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The map label for this gene is yoaE

Identifier: 255767437

GI number: 255767437

Start: 2025400

End: 2027442

Strand: Direct

Name: yoaE

Synonym: BSU18570

Alternate gene names: 255767437

Gene position: 2025400-2027442 (Clockwise)

Preceding gene: 255767436

Following gene: 16078919

Centisome position: 48.05

GC content: 46.06

Gene sequence:

>2043_bases
ATGAGATCTTTTGCCACACAACAAAACGGCATTTTCAAATCCGTTTGCTCGCTGGACTGCCCGGATCAGTGCGGATTGTT
AATACATAAAAAAGACGGGAAAATCGTAAAAGTGCAAGGAGACCCTGATCATCCTGTCACAGCTGGAAACATATGCAACA
AAGTCCGGAATATGACGGAGCGCATCTATGATGAAAAGCGGCTGACTACACCGCTCAAACGAACAGGTGCGAAAGGCCAA
GCGATATTTGAACCGATTTCCTGGAAAGAGGCAATCGATACGATCACCTCCCGCTGGAAACAGCTGATTGATGAAGAGGG
AGCTGAAAGCATACTTCCTTACAGCTTTTACGGAAATATGGGGAAATTGACAGCTGAAGGAATGGACCGCCGCTTTTTTT
ACCGCATGGGGTCAAGCCAGCTTGAACGGACGATTTGCAGCAAAGCGGGATCTGAAGGCTATAAATATACAATGGGCATA
AGCGCGGGAATCGATCCTGAGGAAACCGTTCATACAAAACTATTTATTTTTTGGGGAATCAACGCGGTCAGCACAAATAT
GCACCAAATTACGATCGCCCAAAAAGCGCGAAAAAAAGGTGCCAAAATTGTCGTGATCGATGTGCATAAAAATCAAACAG
GACGTCTTGCAGATTGGTTTATCCCAATAAAACCGGGAACCGACAGTGCACTTGCTCTTGGCATCATGCACATTTTATTT
AAAGAAAACCTTCATGATGAAGCATTTCTGTCCGAGTACACGGTTGGCTATGAAGAGCTCCGTGAACACGTGAAACAATA
TGATCCTGAAAAGGTATCGACGATCACCGGTGTCAGTACCGAAGATATTTACCGACTGGCAAAAATGTACGGGGAAACCT
CCCCTTCTTTTATCAGAATCGGAAACGGACCCCAGCATCACGATAACGGGGGGATGATTGTAAGGACGATTGCCTGTCTT
CCGGCGATCACCGGCCAATGGCTGCACACAGGGGGCGGCGCAATTAAGCATAACAGCGGAATTTTAGAATATAACACAAA
TGCCTTGCAGCGGCCCGACTTATTGAAAGGGCGCACCCCGAGGTCGTTTAATATGAATCAGCTTGGCAGGGTGCTTTTGG
AAACAGACCCGCCGATTCGCTCTCTATTTATTTACGGAACGAACCCTGCCGTCGTCGCGCCCGAAGCAAACAAAGTCAGA
CAGGGCTTGCTGCGGGAAGACTTGTTTACTGTGGTTCATGATTTGTTTTTAACGGAAACGGCTGCATATGCGGACATTGT
ACTGCCTGCAACATCCGCTTTTGAAAATACTGATTTTTACACCTCCTACTGGCACCATTACATTCAGCTTCAGCAGCCGG
TGATTGAGAGATATGGGGAAAGCAAGTCTAACACTGAGGTTTTCCGATTACTGGCTGAAGCCATGGGATTCACAGACCAG
GAGCTTAAAGATTCAGATGAAGTATTAATCCGCCAGGCGCTTGACCATCCAGATAATCCGCATTTGGCAGAGATTGATTA
CGATTCACTTACTAAGCATTCATTTATGAAAGCGAAACGTGAAAAGCCGCTGTTTCCGGGCGAACTTCCAACGCCAAGCG
GAAAAATAGAATTATATTCAGAAAAAATGAAACAAGACGGCTTCCCTGCTTTACCGACATATACACCTCTTGTAACCGAT
AATGAACATCCGTTTATGTATGTTCCGGGGCCAAACCATAATTTCTTAAACTCAACATTTTCCAATAATGAAAAACACAT
CAAGCTTGAAAAGACGCCAAAGCTCTTTATCAACACAAAAGATGCTGAAAAACACGGAATCGTTGATGGGGCACCTGTAC
GAATTTGGAACAGCCGGGGAGAATGCGAGCTGACCGCTGCAGTTGGAGAACAAGTGCTGCCAGGTGTCGTTGTCAGCCAA
GGATTATGGGCGGATGAACAAGGAAAAAAACAGCTTGTAAACGCGCTAACCCCAGATCGCTTGTCCGATATGGGCGGCGG
CGCCACCTTTTTTTCAGGCCGTGTACAGATTGAAAAGGTTTGA

