Click here to switch to the map view.

The map label for this gene is gbsB

Identifier: 255767703

GI number: 255767703

Start: 3183867

End: 3185075

Strand: Reverse

Name: gbsB

Synonym: BSU31050

Alternate gene names: 255767703

Gene position: 3185075-3183867 (Counterclockwise)

Preceding gene: 16080158

Following gene: 16080156

Centisome position: 75.55

GC content: 47.31

Gene sequence:

>1209_bases
ATGACATTAAATATGAAGGTAGAAAGCATGCAGAAATTCCACACATTTGAAATTCCGACTGTGATTAAGCATGGAATCGG
AGCCATCAAGCATACGGGTGAGGAAGTAGCTGCACTGGGCGTTTCAAAAGCGCTCCTTGTCACAGACCCTGGGATTTATA
AAGCCGGTGTTGCTGATCCCGTAATCGAATCACTTAAAGAAGCAGGCATTGAAGTGGTACTCTTTAATAAAGTTGAGCCA
AACCCGCCTGTCCGTCTGGTGAATGAAGGATCTGAGCTTTACAAAAAAGAGAACTGTAATGGTTTGGTGGCAGTCGGAGG
CGGAAGCTCCATGGATACAGCAAAAGCAATCGGAGTAGAAGCGACTCACGAAGGAAGCGTGCTTGATTATGAAGCGGCAG
ATGGGAAAAAACCGCTGGAAAACCGCATTCCTCCGCTGACGACAATTCCCACTACAGCTGGAACCGGATCAGAAGTAACC
CAATGGGCGGTTATCACAGATGAAGAAAGAGAATTCAAATTCAACACGGGCGGTCCGCTGATTGCAGCGCACTTGACAAT
CATTGATCCTGAGCTTCATGTTTCAATGCCTCCGCATGTAACAGCTATGACGGGAATTGATGCGCTCGCCCATGCCATTG
AGTGCTATACAATGAAATTTGCACAGCCAATCACGGATGCGGTTGCCTTAATGGCGATTGAGTATGCCGCTCATTACATC
AAGAGGGCATTTGCTGATGGAGAGGATCTGGAAGCAAGGTACGGAATGGCGCAGGCTGCGATGCTTGCCGGTCTTTCTTA
CGGAAGTGAATCAGCGGGCGCTGCTCATGCGATGAGCCAAACGCTTGGCGGTATTATCCCAGTCGCTCACGGCCAATGTG
TAGCGGCGATGATGGGCCCTGTCATGGAATACAACTGGAAGGGCTATCCTGAGAAGTTTGCGCGTATTGCGAAAGCGTTC
GGTATTGATACAAGCAAGATGACAACAGAAGAAGCTGCGAAAGCATCTGTCAATTGGATGTATGATCTGGTCGAAGATTT
AGAAGTGCCGACTTTAGAAGAGCAGGGAGTATCACCTGATATGATCGAACGCTTGTCTAAAGAAGCAATGAAAGATCCGC
AAACCTTCGGAAACCCTAGAGATTTAAATGAAAAAGCATACAACTGGATCTACAAACGCTGCTTTAACCTTACGCCTAAA
ACGGTATAA

Upstream 100 bases:

>100_bases
AAAATAGGCCTTGAAGAATACACAGAAGTCAAACATGTATACCGCAATACAAAACCGGCAGCGGTTAACTGGTTTAATTC
ATAAGAGGGGGAGATTGATG

Downstream 100 bases:

>100_bases
TCAAAATCCCGCTCCTTTACAGAGCGGGATTTTTTGGGATAATGAAGCATAGGTGGAAATTTTAGATAAGAAGGAAGGTG
CCAGCTGATGTGGAAGCGTA

Product: choline dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTLNMKVESMQKFHTFEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADPVIESLKEAGIEVVLFNKVEP
NPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAADGKKPLENRIPPLTTIPTTAGTGSEVT
QWAVITDEEREFKFNTGGPLIAAHLTIIDPELHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYI
KRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPEKFARIAKAF
GIDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPDMIERLSKEAMKDPQTFGNPRDLNEKAYNWIYKRCFNLTPK
TV

Sequences:

