCCCTGTCGTTCAAGCCTAA

Upstream 100 bases:

>100_bases
GAATGCCCGGTAATTCTGGCTAAGCGGCCCACAACGCCACGCAGCGCATGAGTACGAGCATTTCGGGATGAGCGTCCCGG
ACACCCATTTGGGACCACTG

Downstream 100 bases:

>100_bases
CGCTGCAACGCAATGAAGAACGCCGCCCATCGCGAGATGAGCGGCGTTTTTTTATGCCTGCTGAACGATCAATCACAAAC
CATACAGGCACAAAAAAGGC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 874; Mature: 874

Protein sequence:

>874_residues
MRVITVLEAYRKHIEERAALGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEAAYVKAGFLSALAKGE
VSSPLLDKKRAVELLGTMQGGYNIVTLVELLDDAELAPVAAEELKHTLLMFDAFHDVAEKAKNGNVHAKAVLQSWADGEW
FKKRPVLADKISLRVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDEQGKTGPMKQIEEMRGQGFPIAY
VGDVVGTGSSRKSATNSVLWFFGDDIPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSNMNMGDVIDLYPHAGK
VCKHGTDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTEKARAELGLPAFDLFKKPEAPAESTKGFTLAQKMVGKACGL
AEGKGVRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPIDVTTHHTLPDFIMTRGGVSL
RPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKMKPGITLRDLVHA
IPYFAIQNGLLTVEKKGKKNAFSGRILEIEGLEGLTLEQAFELSDASAERSAAGCTIKLSKESITEYLQSNITLLRWMIG
EGYGDARTLERRAQAMEAWIANPQLMEADADAEYAEVIEIDLADISEPVLCAPNDPDDARLLSSVAGEKIDEVFIGSCMT
NIGHFRAAGKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVEPNSTVVSTSTRN
FPNRLGDGANVYLASAELASVASILGRLPTVEEYMEYAGKIDSMAADVYRYLSFDQIAEFREAAANAKIPVVQA

Sequences:

>Translated_874_residues
MRVITVLEAYRKHIEERAALGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEAAYVKAGFLSALAKGE
VSSPLLDKKRAVELLGTMQGGYNIVTLVELLDDAELAPVAAEELKHTLLMFDAFHDVAEKAKNGNVHAKAVLQSWADGEW
FKKRPVLADKISLRVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDEQGKTGPMKQIEEMRGQGFPIAY
VGDVVGTGSSRKSATNSVLWFFGDDIPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSNMNMGDVIDLYPHAGK
VCKHGTDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTEKARAELGLPAFDLFKKPEAPAESTKGFTLAQKMVGKACGL
AEGKGVRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPIDVTTHHTLPDFIMTRGGVSL
RPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKMKPGITLRDLVHA
IPYFAIQNGLLTVEKKGKKNAFSGRILEIEGLEGLTLEQAFELSDASAERSAAGCTIKLSKESITEYLQSNITLLRWMIG
EGYGDARTLERRAQAMEAWIANPQLMEADADAEYAEVIEIDLADISEPVLCAPNDPDDARLLSSVAGEKIDEVFIGSCMT
NIGHFRAAGKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVEPNSTVVSTSTRN
FPNRLGDGANVYLASAELASVASILGRLPTVEEYMEYAGKIDSMAADVYRYLSFDQIAEFREAAANAKIPVVQA
>Mature_874_residues
MRVITVLEAYRKHIEERAALGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEAAYVKAGFLSALAKGE
VSSPLLDKKRAVELLGTMQGGYNIVTLVELLDDAELAPVAAEELKHTLLMFDAFHDVAEKAKNGNVHAKAVLQSWADGEW
FKKRPVLADKISLRVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDEQGKTGPMKQIEEMRGQGFPIAY
VGDVVGTGSSRKSATNSVLWFFGDDIPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSNMNMGDVIDLYPHAGK
VCKHGTDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTEKARAELGLPAFDLFKKPEAPAESTKGFTLAQKMVGKACGL
AEGKGVRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPIDVTTHHTLPDFIMTRGGVSL
RPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKMKPGITLRDLVHA
IPYFAIQNGLLTVEKKGKKNAFSGRILEIEGLEGLTLEQAFELSDASAERSAAGCTIKLSKESITEYLQSNITLLRWMIG
EGYGDARTLERRAQAMEAWIANPQLMEADADAEYAEVIEIDLADISEPVLCAPNDPDDARLLSSVAGEKIDEVFIGSCMT
NIGHFRAAGKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVEPNSTVVSTSTRN
FPNRLGDGANVYLASAELASVASILGRLPTVEEYMEYAGKIDSMAADVYRYLSFDQIAEFREAAANAKIPVVQA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=859, Percent_Identity=77.8812572759022, Blast_Score=1375, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=380, Percent_Identity=26.0526315789474, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322261, Length=400, Percent_Identity=26.75, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94522; Mature: 94522