Upstream 100 bases:

>100_bases
AGACTCAGATTGATGTCAATCTCTTTTTTTCAGAATTGACGTTATCATCGGAAATGTTATAATTCTTACAAAATATCTAA
CTAGAGAGAGGAACTGGGCC

Downstream 100 bases:

>100_bases
AAAATAATGAAACCCGGAGTATGCCAAGCCCGTATAACATAACATCGACATAATGGAGGCATACACATGGACGTTTTTTT
AGGAATTGGTATTGCTTTGG

Product: molybdopterin cofactor oxido-reductase

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MRSFATQQNGIFKSVCSLDCPDQCGLLIHKKDGKIVKVQGDPDHPVTAGNICNKVRNMTERIYDEKRLTTPLKRTGAKGQ
AIFEPISWKEAIDTITSRWKQLIDEEGAESILPYSFYGNMGKLTAEGMDRRFFYRMGSSQLERTICSKAGSEGYKYTMGI
SAGIDPEETVHTKLFIFWGINAVSTNMHQITIAQKARKKGAKIVVIDVHKNQTGRLADWFIPIKPGTDSALALGIMHILF
KENLHDEAFLSEYTVGYEELREHVKQYDPEKVSTITGVSTEDIYRLAKMYGETSPSFIRIGNGPQHHDNGGMIVRTIACL
PAITGQWLHTGGGAIKHNSGILEYNTNALQRPDLLKGRTPRSFNMNQLGRVLLETDPPIRSLFIYGTNPAVVAPEANKVR
QGLLREDLFTVVHDLFLTETAAYADIVLPATSAFENTDFYTSYWHHYIQLQQPVIERYGESKSNTEVFRLLAEAMGFTDQ
ELKDSDEVLIRQALDHPDNPHLAEIDYDSLTKHSFMKAKREKPLFPGELPTPSGKIELYSEKMKQDGFPALPTYTPLVTD
NEHPFMYVPGPNHNFLNSTFSNNEKHIKLEKTPKLFINTKDAEKHGIVDGAPVRIWNSRGECELTAAVGEQVLPGVVVSQ
GLWADEQGKKQLVNALTPDRLSDMGGGATFFSGRVQIEKV

Sequences:

>Translated_680_residues
MRSFATQQNGIFKSVCSLDCPDQCGLLIHKKDGKIVKVQGDPDHPVTAGNICNKVRNMTERIYDEKRLTTPLKRTGAKGQ
AIFEPISWKEAIDTITSRWKQLIDEEGAESILPYSFYGNMGKLTAEGMDRRFFYRMGSSQLERTICSKAGSEGYKYTMGI
SAGIDPEETVHTKLFIFWGINAVSTNMHQITIAQKARKKGAKIVVIDVHKNQTGRLADWFIPIKPGTDSALALGIMHILF
KENLHDEAFLSEYTVGYEELREHVKQYDPEKVSTITGVSTEDIYRLAKMYGETSPSFIRIGNGPQHHDNGGMIVRTIACL
PAITGQWLHTGGGAIKHNSGILEYNTNALQRPDLLKGRTPRSFNMNQLGRVLLETDPPIRSLFIYGTNPAVVAPEANKVR
QGLLREDLFTVVHDLFLTETAAYADIVLPATSAFENTDFYTSYWHHYIQLQQPVIERYGESKSNTEVFRLLAEAMGFTDQ
ELKDSDEVLIRQALDHPDNPHLAEIDYDSLTKHSFMKAKREKPLFPGELPTPSGKIELYSEKMKQDGFPALPTYTPLVTD
NEHPFMYVPGPNHNFLNSTFSNNEKHIKLEKTPKLFINTKDAEKHGIVDGAPVRIWNSRGECELTAAVGEQVLPGVVVSQ
GLWADEQGKKQLVNALTPDRLSDMGGGATFFSGRVQIEKV
>Mature_680_residues
MRSFATQQNGIFKSVCSLDCPDQCGLLIHKKDGKIVKVQGDPDHPVTAGNICNKVRNMTERIYDEKRLTTPLKRTGAKGQ
AIFEPISWKEAIDTITSRWKQLIDEEGAESILPYSFYGNMGKLTAEGMDRRFFYRMGSSQLERTICSKAGSEGYKYTMGI
SAGIDPEETVHTKLFIFWGINAVSTNMHQITIAQKARKKGAKIVVIDVHKNQTGRLADWFIPIKPGTDSALALGIMHILF
KENLHDEAFLSEYTVGYEELREHVKQYDPEKVSTITGVSTEDIYRLAKMYGETSPSFIRIGNGPQHHDNGGMIVRTIACL
PAITGQWLHTGGGAIKHNSGILEYNTNALQRPDLLKGRTPRSFNMNQLGRVLLETDPPIRSLFIYGTNPAVVAPEANKVR
QGLLREDLFTVVHDLFLTETAAYADIVLPATSAFENTDFYTSYWHHYIQLQQPVIERYGESKSNTEVFRLLAEAMGFTDQ
ELKDSDEVLIRQALDHPDNPHLAEIDYDSLTKHSFMKAKREKPLFPGELPTPSGKIELYSEKMKQDGFPALPTYTPLVTD
NEHPFMYVPGPNHNFLNSTFSNNEKHIKLEKTPKLFINTKDAEKHGIVDGAPVRIWNSRGECELTAAVGEQVLPGVVVSQ
GLWADEQGKKQLVNALTPDRLSDMGGGATFFSGRVQIEKV

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family

Homologues:

Organism=Escherichia coli, GI87081797, Length=777, Percent_Identity=31.4028314028314, Blast_Score=264, Evalue=2e-71,
Organism=Escherichia coli, GI1787870, Length=761, Percent_Identity=30.4862023653088, Blast_Score=239, Evalue=6e-64,
Organism=Escherichia coli, GI171474008, Length=773, Percent_Identity=28.7192755498059, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI3868721, Length=681, Percent_Identity=24.5227606461087, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI145693196, Length=729, Percent_Identity=24.9657064471879, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI87081994, Length=765, Percent_Identity=23.1372549019608, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1787231, Length=712, Percent_Identity=22.752808988764, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788534, Length=564, Percent_Identity=22.5177304964539, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI3868719, Length=409, Percent_Identity=22.9828850855746, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787778, Length=303, Percent_Identity=25.0825082508251, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YOAE_BACSU (C0SP82)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_389739.2
- ProteinModelPortal:   C0SP82
- SMR:   C0SP82
- EnsemblBacteria:   EBBACT00000001875
- GeneID:   940113
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18570
- GenoList:   BSU18570
- GeneTree:   EBGT00070000031948
- ProtClustDB:   CLSK887382
- BioCyc:   BSUB:BSU18570-MONOMER
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: 1.2.1.2