>Translated_402_residues
MTLNMKVESMQKFHTFEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADPVIESLKEAGIEVVLFNKVEP
NPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAADGKKPLENRIPPLTTIPTTAGTGSEVT
QWAVITDEEREFKFNTGGPLIAAHLTIIDPELHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYI
KRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPEKFARIAKAF
GIDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPDMIERLSKEAMKDPQTFGNPRDLNEKAYNWIYKRCFNLTPK
TV
>Mature_401_residues
TLNMKVESMQKFHTFEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADPVIESLKEAGIEVVLFNKVEPN
PPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAADGKKPLENRIPPLTTIPTTAGTGSEVTQ
WAVITDEEREFKFNTGGPLIAAHLTIIDPELHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYIK
RAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPEKFARIAKAFG
IDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPDMIERLSKEAMKDPQTFGNPRDLNEKAYNWIYKRCFNLTPKT
V

Specific function: Essential for the utilization of choline as a precursor

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family

Homologues:

Organism=Homo sapiens, GI133922590, Length=363, Percent_Identity=30.3030303030303, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI48994951, Length=380, Percent_Identity=35.2631578947368, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1789163, Length=358, Percent_Identity=35.7541899441341, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI87082107, Length=384, Percent_Identity=30.7291666666667, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787493, Length=393, Percent_Identity=29.2620865139949, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789386, Length=401, Percent_Identity=25.1870324189526, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=421, Percent_Identity=26.3657957244656, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321181, Length=397, Percent_Identity=33.2493702770781, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24657991, Length=438, Percent_Identity=22.8310502283105, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GBSB_BACSU (P71017)

Other databases:

- EMBL:   U47861
- EMBL:   AL009126
- PIR:   B69629
- RefSeq:   NP_390983.2
- ProteinModelPortal:   P71017
- SMR:   P71017
- EnsemblBacteria:   EBBACT00000003100
- GeneID:   938832
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31050
- NMPDR:   fig|224308.1.peg.3108
- GenoList:   BSU31050
- GeneTree:   EBGT00050000000021
- HOGENOM:   HBG742415
- OMA:   IDPELHV
- PhylomeDB:   P71017
- ProtClustDB:   CLSK887778
- BioCyc:   BSUB:BSU31050-MONOMER
- BioCyc:   MetaCyc:MONOMER-8601
- BRENDA:   1.1.1.1
- InterPro:   IPR001670
- InterPro:   IPR018211

Pfam domain/function: PF00465 Fe-ADH

EC number: =1.1.1.1

Molecular weight: Translated: 43421; Mature: 43290

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00913 ADH_IRON_1; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNMKVESMQKFHTFEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADP
CEECCCHHHHHHHHCCCCHHHHHHCCHHHHHCCHHHHHHCCCCEEEEECCCCCCCCCCHH
VIESLKEAGIEVVLFNKVEPNPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVE
HHHHHHHCCCEEEEEECCCCCCCEEEECCCHHHHHHCCCCEEEEECCCCCCCHHHHHCCE
ATHEGSVLDYEAADGKKPLENRIPPLTTIPTTAGTGSEVTQWAVITDEEREFKFNTGGPL
ECCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCE
IAAHLTIIDPELHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYI
EEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEECCCCHHHHHHHHH
VMEYNWKGYPEKFARIAKAFGIDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPD
HHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
MIERLSKEAMKDPQTFGNPRDLNEKAYNWIYKRCFNLTPKTV
HHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TLNMKVESMQKFHTFEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADP
EECCCHHHHHHHHCCCCHHHHHHCCHHHHHCCHHHHHHCCCCEEEEECCCCCCCCCCHH
VIESLKEAGIEVVLFNKVEPNPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVE
HHHHHHHCCCEEEEEECCCCCCCEEEECCCHHHHHHCCCCEEEEECCCCCCCHHHHHCCE
ATHEGSVLDYEAADGKKPLENRIPPLTTIPTTAGTGSEVTQWAVITDEEREFKFNTGGPL
ECCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCE
IAAHLTIIDPELHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYI
EEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEECCCCHHHHHHHHH
VMEYNWKGYPEKFARIAKAFGIDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPD
HHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
MIERLSKEAMKDPQTFGNPRDLNEKAYNWIYKRCFNLTPKTV
HHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8752328; 9384377