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The map label for this gene is ywqL

Identifier: 255767779

GI number: 255767779

Start: 3724420

End: 3725136

Strand: Reverse

Name: ywqL

Synonym: BSU36170

Alternate gene names: 255767779

Gene position: 3725136-3724420 (Counterclockwise)

Preceding gene: 16080671

Following gene: 16080668

Centisome position: 88.37

GC content: 46.86

Gene sequence:

>717_bases
ATGAAGGTATTTGATGTGCATAAATTTGATATGAAAAAGGAACAAGACTTTTTACAAGTGCAATTTAACCTTAAAAATCG
TATCAACCTATCCCCCACGATCCATCCAGACTCCATCAACACCTGCGCAGGCGTCGACCTGGCCTATTGGGAGCAGGATG
GAGAACCATACGGAGTGTGCTGTATTATCGTAATTGACGCTGATACAAAAGAAGTCATTGAAAAAGTGCACAGCATGGGG
AGAATCAGCGTGCCGTATGTTTCCGGTTTTCTTGCGTTTCGCGAGCTGCCCTTGATCATCGAGGCGGCGAAAAAGCTGGA
GACGGAGCCGGATGTCTTCTTGTTTGATGGCAACGGATATCTGCATTACAATCATATGGGCGTTGCCACCCATGCGGCTT
TTTTTCTGGGCAAGCCGACGATTGGGATAGCGAAAACCTATCTCAAAATCAAAGGCTGCGATTTCGTGACGCCTGAAATT
GAAGTAGGCGCATACACCGATATTATCATTGACGGCGAGGTATATGGACGGGCGCTGCGAACGCGGCGGGACGTGAAGCC
TATTTTCTTGTCTTGCGGTAATTATATTGATTTAGACAGCAGCTATCAGATCACGATGAGCTTGATCAATCAAGAAAGCA
GGCTGCCGATCCCTGTCCGCCTGGCTGACCTTGAGACGCATGTTTTGCGAACGTTTTATCAGAAAAATCACGTGTAA

Upstream 100 bases:

>100_bases
ATTAAATAAGTACGAGATAGATACAGATAGTCCTCATTTTGAAATACTAGAATCGAGAAGAGAGACTCACATTAATTTAG
GTCGAGAGTGAGCAATCGTT

Downstream 100 bases:

>100_bases
CACTTGGGGCTCTAAGTCATCAAGTGTTTTTTTTGTATGTGTGTGCCCTGGTCTCAAAAACCTTGTCGTCCTTGTGGAGA
GGTTTTATTTGAATGTTTTC

Product: deoxyribonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIHPDSINTCAGVDLAYWEQDGEPYGVCCIIVIDADTKEVIEKVHSMG
RISVPYVSGFLAFRELPLIIEAAKKLETEPDVFLFDGNGYLHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVTPEI
EVGAYTDIIIDGEVYGRALRTRRDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV

Sequences:

>Translated_238_residues
MKVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIHPDSINTCAGVDLAYWEQDGEPYGVCCIIVIDADTKEVIEKVHSMG
RISVPYVSGFLAFRELPLIIEAAKKLETEPDVFLFDGNGYLHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVTPEI
EVGAYTDIIIDGEVYGRALRTRRDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV
>Mature_238_residues
MKVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIHPDSINTCAGVDLAYWEQDGEPYGVCCIIVIDADTKEVIEKVHSMG
RISVPYVSGFLAFRELPLIIEAAKKLETEPDVFLFDGNGYLHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVTPEI
EVGAYTDIIIDGEVYGRALRTRRDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=202, Percent_Identity=31.1881188118812, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87082357, Length=206, Percent_Identity=30.0970873786408, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_BACSU (P96724)

Other databases:

- EMBL:   Z92952
- EMBL:   AL009126
- PIR:   G70067
- RefSeq:   NP_391498.2
- PDB:   3GA2
- PDBsum:   3GA2
- ProteinModelPortal:   P96724
- SMR:   P96724
- EnsemblBacteria:   EBBACT00000002711
- GeneID:   936894
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36170
- NMPDR:   fig|224308.1.peg.3624
- GenoList:   BSU36170
- GeneTree:   EBGT00050000000793
- HOGENOM:   HBG716360
- PhylomeDB:   P96724
- ProtClustDB:   CLSK882396
- BioCyc:   BSUB:BSU36170-MONOMER
- BRENDA:   3.1.21.7
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 27023; Mature: 27023

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIHPDSINTCAGVDLAYWEQDGEPYGVC
CCEEECCCCCCCCCCCEEEEEEECCCEECCCCEECCCCCCHHCCCEEEEEECCCCCEEEE
CIIVIDADTKEVIEKVHSMGRISVPYVSGFLAFRELPLIIEAAKKLETEPDVFLFDGNGY
EEEEECCCHHHHHHHHHHCCCEECHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEECCCCE
LHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVTPEIEVGAYTDIIIDGEVYGRALR
EEEECCCHHHHHHHHCCCCCHHEEEEEEEEECCCEECCCEECCCEEEEEECCHHHHHHHH
TRRDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV
HHCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIHPDSINTCAGVDLAYWEQDGEPYGVC
CCEEECCCCCCCCCCCEEEEEEECCCEECCCCEECCCCCCHHCCCEEEEEECCCCCEEEE
CIIVIDADTKEVIEKVHSMGRISVPYVSGFLAFRELPLIIEAAKKLETEPDVFLFDGNGY
EEEEECCCHHHHHHHHHHCCCEECHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEECCCCE
LHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVTPEIEVGAYTDIIIDGEVYGRALR
EEEECCCHHHHHHHHCCCCCHHEEEEEEEEECCCEECCCEECCCEEEEEECCHHHHHHHH
TRRDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV
HHCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377