Molecular weight: Translated: 76168; Mature: 76168

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFATQQNGIFKSVCSLDCPDQCGLLIHKKDGKIVKVQGDPDHPVTAGNICNKVRNMTE
CCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RIYDEKRLTTPLKRTGAKGQAIFEPISWKEAIDTITSRWKQLIDEEGAESILPYSFYGNM
HHHHHHHHCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
GKLTAEGMDRRFFYRMGSSQLERTICSKAGSEGYKYTMGISAGIDPEETVHTKLFIFWGI
CCEECCCCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHEEEEEEEECC
NAVSTNMHQITIAQKARKKGAKIVVIDVHKNQTGRLADWFIPIKPGTDSALALGIMHILF
HHHCCCCEEEEEHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
KENLHDEAFLSEYTVGYEELREHVKQYDPEKVSTITGVSTEDIYRLAKMYGETSPSFIRI
HCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHCCCCCCEEEE
GNGPQHHDNGGMIVRTIACLPAITGQWLHTGGGAIKHNSGILEYNTNALQRPDLLKGRTP
CCCCCCCCCCCEEEHHHHHHHHHCCCCEECCCCEEEECCCEEEECCCCCCCCHHHCCCCC
RSFNMNQLGRVLLETDPPIRSLFIYGTNPAVVAPEANKVRQGLLREDLFTVVHDLFLTET
CCCCHHHHHHEEECCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAYADIVLPATSAFENTDFYTSYWHHYIQLQQPVIERYGESKSNTEVFRLLAEAMGFTDQ
HHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCH
ELKDSDEVLIRQALDHPDNPHLAEIDYDSLTKHSFMKAKREKPLFPGELPTPSGKIELYS
HHCCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHH
EKMKQDGFPALPTYTPLVTDNEHPFMYVPGPNHNFLNSTFSNNEKHIKLEKTPKLFINTK
HHHHCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECC
DAEKHGIVDGAPVRIWNSRGECELTAAVGEQVLPGVVVSQGLWADEQGKKQLVNALTPDR
CCHHCCCCCCCCEEEECCCCCEEEEHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCCHH
LSDMGGGATFFSGRVQIEKV
HHHCCCCEEEEECEEEEEEC
>Mature Secondary Structure
MRSFATQQNGIFKSVCSLDCPDQCGLLIHKKDGKIVKVQGDPDHPVTAGNICNKVRNMTE
CCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RIYDEKRLTTPLKRTGAKGQAIFEPISWKEAIDTITSRWKQLIDEEGAESILPYSFYGNM
HHHHHHHHCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
GKLTAEGMDRRFFYRMGSSQLERTICSKAGSEGYKYTMGISAGIDPEETVHTKLFIFWGI
CCEECCCCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHEEEEEEEECC
NAVSTNMHQITIAQKARKKGAKIVVIDVHKNQTGRLADWFIPIKPGTDSALALGIMHILF
HHHCCCCEEEEEHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
KENLHDEAFLSEYTVGYEELREHVKQYDPEKVSTITGVSTEDIYRLAKMYGETSPSFIRI
HCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHCCCCCCEEEE
GNGPQHHDNGGMIVRTIACLPAITGQWLHTGGGAIKHNSGILEYNTNALQRPDLLKGRTP
CCCCCCCCCCCEEEHHHHHHHHHCCCCEECCCCEEEECCCEEEECCCCCCCCHHHCCCCC
RSFNMNQLGRVLLETDPPIRSLFIYGTNPAVVAPEANKVRQGLLREDLFTVVHDLFLTET
CCCCHHHHHHEEECCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAYADIVLPATSAFENTDFYTSYWHHYIQLQQPVIERYGESKSNTEVFRLLAEAMGFTDQ
HHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCH
ELKDSDEVLIRQALDHPDNPHLAEIDYDSLTKHSFMKAKREKPLFPGELPTPSGKIELYS
HHCCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHH
EKMKQDGFPALPTYTPLVTDNEHPFMYVPGPNHNFLNSTFSNNEKHIKLEKTPKLFINTK
HHHHCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECC
DAEKHGIVDGAPVRIWNSRGECELTAAVGEQVLPGVVVSQGLWADEQGKKQLVNALTPDR
CCHHCCCCCCCCEEEECCCCCEEEEHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCCHH
LSDMGGGATFFSGRVQIEKV
HHHCCCCEEEEECEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377