BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is mmgB

Identifier: 255767531

GI number: 255767531

Start: 2511973

End: 2512836

Strand: Reverse

Name: mmgB

Synonym: BSU24160

Alternate gene names: 255767531

Gene position: 2512836-2511973 (Counterclockwise)

Preceding gene: 16079473

Following gene: 255767530

Centisome position: 59.61

GC content: 48.26

Gene sequence:

>864_bases
ATGGAAATCAAACAAATCATGGTAGCTGGCGCAGGTCAGATGGGGAGCGGAATTGCTCAAACAGCCGCCGACGCGGGCTT
TTATGTGCGGATGTATGATGTGAATCCAGAGGCCGCGGAGGCAGGATTGAAACGGCTGAAGAAACAGCTGGCCCGTGATG
CTGAGAAAGGAAAAAGGACCGAGACGGAAGTGAAGAGCGTAATCAACCGCATTTCGATTTCTCAAACACTTGAGGAGGCA
GAGCATGCGGACATTGTGATTGAGGCTATCGCAGAAAACATGGCGGCAAAAACTGAGATGTTTAAAACACTTGATCGCAT
TTGCCCGCCTCATACGATTTTGGCCAGCAATACATCTTCCTTGCCTATTACAGAAATCGCTGCTGTAACAAACCGGCCTC
AACGGGTTATTGGCATGCATTTTATGAATCCCGTCCCTGTAATGAAGCTGGTAGAAGTGATTCGAGGCTTGGCTACATCA
GAAGAAACGGCCTTAGATGTTATGGCATTAGCGGAAAAGATGGGGAAAACAGCGGTAGAAGTCAATGATTTTCCTGGGTT
TGTTTCCAACCGTGTGCTTCTTCCAATGATTAATGAAGCCATCTATTGCGTGTATGAGGGAGTGGCGAAGCCGGAGGCAA
TAGATGAAGTGATGAAGCTGGGCATGAATCATCCGATGGGTCCGCTTGCATTAGCGGATTTTATCGGACTGGATACGTGT
TTATCAATTATGGAAGTCCTTCACTCAGGCCTTGGCGATTCCAAATACCGTCCTTGCCCGCTGCTCCGCAAGTATGTCAA
AGCAGGCTGGCTTGGCAAAAAGAGCGGACGCGGTTTTTATGACTATGAGGAGAAGACTTCCTGA

Upstream 100 bases:

>100_bases
GGGAGGGGGTCTGGGCGTTGCCGCTATTTGCAGCGGGGCAGCACAGGGAGACGCCGTCTTAGTGCAGGTTCATTAAAACA
TGATGAAAAAGGGGATTTGA

Downstream 100 bases:

>100_bases
AGTCTATTCCATCCGGGGGTGGCCGAGTTGCATGTAACCCAAGAGCAAGTCATGATGCGAAAAATGGTGCGTGATTTTGC
ACGAAAAGAAATTGCTCCCG

Product: 3-hydroxybutyryl-CoA dehydrogenase

Products: NA

Alternate protein names: Beta-hydroxybutyryl-CoA dehydrogenase; BHBD

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MEIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRTETEVKSVINRISISQTLEEA
EHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSSLPITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATS
EETALDVMALAEKMGKTAVEVNDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTC
LSIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEEKTS

Sequences:

>Translated_287_residues
MEIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRTETEVKSVINRISISQTLEEA
EHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSSLPITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATS
EETALDVMALAEKMGKTAVEVNDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTC
LSIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEEKTS
>Mature_287_residues
MEIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRTETEVKSVINRISISQTLEEA
EHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSSLPITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATS
EETALDVMALAEKMGKTAVEVNDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTC
LSIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEEKTS

Specific function: Butyrate/butanol-producing pathway. Phenylacetic acid aerobic catabolism. [C]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family

Homologues:

Organism=Homo sapiens, GI296179429, Length=288, Percent_Identity=41.6666666666667, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI296179427, Length=306, Percent_Identity=39.8692810457516, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI20127408, Length=284, Percent_Identity=37.6760563380282, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI261878539, Length=294, Percent_Identity=33.6734693877551, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI68989263, Length=294, Percent_Identity=33.6734693877551, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI115430219, Length=230, Percent_Identity=30, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1787661, Length=283, Percent_Identity=38.86925795053, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI1788682, Length=266, Percent_Identity=39.4736842105263, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1790281, Length=268, Percent_Identity=36.9402985074627, Blast_Score=161, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17553560, Length=288, Percent_Identity=39.9305555555556, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17549919, Length=289, Percent_Identity=39.7923875432526, Blast_Score=208, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17563036, Length=288, Percent_Identity=37.1527777777778, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI25144276, Length=283, Percent_Identity=36.7491166077738, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17508951, Length=283, Percent_Identity=36.7491166077738, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17508953, Length=283, Percent_Identity=36.7491166077738, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17558304, Length=284, Percent_Identity=35.2112676056338, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71985923, Length=285, Percent_Identity=32.6315789473684, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71985930, Length=285, Percent_Identity=28.0701754385965, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI193203623, Length=195, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533614, Length=195, Percent_Identity=26.1538461538462, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921000, Length=283, Percent_Identity=34.6289752650177, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24583077, Length=283, Percent_Identity=34.6289752650177, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24583079, Length=283, Percent_Identity=34.6289752650177, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI45549573, Length=239, Percent_Identity=28.0334728033473, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20129993, Length=225, Percent_Identity=25.3333333333333, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HBD_BACSU (P45856)

Other databases:

- EMBL:   U29084
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   C69658
- RefSeq:   NP_390296.2
- ProteinModelPortal:   P45856
- SMR:   P45856
- EnsemblBacteria:   EBBACT00000000904
- GeneID:   938660
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24160
- GenoList:   BSU24160
- GeneTree:   EBGT00050000000905
- HOGENOM:   HBG691737
- PhylomeDB:   P45856
- ProtClustDB:   CLSK2539629
- BioCyc:   BSUB:BSU24160-MONOMER
- BRENDA:   1.1.1.157
- InterPro:   IPR022694
- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PIRSF:   PIRSF000105

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.157

Molecular weight: Translated: 31394; Mature: 31394

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00067 3HCDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRT
CCHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCH
ETEVKSVINRISISQTLEEAEHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LPITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATSEETALDVMALAEKMGKTAVE
CCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEE
VNDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTC
ECCCCCHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LSIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEEKTS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRT
CCHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCH
ETEVKSVINRISISQTLEEAEHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LPITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATSEETALDVMALAEKMGKTAVE
CCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEE
VNDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTC
ECCCCCHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LSIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEEKTS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377