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The map label for this gene is glcK

Identifier: 255767559

GI number: 255767559

Start: 2570606

End: 2571571

Strand: Reverse

Name: glcK

Synonym: BSU24850

Alternate gene names: 255767559

Gene position: 2571571-2570606 (Counterclockwise)

Preceding gene: 16079542

Following gene: 255767558

Centisome position: 61.0

GC content: 47.31

Gene sequence:

>966_bases
ATGGACGAGATATGGTTTGCGGGCATTGACCTGGGAGGAACGACGATTAAACTCGCTTTTATTAATCAATATGGCGAAAT
TCAGCATAAGTGGGAAGTTCCGACAGATAAAACCGGCGACACGATTACTGTCACAATTGCAAAAACAATCGACAGCAAGC
TGGATGAGCTGCAAAAACCGAAGCACATCATCAAATACATCGGAATGGGTGCACCAGGCCCTGTAGATATGGCGGCAGGA
GTGGTTTATGAAACAGTAAATCTAGGGTGGAAAAATTATGCTTTGAAAAACCATCTGGAGACAGAAACCGGCATCCCAGC
TGTTATAGAAAATGACGCGAATATTGCTGCGCTCGGGGAAATGTGGAAGGGAGCGGGTGATGGCGCAAAAGACGTCATTC
TCGTGACGCTTGGCACAGGAGTTGGCGGCGGCATCATTGCAAATGGTGAAATTGTACATGGTATAAATGGCGCCGGCGGA
GAAATCGGCCATATTTGCAGCATCCCTGAAGGCGGAGCGCCCTGCAACTGCGGCAAAACGGGCTGTATCGAAACAATTGC
GTCAGCAACCGGAATTGTAAGAATTGCAAAAGAAAAAATAGCAAATGCTAAAAAGACGACACGTTTAAAAGCAACCGAAC
AATTGTCAGCGCGAGATGTGTTTGAAGCGGCGGGTGAAAATGATGAAATTGCCCTTGAGGTGGTTGATTATGTAGCCAAG
CATCTTGGTTTGGTGCTCGGAAATTTGGCAAGCTCGCTTAATCCATCCAAAATCGTTCTTGGCGGCGGCGTATCGAGAGC
CGGAGAACTGCTGAGATCAAAAGTCGAGAAAACATTCCGCAAATGCGCGTTTCCGCGGGCAGCCCAAGCTGCTGATATTT
CAATCGCAGCACTTGGAAATGATGCCGGCGTTATCGGAGGCGCTTGGATCGCTAAAAATGAATGGCTGAAACATCAAAAT
TGTTAA

Upstream 100 bases:

>100_bases
ATTGAAAAGGAGCAGTGGGCCAGAGCGGCAGCTGTCCTTCGAAAAGAATTGGAACAAACAAAAAACGGAAGAGATTTTTA
TAAAGGCTAAGGTGATAAAA

Downstream 100 bases:

>100_bases
AATTGTGTAAATGAAATTGATTTTTTGTTGTGCTCAGGTTAAGATTTAATTTGATGTGTTAATGAGAATGTTGGGAATAG
ACTGATTTTTTTGAGCGTGC

Product: glucose kinase

Products: NA

Alternate protein names: Glucose kinase

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDEIWFAGIDLGGTTIKLAFINQYGEIQHKWEVPTDKTGDTITVTIAKTIDSKLDELQKPKHIIKYIGMGAPGPVDMAAG
VVYETVNLGWKNYALKNHLETETGIPAVIENDANIAALGEMWKGAGDGAKDVILVTLGTGVGGGIIANGEIVHGINGAGG
EIGHICSIPEGGAPCNCGKTGCIETIASATGIVRIAKEKIANAKKTTRLKATEQLSARDVFEAAGENDEIALEVVDYVAK
HLGLVLGNLASSLNPSKIVLGGGVSRAGELLRSKVEKTFRKCAFPRAAQAADISIAALGNDAGVIGGAWIAKNEWLKHQN
C

Sequences:

>Translated_321_residues
MDEIWFAGIDLGGTTIKLAFINQYGEIQHKWEVPTDKTGDTITVTIAKTIDSKLDELQKPKHIIKYIGMGAPGPVDMAAG
VVYETVNLGWKNYALKNHLETETGIPAVIENDANIAALGEMWKGAGDGAKDVILVTLGTGVGGGIIANGEIVHGINGAGG
EIGHICSIPEGGAPCNCGKTGCIETIASATGIVRIAKEKIANAKKTTRLKATEQLSARDVFEAAGENDEIALEVVDYVAK
HLGLVLGNLASSLNPSKIVLGGGVSRAGELLRSKVEKTFRKCAFPRAAQAADISIAALGNDAGVIGGAWIAKNEWLKHQN
C
>Mature_321_residues
MDEIWFAGIDLGGTTIKLAFINQYGEIQHKWEVPTDKTGDTITVTIAKTIDSKLDELQKPKHIIKYIGMGAPGPVDMAAG
VVYETVNLGWKNYALKNHLETETGIPAVIENDANIAALGEMWKGAGDGAKDVILVTLGTGVGGGIIANGEIVHGINGAGG
EIGHICSIPEGGAPCNCGKTGCIETIASATGIVRIAKEKIANAKKTTRLKATEQLSARDVFEAAGENDEIALEVVDYVAK
HLGLVLGNLASSLNPSKIVLGGGVSRAGELLRSKVEKTFRKCAFPRAAQAADISIAALGNDAGVIGGAWIAKNEWLKHQN
C

Specific function: May Be A Regulator In Specific Sugar Metabolism. May Influence The Rate Of Glucose Uptake Or Glycolysis In Cells In The Rich Medium. [C]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family

Homologues:

Organism=Homo sapiens, GI298566325, Length=262, Percent_Identity=29.0076335877863, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI298566315, Length=262, Percent_Identity=29.0076335877863, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI190014632, Length=262, Percent_Identity=29.0076335877863, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4885285, Length=262, Percent_Identity=29.0076335877863, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787878, Length=316, Percent_Identity=29.1139240506329, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786891, Length=274, Percent_Identity=30.2919708029197, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87081733, Length=317, Percent_Identity=30.9148264984227, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787363, Length=315, Percent_Identity=26.3492063492064, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082230, Length=261, Percent_Identity=29.5019157088123, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082127, Length=233, Percent_Identity=28.3261802575107, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790522, Length=326, Percent_Identity=25.7668711656442, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLK_BACSU (P54495)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B69632
- RefSeq:   NP_390365.2
- ProteinModelPortal:   P54495
- SMR:   P54495
- EnsemblBacteria:   EBBACT00000001122
- GeneID:   938206
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24850
- NMPDR:   fig|224308.1.peg.2489
- GenoList:   BSU24850
- GeneTree:   EBGT00050000001858
- HOGENOM:   HBG730260
- PhylomeDB:   P54495
- ProtClustDB:   CLSK2391277
- BioCyc:   BSUB:BSU24850-MONOMER
- BioCyc:   MetaCyc:MONOMER-5981
- BRENDA:   2.7.1.2
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR000600
- InterPro:   IPR004654
- TIGRFAMs:   TIGR00744

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.2

Molecular weight: Translated: 33604; Mature: 33604

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEIWFAGIDLGGTTIKLAFINQYGEIQHKWEVPTDKTGDTITVTIAKTIDSKLDELQKP
CCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCH
KHIIKYIGMGAPGPVDMAAGVVYETVNLGWKNYALKNHLETETGIPAVIENDANIAALGE
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCEEHHHH
MWKGAGDGAKDVILVTLGTGVGGGIIANGEIVHGINGAGGEIGHICSIPEGGAPCNCGKT
HHCCCCCCCCEEEEEEECCCCCCCEEECCEEEECCCCCCCCCCCEECCCCCCCCCCCCCH
GCIETIASATGIVRIAKEKIANAKKTTRLKATEQLSARDVFEAAGENDEIALEVVDYVAK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HLGLVLGNLASSLNPSKIVLGGGVSRAGELLRSKVEKTFRKCAFPRAAQAADISIAALGN
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
DAGVIGGAWIAKNEWLKHQNC
CCCEECCEEEECCCCCCCCCC
>Mature Secondary Structure
MDEIWFAGIDLGGTTIKLAFINQYGEIQHKWEVPTDKTGDTITVTIAKTIDSKLDELQKP
CCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCH
KHIIKYIGMGAPGPVDMAAGVVYETVNLGWKNYALKNHLETETGIPAVIENDANIAALGE
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCEEHHHH
MWKGAGDGAKDVILVTLGTGVGGGIIANGEIVHGINGAGGEIGHICSIPEGGAPCNCGKT
HHCCCCCCCCEEEEEEECCCCCCCEEECCEEEECCCCCCCCCCCEECCCCCCCCCCCCCH
GCIETIASATGIVRIAKEKIANAKKTTRLKATEQLSARDVFEAAGENDEIALEVVDYVAK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HLGLVLGNLASSLNPSKIVLGGGVSRAGELLRSKVEKTFRKCAFPRAAQAADISIAALGN
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
DAGVIGGAWIAKNEWLKHQNC
CCCEECCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 9620975