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVITVLEAYRKHIEERAALGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPP
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
GVDEAAYVKAGFLSALAKGEVSSPLLDKKRAVELLGTMQGGYNIVTLVELLDDAELAPVA
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHH
AEELKHTLLMFDAFHDVAEKAKNGNVHAKAVLQSWADGEWFKKRPVLADKISLRVFKVTG
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCCCEEEEEEEEEEEEC
ETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDEQGKTGPMKQIEEMRGQGFPIAY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEE
VGDVVGTGSSRKSATNSVLWFFGDDIPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIE
ECCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEE
FDVSNMNMGDVIDLYPHAGKVCKHGTDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTE
EECCCCCCCCEEECCCCCCHHHCCCCCHHEEEEECCCCHHHHHHCCCCCEEEEEECCCCH
KARAELGLPAFDLFKKPEAPAESTKGFTLAQKMVGKACGLAEGKGVRPGTYCEPKMTTVG
HHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECC
SQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPIDVTTHHTLPDFIMTRGGVSL
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEE
RPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESI
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCEEHHHHCCCCCCCCCHHH
LVRFKGKMKPGITLRDLVHAIPYFAIQNGLLTVEKKGKKNAFSGRILEIEGLEGLTLEQA
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHH
FELSDASAERSAAGCTIKLSKESITEYLQSNITLLRWMIGEGYGDARTLERRAQAMEAWI
HHCCCCCCHHCCCCCEEEECHHHHHHHHHCCHHEEHHHHHCCCCHHHHHHHHHHHHHHHH
ANPQLMEADADAEYAEVIEIDLADISEPVLCAPNDPDDARLLSSVAGEKIDEVFIGSCMT
CCCCEEECCCCCCCHHHEEEEHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NIGHFRAAGKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLC
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCEEEECCCCCEEECCCCCEE
MGNQARVEPNSTVVSTSTRNFPNRLGDGANVYLASAELASVASILGRLPTVEEYMEYAGK
ECCCCEECCCCEEEECCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHCC
IDSMAADVYRYLSFDQIAEFREAAANAKIPVVQA
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MRVITVLEAYRKHIEERAALGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPP
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
GVDEAAYVKAGFLSALAKGEVSSPLLDKKRAVELLGTMQGGYNIVTLVELLDDAELAPVA
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHH
AEELKHTLLMFDAFHDVAEKAKNGNVHAKAVLQSWADGEWFKKRPVLADKISLRVFKVTG
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCCCEEEEEEEEEEEEC
ETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDEQGKTGPMKQIEEMRGQGFPIAY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEE
VGDVVGTGSSRKSATNSVLWFFGDDIPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIE
ECCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEE
FDVSNMNMGDVIDLYPHAGKVCKHGTDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTE
EECCCCCCCCEEECCCCCCHHHCCCCCHHEEEEECCCCHHHHHHCCCCCEEEEEECCCCH
KARAELGLPAFDLFKKPEAPAESTKGFTLAQKMVGKACGLAEGKGVRPGTYCEPKMTTVG
HHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECC
SQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPIDVTTHHTLPDFIMTRGGVSL
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEE
RPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESI
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCEEHHHHCCCCCCCCCHHH
LVRFKGKMKPGITLRDLVHAIPYFAIQNGLLTVEKKGKKNAFSGRILEIEGLEGLTLEQA
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHH
FELSDASAERSAAGCTIKLSKESITEYLQSNITLLRWMIGEGYGDARTLERRAQAMEAWI
HHCCCCCCHHCCCCCEEEECHHHHHHHHHCCHHEEHHHHHCCCCHHHHHHHHHHHHHHHH
ANPQLMEADADAEYAEVIEIDLADISEPVLCAPNDPDDARLLSSVAGEKIDEVFIGSCMT
CCCCEEECCCCCCCHHHEEEEHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NIGHFRAAGKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLC
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCEEEECCCCCEEECCCCCEE
MGNQARVEPNSTVVSTSTRNFPNRLGDGANVYLASAELASVASILGRLPTVEEYMEYAGK
ECCCCEECCCCEEEECCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHCC
IDSMAADVYRYLSFDQIAEFREAAANAKIPVVQA
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]